Capillary electrophoresis for the determination of albumin binding capacity and free bilirubin in jaundiced neonates

2001 ◽  
Vol 25 (2) ◽  
pp. 50-54 ◽  
Author(s):  
Chap Y. Yeung ◽  
Ying S. Fung ◽  
Dong X. Sun
Keyword(s):  
Capillary Electrophoresis ◽  
Binding Capacity ◽  
Albumin Binding

Determination of Unbound Bilirubin in the Serum of Newborns

1974 ◽  
Vol 20 (7) ◽  
pp. 783-789 ◽  
Author(s):  
Jorgen Jacobsen ◽  
Richard P Wennberg
Keyword(s):  
Binding Capacity ◽  
Newborn Infants ◽  
Albumin Binding ◽  
Initial Oxidation ◽  
High Affinity ◽  
Normal Human ◽  
Unbound Bilirubin ◽  
Rate Limiting

Abstract An enzymatic assay is described for non-albuminbound bilirubin in the serum of newborn infants. Unbound bilirubin is oxidized to colorless compounds by ethyl hydroperoxide in the presence of horseradish peroxidase (EC 1.11.1.7), while albumin-bound bilirubin is protected from oxidation. Because the equilibrium between albumin and bilirubin occurs rapidly, the oxidation step is rate limiting, and the initial oxidation velocity of total bilirubin is proportional to the unbound bilirubin concentration. By titrating serum with bilirubin in vitro, the association constant and binding capacity of high-affinity sites for albumin binding can be determined. Normal human serum albumin tightly binds 1 mole of bilirubin per mole of albumin (binding constant, 2-4 x 108 liter/mol). Although weaker secondary binding occurs, the unbound bilirubin fraction increases rapidly after the high-affinity binding sites are saturated. Compromised newborns may have a decreased apparent binding capacity and (or) binding affinity. The method can be used to assess the risk of a jaundiced infant for bilirubin encephalopathy.

Determination of Mutation of C-myc Oncogene in Gastric Cancer by Capillary Electrophoresis

2012 ◽  
Vol 39 (11) ◽  
pp. 1695-1700
Author(s):  
Xi-Hui XIE ◽  
Rong WANG ◽  
Zheng-Ping JIA ◽  
Hua XIE ◽  
Ai-Mei ZHAMG ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Capillary Electrophoresis ◽  
Myc Oncogene

Determination of gambogic acid inGambogeby non-aqueous capillary electrophoresis

2015 ◽  
Vol 33 (2) ◽  
pp. 152
Author(s):  
Wanlu OU ◽  
Yujuan LI ◽  
Dongdong SHI ◽  
Feng QU
Keyword(s):  
Capillary Electrophoresis ◽  
Gambogic Acid
Sign in / Sign up

Export Citation Format

Share Document