Quantitative real-time polymerase chain reaction for monitoring minimal residual disease in patients with advanced indolent lymphomas treated with rituximab, fludarabine, mitoxantrone, and dexamethasone

2002 ◽  
Vol 29 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Andreas H. Sarris ◽  
Yunfang Jiang ◽  
Apostolia M. Tsimberidou ◽  
Athanasios Thomaides ◽  
George Z. Rassidakis ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Indolent Lymphomas ◽  
Minimal Residual

scholarly journals Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia

2015 ◽  
Vol 37 (6) ◽  
pp. 373-380 ◽  
Author(s):  
Francisco Danilo Ferreira Paula ◽  
Silvana Maria Elói-Santos ◽  
Sandra Guerra Xavier ◽  
Mônica Aparecida Ganazza ◽  
Patricia Yoshioka Jotta ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Acute Lymphoblastic Leukemia ◽  
Real Time ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Minimal Residual

Detection of Nucleophosmin 1 Mutations by Quantitative Real-Time Polymerase Chain Reaction Versus Capillary Electrophoresis: A Comparative Study

2011 ◽  
Vol 135 (8) ◽  
pp. 994-1000 ◽  
Author(s):  
Fareed H Barakat ◽  
Rajyalakshmi Luthra ◽  
C. Cameron Yin ◽  
Bedia A Barkoh ◽  
Seema Hai ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Capillary Electrophoresis ◽  
Real Time ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Qpcr Assay ◽  
Analytical Sensitivity ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Minimal Residual

Context.—Nucleophosmin 1 (NPM1) is the most commonly mutated gene in acute myeloid leukemia. Detection of NPM1 mutations is useful for stratifying patients for therapy, predicting prognosis, and assessing for minimal residual disease. Several methods have been developed to rapidly detect NPM1 mutations in genomic DNA and/or messenger RNA specimens. Objective.—To directly compare a quantitative real-time polymerase chain reaction (qPCR) assay with a widely used capillary electrophoresis assay for detecting NPM1 mutations. Design.—We adopted and modified a qPCR assay designed to detect the 6 most common NPM1 mutations and performed the assay in parallel with capillary electrophoresis assay in 207 bone marrow aspirate or peripheral blood samples from patients with a range of hematolymphoid neoplasms. Results.—The qPCR assay demonstrated a higher analytical sensitivity than the capillary electrophoresis 1/1000 versus 1/40, respectively. The capillary electrophoresis assay generated 10 equivocal results that needed to be repeated, whereas the qPCR assay generated only 1 equivocal result. After test conditions were optimized, the qPCR and capillary electrophoresis methods produced 100% concordant results, 85 positive and 122 negative. Conclusions.—Given the higher analytical sensitivity and specificity of the qPCR assay, that assay is less likely to generate equivocal results than the capillary electrophoresis assay. Moreover, the qPCR assay is quantitative, faster, cheaper, less prone to contamination, and well suited for monitoring minimal residual disease.

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