In vitro Migration of Cytotrophoblasts Through a Decidual Endothelial Cell Monolayer: The Role of Matrix Metalloproteinases

Placenta ◽  
2003 ◽  
Vol 24 (4) ◽  
pp. 306-315 ◽  
Author(s):  
S Campbell ◽  
J Rowe ◽  
C.J Jackson ◽  
E.D.M Gallery
Keyword(s):  
Endothelial Cell ◽  
Matrix Metalloproteinases ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer

Role of thrombin in endothelial cell monolayer repair in vitro

1994 ◽  
Vol 20 (4) ◽  
pp. 621-628 ◽  
Author(s):  
Paul J. DiMuzio ◽  
Kerri J. Pratt ◽  
Pauline K. Park ◽  
R.Anthony Carabasi
Keyword(s):  
Endothelial Cell ◽  
Cell Monolayer ◽  
Endothelial Cell Monolayer

scholarly journals ATP increases the migration of microglia across the brain endothelial cell monolayer

2016 ◽  
Vol 36 (2) ◽  
Author(s):  
Tomoji Maeda ◽  
Manato Inagaki ◽  
Yu Fujita ◽  
Takehiro Kimoto ◽  
Chiaki Tanabe-Fujimura ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Matrix Metalloproteinases ◽  
Real Time ◽  
Culture System ◽  
Cell Monolayer ◽  
Brain Endothelial Cell ◽  
Endothelial Cell Monolayer ◽  
Microglial Migration ◽  
The Brain

To elucidate the mechanism of microglial migration across the blood–brain barrier (BBB), we developed an in vitro co-culture system and analysed real-time BBB integrity during transmigration. We show that ATP promotes microglia transmigration via a mechanism involving microglial matrix metalloproteinases (MMPs).

Characterization of an In Vitro Model to Study the Permeability of Human Arterial Endothelial Cell Monolayers

1988 ◽  
Vol 60 (02) ◽  
pp. 240-246 ◽  
Author(s):  
Erna G Langeler ◽  
Victor W M van Hinsbergh
Keyword(s):  
Endothelial Cell ◽  
Calcium Ions ◽  
Electrical Resistance ◽  
Serum Proteins ◽  
Cell Monolayer ◽  
Extracellular Calcium ◽  
Endothelial Cell Monolayer ◽  
Passage Rate ◽  
Cell Monolayers

SummaryA model has been developed to study the transport of fluid and macromolecules through human arterial umbilical cord endothelial cell monolayers in vitro. Cells were cultured on fibronectin- coated polycarbonate filters and formed within a few days a tight monolayer, with an electrical resistance of 17 ± 4 Ohm · cm2. The cells were connected by close cell contacts with tight junctions. The passáge-rate of horse radish peroxidase (HRP) through these filters was 20-40 fold lower than through filters without an endothelial monolayer. The continuous presence of 10% human serum was needed to maintain the electrical resistance of the monolayer and its barrier function towards macromolecules. Chelation of extracellular calcium resulted in an increased permeability and a decreased electrical resistance of the monolayer. This process was reversible by re-addition of calcium ions to the cells. The permeation rate of dextrans of various molecular weights (9-480 kD) was inversely related to the molecular mass of the molecule. No difference was measured between the passage rate of dextran of 480 kD and dextran of 2,000 kD. Incubation of the endothelial cell monolayer with 2-deoxy-D-glucose resulted in a decreased permeability but it had no effect on electrical resistance. This suggests that the passage-process is energy- dependent.Fluid permeation through the endothelial cell monolayer on filters was measured in a perfusion chamber under 20 mmHg hydrostatic pressure. It was decreased by the presence of serum proteins and responded reversibly on the chelation and readdition of extracellular calcium ions.

scholarly journals Viable “Haemophilus somnus” Induces Myosin Light-Chain Kinase-Dependent Decrease in Brain Endothelial Cell Monolayer Resistance

2007 ◽  
Vol 75 (9) ◽  
pp. 4572-4581 ◽  
Author(s):  
E. Behling-Kelly ◽  
David McClenahan ◽  
K. S. Kim ◽  
C. J. Czuprynski
Keyword(s):  
Endothelial Cell ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Cell Monolayer ◽  
Brain Endothelial Cell ◽  
Endothelial Cell Monolayer ◽  
Necrosis Factor Alpha ◽  
Haemophilus Somnus

ABSTRACT “Haemophilus somnus” causes thrombotic meningoencephalitis in cattle. Our laboratory has previously reported that H. somnus has the ability to adhere to, but not invade, bovine brain endothelial cells (BBEC) in vitro. The goal of this study was to determine if H. somnus alters brain endothelial cell monolayer integrity in vitro, in a manner that would be expected to contribute to inflammation of the central nervous system (CNS). Monolayer integrity was monitored by measuring transendothelial electrical resistance (TEER) and albumin flux. BBEC incubated with H. somnus underwent rapid cytoskeletal rearrangement, significant increases in albumin flux, and reductions in TEER. Decreased monolayer TEER was preceded by phosphorylation of the myosin regulatory light chain and was partially dependent on tumor necrosis factor alpha and myosin light-chain kinase but not interleukin-1β. Neither heat-killed H. somnus, formalin-fixed H. somnus, nor purified lipooligosaccharide altered monolayer integrity within a 2-h incubation period, whereas conditioned medium from H. somnus-treated BBEC caused a modest reduction in TEER. The data from this study support the hypothesis that viable H. somnus alters integrity of the blood-brain barrier by promoting contraction of BBEC and increasing paracellular permeability of the CNS vasculature.

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