Identity of Human Trophoblast Cell Lines: Lessons From ED27

Placenta ◽  
2002 ◽  
Vol 23 (1) ◽  
pp. 2
Keyword(s):  
Cell Lines ◽  
Trophoblast Cell ◽  
Human Trophoblast

scholarly journals cAMP efflux from human trophoblast cell lines: a role for multidrug resistance protein (MRP)1 transporter

2010 ◽  
Vol 16 (7) ◽  
pp. 481-491 ◽  
Author(s):  
C. Biondi ◽  
M. E. Ferretti ◽  
L. Lunghi ◽  
S. Medici ◽  
F. Cervellati ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cell Lines ◽  
Trophoblast Cell ◽  
Multidrug Resistance Protein ◽  
Human Trophoblast ◽  
Resistance Protein

scholarly journals Modelling preeclampsia: a comparative analysis of the common human trophoblast cell lines

2020 ◽  
Author(s):  
Jiawu Zhao ◽  
Rebecca P. Chow ◽  
Rebecca H. McLeese ◽  
Michelle B. Hookham ◽  
Timothy J. Lyons ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Cell Lines ◽  
Trophoblast Cell ◽  
Human Trophoblast ◽  
The Common

Characteristics of Transcription-regulatory Elements for Gene Expression from Plasmid Vectors in Human Trophoblast Cell Lines

Placenta ◽  
2006 ◽  
Vol 27 (9-10) ◽  
pp. 934-938 ◽  
Author(s):  
E. Komiya ◽  
M. Kondoh ◽  
H. Mizuguchi ◽  
M. Fujii ◽  
N. Utoguchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Regulatory Elements ◽  
Trophoblast Cell ◽  
Plasmid Vectors ◽  
Human Trophoblast

scholarly journals Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE

Placenta ◽  
2017 ◽  
Vol 52 ◽  
pp. 17-20 ◽  
Author(s):  
Jill L. Reiter ◽  
Holli M. Drendel ◽  
Sujata Chakraborty ◽  
Megan M. Schellinger ◽  
Men-Jean Lee ◽  
...  
Keyword(s):  
Cell Lines ◽  
Trophoblast Cell ◽  
Human Trophoblast

scholarly journals New era of trophoblast research: integrating morphological and molecular approaches

2020 ◽  
Vol 26 (5) ◽  
pp. 611-633
Author(s):  
Shingo Io ◽  
Eiji Kondoh ◽  
Yoshitsugu Chigusa ◽  
Kaoru Kawasaki ◽  
Masaki Mandai ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Cell Lines ◽  
Human Placenta ◽  
Pregnancy Complications ◽  
Structural Characteristics ◽  
Trophoblast Cell ◽  
Placental Development ◽  
Human Trophoblast ◽  
Pubmed Database ◽  
Developmental Features

Abstract Many pregnancy complications are the result of dysfunction in the placenta. The pathogenic mechanisms of placenta-mediated pregnancy complications, however, are unclear. Abnormal placental development in these conditions begins in the first trimester, but no symptoms are observed during this period. To elucidate effective preventative treatments, understanding the differentiation and development of human placenta is crucial. This review elucidates the uniqueness of the human placenta in early development from the aspect of structural characteristics and molecular markers. We summarise the morphogenesis of human placenta based on human specimens and then compile molecular markers that have been clarified by immunostaining and RNA-sequencing data across species. Relevant studies were identified using the PubMed database and Google Scholar search engines up to March 2020. All articles were independently screened for eligibility by the authors based on titles and abstracts. In particular, the authors carefully examined literature on human placentation. This review integrates the development of human placentation from morphological approaches in comparison with other species and provides new insights into trophoblast molecular markers. The morphological features of human early placentation are described in Carnegie stages (CS), from CS3 (floating blastocyst) to CS9 (emerging point of tertiary villi). Molecular markers are described for each type of trophoblast involved in human placental development. We summarise the character of human trophoblast cell lines and explain how long-term culture system of human cytotrophoblast, both monolayer and spheroid, established in recent studies allows for the generation of human trophoblast cell lines. Due to differences in developmental features among species, it is desirable to understand early placentation in humans. In addition, reliable molecular markers that reflect normal human trophoblast are needed to advance trophoblast research. In the clinical setting, these markers can be valuable means for morphologically and functionally assessing placenta-mediated pregnancy complications and provide early prediction and management of these diseases.

Cell Culture Models of Human Trophoblast II: Trophoblast Cell Lines—A Workshop Report

Placenta ◽  
2001 ◽  
Vol 22 ◽  
pp. S104-S106 ◽  
Author(s):  
K.T. Shiverick ◽  
A. King ◽  
H.-G. Frank ◽  
G.St.J. Whitley ◽  
J.E. Cartwright ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Lines ◽  
Trophoblast Cell ◽  
Human Trophoblast ◽  
Workshop Report ◽  
Culture Models ◽  
Cell Culture Models

scholarly journals Investigation of human trophoblast invasion in vitro

2020 ◽  
Vol 26 (4) ◽  
pp. 501-513 ◽  
Author(s):  
Yassen Abbas ◽  
Margherita Y Turco ◽  
Graham J Burton ◽  
Ashley Moffett
Keyword(s):  
Cell Lines ◽  
First Trimester ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Trophoblast Invasion ◽  
Human Trophoblast ◽  
Uterine Environment ◽  
Microfluidic Assays ◽  
Invasion Assays

Abstract BACKGROUND In humans, inadequate trophoblast invasion into the decidua is associated with the ‘great obstetrical syndromes’ which include pre-eclampsia, foetal growth restriction (FGR) and stillbirth. The mechanisms regulating invasion remain poorly understood, although interactions with the uterine environment are clearly of central importance. Extravillous trophoblast (EVT) cells invade the uterus and transform the spiral arteries. Progress in understanding how they invade has been limited due to the lack of good in vitro models. Firstly, there are no non-malignant cell lines that have an EVT phenotype. Secondly, the invasion assays used are of limited use for the small numbers of primary EVT available from first-trimester placentas. We discuss recent progress in this field with the generation of new EVT lines and invasion assays using microfluidic technology. OBJECTIVE AND RATIONALE Our aim is to describe the established models used to study human trophoblast invasion in vivo and in vitro. The difficulties of obtaining primary cells and cell lines that recapitulate the phenotype of EVT are discussed together with the advantages and pitfalls of the different invasion assays. We compare these traditional end point assays to microfluidic assays where the dynamics of migration can be measured. SEARCH METHODS Relevant studies were identified by PubMed search, last updated on February 2020. A search was conducted to determine the number of journal articles published using the cell lines JEG-3, BeWo, JAR, HTR-8/Svneo, Swan-71 and primary human extravillous trophoblast in the last 5 years. OUTCOMES Deep trophoblast invasion into the maternal decidua is a particular feature of human pregnancy. This invasion needs to be finely regulated to allocate resources between mother and baby. A reliable source of EVT is needed to study in vitro how the uterine environment regulates this process. First, we critically discuss the issues with the trophoblast cell lines currently used; for example, most of them lack expression of the defining marker of EVT, HLA-G. Recently, advances in human stem cell and organoid technology have been applied to extraembryonic tissues to develop trophoblast cell lines that can grow in two (2D) and three dimensions (3D) and differentiate to EVT. This means that the ‘trophoblast’ cell lines currently in use should rapidly become obsolete. Second, we critically discuss the problems with assays to study trophoblast invasion. These lack physiological relevance and have simplified migration dynamics. Microfluidic assays are a powerful tool to study cell invasion because they require only a few cells, which are embedded in 3D in an extracellular matrix. Their major advantage is real-time monitoring of cell movement, enabling detailed analysis of the dynamics of trophoblast migration. WIDER IMPLICATIONS Trophoblast invasion in the first trimester of pregnancy remains poorly understood despite the importance of this process in the pathogenesis of pre-eclampsia, FGR, stillbirth and recurrent miscarriage. The new technologies described here will allow investigation into this critical process.

EG-VEGF enhances the EVT invasion via PROKR2 in the human trophoblast cell lines

Placenta ◽  
2021 ◽  
Vol 103 ◽  
pp. 255
Author(s):  
Kazumasa Tani ◽  
Sakurako Mishima ◽  
Akiko Ohira ◽  
Jota Maki ◽  
Takashi Mitsui ◽  
...  
Keyword(s):  
Cell Lines ◽  
Trophoblast Cell ◽  
Human Trophoblast
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