Effect of inflammatory cytokines on the metabolism of low-density lipoproteins by human vascular endothelial cells

Metabolism ◽  
2001 ◽  
Vol 50 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Richard L. Klein ◽  
João L. Ascenção ◽  
Marina Mironova ◽  
Yan Huang ◽  
Maria F. Lopes-Virella
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Cytokines ◽  
Vascular Endothelial Cells ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Vascular Endothelial

scholarly journals Low Density Lipoproteins Downregulate Lysyl Oxidase in Vascular Endothelial Cells and the Arterial Wall

2002 ◽  
Vol 22 (9) ◽  
pp. 1409-1414 ◽  
Author(s):  
Cristina Rodríguez ◽  
Berta Raposo ◽  
José Martínez-González ◽  
Laura Casaní ◽  
Lina Badimon
Keyword(s):  
Endothelial Cells ◽  
Arterial Wall ◽  
Vascular Endothelial Cells ◽  
Lysyl Oxidase ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Vascular Endothelial

Abstract 476: Attenuated Activities of Mitochondria in Vascular Endothelial Cells Exposed to Oxidized or Glycated Low Density Lipoprotein

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Garry X Shen ◽  
Subir K Roy Chowdhury
Keyword(s):  
Endothelial Cells ◽  
Respiratory Chain ◽  
Complex I ◽  
Vascular Endothelial Cells ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Mitochondrial Respiratory Chain ◽  
Low Density ◽  
Vascular Endothelial ◽  
Glycated Ldl

Hyperglycemia and dyslipoproteinemia are two major biochemical markers of diabetes. Elevated low density lipoprotein (LDL) is a classical risk factor for atherosclerotic cardiovascular disease. Our previous studies demonstrated that oxidized LDL (ox-LDL) and glycated LDL (gly-LDL) increased the generation of reactive oxygen species (ROS) in vascular endothelial cells. ROS is implicated in endothelial dysfunction and diabetic vascular complications. Mitochondria are an important source of ROS in the body. We hypothesize that ox-LDL or gly-LDL might affect the activity of mitochondrial respiratory chain. We evaluated the activities of mitochondrial respiratory chain complexes in porcine aortic endothelial cells (PAEC) using OROBORS oxygraph. The oxygraph was used as a highly sensitive tool to evaluate mitochondrial complex activity in freshly harvested and digitonin-permeabilized PAEC (for Complex I, the rotenone-sensitive oxidation of glutamate + malate in the presence of ADP; Complex II, antimycin A-sensitive oxidation of succinate; Complex IV, potassium cyanide-sensitive oxidation of ascorbate + TMPD). The oxygen consumption in Complex I, II and IV of PAEC was significantly decreased by >12 h of incubation with LDL, ox-LDL or gly-LDL compared to control cultures. Attenuated activity of succinate cytochrome C reductase was detected in EC treated with LDL, ox-LDL or gly-LDL for 24 h. Decreased levels of respiratory control ratio were detected in EC treated with LDL or ox-LDL for 6 h, but not for 2 h, compared to control. Impaired activity of mitochondrial complexes can cause electron leakage in the respiratory chain and substantially increase ROS formation. The findings suggest that oxidized or glycated LDL attenuates mitochondrial activities in vascular EC, which may contribute to increased ROS generation and endothelial dysfunction induced by the atherogenic lipoproteins (supported by operating grants from Canadian Institute of Health Research and Canadian Diabetes Association).

Sign in / Sign up

Export Citation Format

Share Document