Regulation of lipoprotein lipase and hormone-sensitive lipase activity and gene expression in adipose and muscle tissue by growth hormone treatment during weight loss in obese patients

Metabolism ◽  
2000 ◽  
Vol 49 (7) ◽  
pp. 906-911 ◽  
Author(s):  
Bjørn Richelsen ◽  
Steen B. Pedersen ◽  
Kurt Kristensen ◽  
Jens D. Børglum ◽  
Helene Nørrelund ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Weight Loss ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Growth Hormone Treatment ◽  
Hormone Treatment ◽  
Hormone Sensitive Lipase ◽  
Obese Patients ◽  
Hormone Sensitive

scholarly journals Mechanisms involved in the adaptations of the adipocyte adrenergic signal-transduction system and their modulation by growth hormone during the lactation cycle in the rat

1993 ◽  
Vol 289 (3) ◽  
pp. 845-851 ◽  
Author(s):  
R G Vernon ◽  
L Piperova ◽  
P W Watt ◽  
E Finley ◽  
S Lindsay-Watt
Keyword(s):  
Signal Transduction ◽  
Growth Hormone ◽  
Lipase Activity ◽  
Growth Hormone Treatment ◽  
Hormone Treatment ◽  
Hormone Sensitive Lipase ◽  
Signal Transduction System ◽  
Litter Removal ◽  
Hormone Sensitive ◽  
Lactation Cycle

The mechanisms responsible for the diminished lipolytic response of adipocytes to catecholamines after litter removal from lactating rats and their modulation by growth hormone have been investigated. Lactation, litter removal and growth-hormone treatment did not alter the ability of noradrenaline to activate protein kinase A (A-kinase), showing that the defect in signal transduction in rats after litter removal is after A-kinase. Litter removal had no effect on hormone-sensitive lipase activity itself, but the proportion of the lipase associated with the fat droplet was decreased; growth-hormone treatment increased hormone-sensitive lipase activity and the proportion associated with the fat droplet. In addition, a number of other adaptations in the beta-adrenergic signal-transduction system occur during the lactation cycle and in response to growth hormone treatment, including changes in receptor number, adenylate cyclase activity and cyclic AMP phosphodiesterase activity, but a defect in the ability of hormone-sensitive lipase to associate with the lipid droplet appears to be the major reason for the diminished response to catecholamines on litter removal.

Stimulation of human albumin synthesis and gene expression by growth hormone treatment

2001 ◽  
Vol 20 (1) ◽  
pp. 59-67 ◽  
Author(s):  
H. BARLE ◽  
L. RÅHLÉN ◽  
P. ESSÉN ◽  
M.A. McNURLAN ◽  
P.J. GARLICK ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Growth Hormone Treatment ◽  
Hormone Treatment ◽  
Human Albumin ◽  
Albumin Synthesis ◽  
Stimulation Of

Overexpression of Human Lipoprotein Lipase Increases Hormone-Sensitive Lipase Activity in Adipose Tissue of Mice

1995 ◽  
Vol 211 (3) ◽  
pp. 761-766 ◽  
Author(s):  
M. Shimada ◽  
S. Ishibashi ◽  
K. Yamamoto ◽  
M. Kawamura ◽  
Y. Watanabe ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive

Lipoprotein lipase in guinea-pig adrenals: activity, mRNA, immunolocalization and regulation by ACTH

1991 ◽  
Vol 129 (2) ◽  
pp. 213-NP ◽  
Author(s):  
M. Gåfvels ◽  
S. Vilaró ◽  
T. Olivecrona
Keyword(s):  
Guinea Pig ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Hepatic Lipase ◽  
Fold Increase ◽  
Serum Cortisol ◽  
Transitional Zone ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive ◽  
Cytoplasmic Immunoreaction

ABSTRACT Lipase activity in homogenates of guinea-pig adrenals was studied under conditions which exclude the hormone-sensitive lipase/cholesterol ester hydrolase. Antibody inhibition and chromatography on heparin–Sepharose showed that most of the activity was due to lipoprotein lipase (LPL), and that there was only a small amount of hepatic lipase activity. Northern blot analysis of total RNA demonstrated the same three adrenal LPL mRNA species (1·8, 3·1 and 3·5 kb) as were found in adipose tissue and heart. Hence, at least part of the LPL activity in adrenals is due to enzyme synthesized within the tissue. Immunolocalization showed that LPL was associated with the endothelium of blood vessels throughout the gland. In addition, there was cytoplasmic immunoreaction, suggesting that lipase was synthesized in a subpopulation of cells in the transitional zone between the fasciculata and reticularis layer of the cortex, particularly over lipid-filled cells. There was also intense immunofluorescence over scattered cells in the adrenal medulla. Treatment with an ACTH analogue depot (20 IU, i.m.) for 11 days induced a 12-fold increase in serum cortisol and increased adrenal weight 2·2-fold. The treatment induced increases in LPL mRNA (about twofold), LPL activity and in the number of cells in the adrenal cortex which gave an immunoreaction for LPL. Journal of Endocrinology (1991) 129, 213–220

Sign in / Sign up

Export Citation Format

Share Document