Rapid characterization of the genomovars of the Burkholderia cepacia complex by PCR-single-stranded conformational polymorphism (PCR-SSCP) analysis

2001 ◽  
Vol 48 (2) ◽  
pp. 129-134 ◽  
Author(s):  
J.E. Moore ◽  
B.C. Millar ◽  
X. Jiru ◽  
J. McCappin ◽  
M. Crowe ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Sscp Analysis ◽  
Burkholderia Cepacia Complex ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Rapid Characterization

scholarly journals Distinguishing Species of the Burkholderia cepacia Complex and Burkholderia gladioli by Automated Ribotyping

2000 ◽  
Vol 38 (5) ◽  
pp. 1876-1884 ◽  
Author(s):  
Sylvain Brisse ◽  
Cees M. Verduin ◽  
Dana Milatovic ◽  
Ad Fluit ◽  
Jan Verhoef ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex ◽  
Opportunistic Pathogens ◽  
Burkholderia Gladioli ◽  
Microbial Characterization ◽  
Automated Ribotyping ◽  
Level Cluster ◽  
Identification Analysis ◽  
Epidemiological Characterization

Several species belonging to the genus Burkholderia are clinically relevant, opportunistic pathogens that inhabit major environmental reservoirs. Consequently, the availability of means for adequate identification and epidemiological characterization of individual environmental or clinical isolates is mandatory. In the present communication we describe the use of the Riboprinter microbial characterization system (Qualicon, Warwick, United Kingdom) for automated ribotyping of 104 strains of Burkholderia species from diverse sources, including several publicly accessible collections. The main outcome of this analysis was that all strains were typeable and that strains of Burkholderia gladioli and of each species of the B. cepacia complex, includingB. multivorans, B. stabilis, and B. vietnamiensis, were effectively discriminated. Furthermore, different ribotypes were discerned within each species. Ribotyping results were in general agreement with strain classification based on restriction fragment analysis of 16S ribosomal amplicons, but the resolution of ribotyping was much higher. This enabled automated molecular typing below the species level. Cluster analysis of the patterns obtained by ribotyping (riboprints) showed that withinB. gladioli, B. multivorans, and B. cepacia genomovar VI, the different riboprints identified always clustered together. Riboprints of B. cepacia genomovars I and III, B. stabilis, and B. vietnamiensis did not show distinct clustering but rather exhibited the formation of loose assemblages within which several smaller, genomovar-specific clusters were delineated. Therefore, ribotyping proved useful for genomovar identification. Analysis of serial isolates from individual patients demonstrated that infection with a single ribotype had occurred, despite minor genetic differences that were detected by pulsed-field gel electrophoresis of DNA macrorestriction fragments. The automated approach allows very rapid and reliable identification and epidemiological characterization of strains and generates an easily manageable database suited for expansion with information on additional bacterial isolates.

Morphometric and molecular characterizations of schistosome populations in Estuaire province Gabon

2009 ◽  
Vol 84 (1) ◽  
pp. 81-85 ◽  
Author(s):  
R. Mintsa Nguema ◽  
K. Mengue Ngou Milama ◽  
M. Kombila ◽  
D. Richard-Lenoble ◽  
P. Tisseyre ◽  
...  
Keyword(s):  
Sscp Analysis ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism

AbstractThe aim of this study was to test the hypothesis of the presence of hybrids between Schistosoma guineensis and S. haematobium in the Estuaire province (Western Gabon). Egg morphometry and single-stranded conformational polymorphism (SSCP) analysis on adult worms were used in order to characterize the schistosome populations of two sites. The morphology of the eggs showed three morphotypes: S. haematobium, S. guineensis and intermediate morphotypes, but the eggs of the morphotype S. guineensis were smaller compared to the values found in the literature. Furthermore, the SSCP analysis of the adult schistosomes showed that all the patterns corresponded to that of S. haematobium and gave evidence that hybrids were absent from our samples.

scholarly journals Molecular Characterization of Burkholderia cepacia Complex Isolates Causing Bacterial Fruit Rot of Apricot

2010 ◽  
Vol 26 (3) ◽  
pp. 223-230 ◽  
Author(s):  
Bin Li ◽  
Yuan Fang ◽  
Guoqing Zhang ◽  
Rongrong Yu ◽  
Miaomiao Lou ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Burkholderia Cepacia ◽  
Fruit Rot ◽  
Burkholderia Cepacia Complex

Genome characterization of a novel Burkholderia cepacia complex genomovar isolated from dieback affected mango orchards

2013 ◽  
Vol 29 (11) ◽  
pp. 2033-2044 ◽  
Author(s):  
Asifullah Khan ◽  
Huma Asif ◽  
David J. Studholme ◽  
Ishtiaq A. Khan ◽  
M. Kamran Azim
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex ◽  
Genome Characterization

scholarly journals Characterization of the Burkholderia cenocepacia J2315 Surface-Exposed Immunoproteome

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 509 ◽  
Author(s):  
Sílvia A. Sousa ◽  
António M.M. Seixas ◽  
Manoj Mandal ◽  
Manuel J. Rodríguez-Ortega ◽  
Jorge H. Leitão
Keyword(s):  
Burkholderia Cepacia ◽  
Experimental Approach ◽  
Therapeutic Option ◽  
Peptide Identification ◽  
Burkholderia Cenocepacia ◽  
Burkholderia Cepacia Complex ◽  
Serum Samples ◽  
B Cell Epitopes ◽  
Life Threatening

Infections by the Burkholderia cepacia complex (Bcc) remain seriously life threatening to cystic fibrosis (CF) patients, and no effective eradication is available. A vaccine to protect patients against Bcc infections is a highly attractive therapeutic option, but none is available. A strategy combining the bioinformatics identification of putative surface-exposed proteins with an experimental approach encompassing the “shaving” of surface-exposed proteins with trypsin followed by peptide identification by liquid chromatography and mass spectrometry is here reported. The methodology allowed the bioinformatics identification of 263 potentially surface-exposed proteins, 16 of them also experimentally identified by the “shaving” approach. Of the proteins identified, 143 have a high probability of containing B-cell epitopes that are surface-exposed. The immunogenicity of three of these proteins was demonstrated using serum samples from Bcc-infected CF patients and Western blotting, validating the usefulness of this methodology in identifying potentially immunogenic surface-exposed proteins that might be used for the development of Bcc-protective vaccines.

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