Interstitial cells of Cajal and inflammation-induced motor dysfunction in the mouse small intestine

2000 ◽  
Vol 119 (6) ◽  
pp. 1590-1599 ◽  
Author(s):  
Tara Der ◽  
Premysl Bercik ◽  
Graeme Donnelly ◽  
Tim Jackson ◽  
Irene Berezin ◽  
...  
Keyword(s):  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Motor Dysfunction ◽  
Mouse Small Intestine ◽  
Cells Of Cajal

scholarly journals Effects of ATP on Pacemaker Activity of Interstitial Cells of Cajal from the Mouse Small Intestine

2018 ◽  
Vol 54 (1) ◽  
pp. 63
Author(s):  
Il Koo Park ◽  
Jin Ho Kim ◽  
Chan Guk Park ◽  
Man Yoo Kim ◽  
Shankar Prasad Parajuli ◽  
...  
Keyword(s):  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Pacemaker Activity ◽  
Mouse Small Intestine ◽  
Cells Of Cajal

scholarly journals Conditional genetic deletion of Ano1 in interstitial cells of Cajal impairs Ca2+ transients and slow waves in adult mouse small intestine

2017 ◽  
Vol 312 (3) ◽  
pp. G228-G245 ◽  
Author(s):  
John Malysz ◽  
Simon J. Gibbons ◽  
Siva A. Saravanaperumal ◽  
Peng Du ◽  
Seth T. Eisenman ◽  
...  
Keyword(s):  
Small Intestine ◽  
Slow Wave ◽  
Interstitial Cells Of Cajal ◽  
Adult Mouse ◽  
Rank Order ◽  
Interstitial Cells ◽  
Slow Waves ◽  
Functional Reserve ◽  
Mouse Small Intestine ◽  
Cells Of Cajal

Myenteric plexus interstitial cells of Cajal (ICC-MY) in the small intestine are Kit+ electrical pacemakers that express the Ano1/TMEM16A Ca2+-activated Cl– channel, whose functions in the gastrointestinal tract remain incompletely understood. In this study, an inducible Cre-LoxP-based approach was used to advance the understanding of Ano1 in ICC-MY of adult mouse small intestine. KitCreERT2/+;Ano1Fl/Fl mice were treated with tamoxifen or vehicle, and small intestines (mucosa free) were examined. Quantitative RT-PCR demonstrated ~50% reduction in Ano1 mRNA in intestines of conditional knockouts (cKOs) compared with vehicle-treated controls. Whole mount immunohistochemistry showed a mosaic/patchy pattern loss of Ano1 protein in ICC networks. Ca2+ transients in ICC-MY network of cKOs displayed reduced duration compared with highly synchronized controls and showed synchronized and desynchronized profiles. When matched, the rank order for Ano1 expression in Ca2+ signal imaged fields of view was as follows: vehicle controls>>>cKO(synchronized)>cKO(desynchronized). Maintenance of Ca2+ transients’ synchronicity despite high loss of Ano1 indicates a large functional reserve of Ano1 in the ICC-MY network. Slow waves in cKOs displayed reduced duration and increased inter-slow-wave interval and occurred in regular- and irregular-amplitude oscillating patterns. The latter activity suggested ongoing interaction by independent interacting oscillators. Lack of slow waves and depolarization, previously reported for neonatal constitutive knockouts, were also seen. In summary, Ano1 in adults regulates gastrointestinal function by determining Ca2+ transients and electrical activity depending on the level of Ano1 expression. Partial Ano1 loss results in Ca2+ transients and slow waves displaying reduced duration, while complete and widespread absence of Ano1 in ICC-MY causes lack of slow wave and desynchronized Ca2+ transients. NEW & NOTEWORTHY The Ca2+-activated Cl− channel, Ano1, in interstitial cells of Cajal (ICC) is necessary for normal gastrointestinal motility. We knocked out Ano1 to varying degrees in ICC of adult mice. Partial knockout of Ano1 shortened the widths of electrical slow waves and Ca2+ transients in myenteric ICC but Ca2+ transient synchronicity was preserved. Near-complete knockout was necessary for transient desynchronization and loss of slow waves, indicating a large functional reserve of Ano1 in ICC. View this article's corresponding video summary at https://youtu.be/cyPtDP0KLY4 .

T1802 Effects of Hydrogen Sulfide On Interstitial Cells of Cajal from Mouse Small Intestine

2009 ◽  
Vol 136 (5) ◽  
pp. A-583 ◽  
Author(s):  
Young Dae Kim ◽  
Jeong Kyun Seo ◽  
Chan-Guk Park ◽  
Man Woo Kim ◽  
Seok Choi ◽  
...  
Keyword(s):  
Small Intestine ◽  
Hydrogen Sulfide ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Mouse Small Intestine ◽  
Cells Of Cajal

scholarly journals Effects of sphingosine-1-phosphate on pacemaker activity of interstitial cells of Cajal from mouse small intestine

2013 ◽  
Vol 35 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Young Dae Kim ◽  
Kyoung Taek Han ◽  
Jun Lee ◽  
Chan Guk Park ◽  
Man Yoo Kim ◽  
...  
Keyword(s):  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Pacemaker Activity ◽  
Mouse Small Intestine ◽  
Sphingosine 1 Phosphate ◽  
Cells Of Cajal

Carbachol regulates pacemaker activities in cultured interstitial cells of Cajal from the mouse small intestine

2009 ◽  
Vol 27 (5) ◽  
pp. 525-531 ◽  
Author(s):  
Keum Young So ◽  
Sang Hun Kim ◽  
Hong Moon Sohn ◽  
Soo Jin Choi ◽  
Shankar Prasad Parajuli ◽  
...  
Keyword(s):  
Small Intestine ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Mouse Small Intestine ◽  
Cells Of Cajal
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