HIGH RESOLUTION INTERMODULATED AND DOUBLE RESONANCE ATOMIC SPECTROSCOPY IN A HOLLOW CATHODE

1983 ◽  
Vol 44 (C7) ◽  
pp. C7-217-C7-225 ◽  
Author(s):  
M. Inguscio
Keyword(s):  
High Resolution ◽  
Hollow Cathode ◽  
Double Resonance ◽  
Atomic Spectroscopy

scholarly journals High-resolution EPR distance measurements on RNA and DNA with the non-covalent Ǵ spin label

2019 ◽  
Vol 48 (2) ◽  
pp. 924-933 ◽  
Author(s):  
Marcel Heinz ◽  
Nicole Erlenbach ◽  
Lukas S Stelzl ◽  
Grace Thierolf ◽  
Nilesh R Kamble ◽  
...  
Keyword(s):  
High Resolution ◽  
Nucleic Acids ◽  
Spin Label ◽  
Double Resonance ◽  
Conformational Dynamics ◽  
Md Simulations ◽  
Spin Labels ◽  
Abasic Site ◽  
Abasic Sites ◽  
Rna And Dna

Abstract Pulsed electron paramagnetic resonance (EPR) experiments, among them most prominently pulsed electron-electron double resonance experiments (PELDOR/DEER), resolve the conformational dynamics of nucleic acids with high resolution. The wide application of these powerful experiments is limited by the synthetic complexity of some of the best-performing spin labels. The recently developed $\bf\acute{G}$ (G-spin) label, an isoindoline-nitroxide derivative of guanine, can be incorporated non-covalently into DNA and RNA duplexes via Watson-Crick base pairing in an abasic site. We used PELDOR and molecular dynamics (MD) simulations to characterize $\bf\acute{G}$, obtaining excellent agreement between experiments and time traces calculated from MD simulations of RNA and DNA double helices with explicitly modeled $\bf\acute{G}$ bound in two abasic sites. The MD simulations reveal stable hydrogen bonds between the spin labels and the paired cytosines. The abasic sites do not significantly perturb the helical structure. $\bf\acute{G}$ remains rigidly bound to helical RNA and DNA. The distance distributions between the two bound $\bf\acute{G}$ labels are not substantially broadened by spin-label motions in the abasic site and agree well between experiment and MD. $\bf\acute{G}$ and similar non-covalently attached spin labels promise high-quality distance and orientation information, also of complexes of nucleic acids and proteins.

scholarly journals Comparing the emission spectra of U and Th hollow cathode lamps and a new U line list

2018 ◽  
Vol 618 ◽  
pp. A118 ◽  
Author(s):  
L. F. Sarmiento ◽  
A. Reiners ◽  
P. Huke ◽  
F. F. Bauer ◽  
E. W. Guenter ◽  
...  
Keyword(s):  
Fourier Transform ◽  
High Resolution ◽  
Hollow Cathode ◽  
Line Intensity ◽  
Line Strength ◽  
Emission Spectra ◽  
Emission Lines ◽  
Line List ◽  
Line Intensity Ratio ◽  
Suitable Combination

Context. Thorium hollow cathode lamps (HCLs) are used as frequency calibrators for many high resolution astronomical spectrographs, some of which aim for Doppler precision at the 1 m s−1 level. Aims. We aim to determine the most suitable combination of elements (Th or U, Ar or Ne) for wavelength calibration of astronomical spectrographs, to characterize differences between similar HCLs, and to provide a new U line list. Methods. We record high resolution spectra of different HCLs using a Fourier transform spectrograph: (i) U–Ne, U–Ar, Th–Ne, and Th–Ar lamps in the spectral range from 500 to 1000 nm and U–Ne and U–Ar from 1000 to 1700 nm; (ii) we systematically compare the number of emission lines and the line intensity ratio for a set of 12 U–Ne HCLs; and (iii) we record a master spectrum of U–Ne to create a new U line list. Results. Uranium lamps show more lines suitable for calibration than Th lamps from 500 to 1000 nm. The filling gas of the lamps significantly affects their performance because Ar and Ne lines contaminate different spectral regions. We find differences (up to 88%) in the line intensity of U lines in different lamps from the same batch. We find 8239 isolated lines between 500 and 1700 nm that we attribute to U, 3379 of which were not contained in earlier line lists. Conclusions. We suggest using a combination of U–Ne and U–Ar lamps to wavelength-calibrate astronomical spectrographs up to 1 μm. From 1 to 1.7 μm, U–Ne shows better properties. The differences in line strength between different HCLs underline the importance of characterizing HCLs in the laboratory. The new 3379 U lines can significantly improve the radial velocity precision of astronomical spectrographs.

High resolution double‐resonance spectroscopy on Rydberg states of CO

1993 ◽  
Vol 99 (8) ◽  
pp. 5701-5711 ◽  
Author(s):  
Marcel Drabbels ◽  
Johannes Heinze ◽  
J. J. ter Meulen ◽  
W. Leo Meerts
Keyword(s):  
High Resolution ◽  
Double Resonance ◽  
Rydberg States ◽  
Resonance Spectroscopy ◽  
Double Resonance Spectroscopy

Rotational Analysis of the A2Π–X2Σ+ Band System of the BeBr Molecule

1975 ◽  
Vol 53 (14) ◽  
pp. 1321-1326 ◽  
Author(s):  
M. Carleer ◽  
M. Herman ◽  
R. Colin
Keyword(s):  
High Resolution ◽  
Hollow Cathode ◽  
Band System ◽  
Rotational Analysis ◽  
Molecular Constants ◽  
Bromine Vapor

A rotational analysis has been performed on the 0–0 band of the A2Π–X2Σ+ transition of the BeBr molecule photographed at high resolution in emission from a beryllium hollow cathode in the presence of bromine vapor. The following principal molecular constants have been determined:[Formula: see text]

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