scholarly journals Do trees use reserve or newly assimilated carbon for their defense reactions? A 13C labeling approach with young Scots pines inoculated with a bark-beetle-associated fungus (Ophiostoma brunneo ciliatum)

2007 ◽  
Vol 64 (6) ◽  
pp. 601-608 ◽  
Author(s):  
Natacha Guérard ◽  
Pascale Maillard ◽  
Claude Bréchet ◽  
François Lieutier ◽  
Erwin Dreyer
Keyword(s):  
Bark Beetle ◽  
13C Labeling ◽  
Defense Reactions ◽  
Labeling Approach ◽  
Ophiostoma Brunneo Ciliatum

Inter-tree variability in the induced defense reaction of Scots pine to single inoculations by Ophiostoma brunneo-ciliatum, a bark-beetle-associated fungus

1993 ◽  
Vol 59 (3-4) ◽  
pp. 257-270 ◽  
Author(s):  
François Lieutier ◽  
Jacques Garcia ◽  
Paul Romary ◽  
Annie Yart ◽  
Hervé Jactel ◽  
...  
Keyword(s):  
Scots Pine ◽  
Bark Beetle ◽  
Induced Defense ◽  
Defense Reaction ◽  
Ophiostoma Brunneo Ciliatum

Changes in phenolic metabolites of Scots-pine phloem induced by Ophiostoma brunneo-ciliatum, a bark-beetle-associated fungus

1996 ◽  
Vol 26 (3) ◽  
pp. 145-158 ◽  
Author(s):  
F. Lieutier ◽  
D. Sauvard ◽  
F. Brignolas ◽  
V. Picron ◽  
A. Yart ◽  
...  
Keyword(s):  
Scots Pine ◽  
Bark Beetle ◽  
Phenolic Metabolites ◽  
Ophiostoma Brunneo Ciliatum

scholarly journals Metabolic turnover and dynamics of modified ribonucleosides by 13C labeling

2021 ◽  
Author(s):  
Paulo A Gameiro ◽  
Vesela Encheva ◽  
Mariana Silva dos Santos ◽  
James I MacRae ◽  
Jernej Ule
Keyword(s):  
Small Rnas ◽  
Mammalian Cells ◽  
Tandem Mass ◽  
Dynamic Features ◽  
13C Labeling ◽  
Stationary Conditions ◽  
Base Modifications ◽  
Labeling Approach ◽  
Kinetics Of ◽  
Combined Measurements

Tandem mass spectrometry (MS/MS) is an accurate tool to assess modified ribonucleosides and their dynamics in mammalian cells. Yet, MS/MS quantification of lowly abundant modifications in non-ribosomal RNAs is unreliable, and the dynamic features of various modifications poorly understood. We developed a 13C labeling approach, 13C-dynamods, to quantify the turnover of base modifications in newly transcribed RNA. This turnover-based approach helped to resolve mRNA from ncRNA modifications in purified RNA or free ribonucleosides, and showed the distinct kinetics of N6-methyladenosine (m6A) versus 7-methylguanosine (m7G) in polyA+-purified RNA. We uncovered that N6,N6-dimethyladenosine (m62A) exhibits a distinct turnover in small RNAs and free ribonucleosides when compared to the known m62A-modified large rRNAs. Finally, combined measurements of turnover and abundance informed on the transcriptional versus posttranscriptional sensitivity of modified ncRNAs and mRNAs, respectively, to stress conditions. Thus, 13C-dynamods enables studies of origin of modified RNAs at steady-state and their dynamics under non-stationary conditions.

Conifer snagfall data in bark-beetle infested subalpine forests at the Fraser Experimental Forest, Colorado, USA

2019 ◽  
Author(s):  
Charles C. Rhoades ◽  
Robert M. Hubbard ◽  
Paul R. Hood ◽  
Banning J. Starr ◽  
Daniel B. Tinker ◽  
...  
Keyword(s):  
Bark Beetle ◽  
Subalpine Forests
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