scholarly journals Population genetics of Norway spruce (Picea abiesKarst.) at regional scale: sensitivity of different microsatellite motif classes in detecting differentiation

2006 ◽  
Vol 63 (5) ◽  
pp. 485-491 ◽  
Author(s):  
Ivan Scotti ◽  
Gianpaolo Paglia ◽  
Federica Magni ◽  
Michele Morgante
1992 ◽  
Vol 22 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Anders Kvarnheden ◽  
Peter Engström

DNA fingerprinting techniques have significantly improved the resolution of the analysis of genetic polymorphisms in major eukaryotic taxa. The techniques are based on the use of specific DNA probes, which hybridize to families of related minisatellite loci that are dispersed in the genomes of a range of eukaryotes. These sequences are highly variable as a result of a variation in the numbers of a core repeat sequence at each locus. We wanted to establish whether one such probe, the DNA of the bacteriophage M13, could be used to detect hypervariable loci in the conifer Norway spruce, Piceaabies (L.) Karst., and to examine if the method could detect genetic differences at the level of populations and (or) individual trees. The results show that hypervariable minisatellite sequences that hybridize to the M13 probe are present in Norway spruce. The minisatellite sequences are stably inherited, and the variability within the species is sufficiently high to allow the distinction of different individuals. The differences between populations are of the same order of magnitude as those between trees within populations. The method is potentially useful in population genetics research on conifers, as well as in breeding programs.


2015 ◽  
Vol 106 (S1) ◽  
pp. 503-511 ◽  
Author(s):  
Fernanda Pedone Valdez ◽  
Taiana Haag ◽  
Fernando C. C. Azevedo ◽  
Leandro Silveira ◽  
Sandra M. C. Cavalcanti ◽  
...  

2017 ◽  
Vol 405 ◽  
pp. 350-359 ◽  
Author(s):  
Erica A. Di Pierro ◽  
Elena Mosca ◽  
Santiago C. González-Martínez ◽  
Giorgio Binelli ◽  
David B. Neale ◽  
...  

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jorge García-Girón ◽  
Pedro García ◽  
Margarita Fernández-Aláez ◽  
Eloy Bécares ◽  
Camino Fernández-Aláez

AbstractThe degree to which dispersal limitation interacts with environmental filtering has intrigued metacommunity ecologists and molecular biogeographers since the beginning of both research disciplines. Since genetic methods are superior to coarse proxies of dispersal, understanding how environmental and geographic factors influence population genetic structure is becoming a fundamental issue for population genetics and also one of the most challenging avenues for metacommunity ecology. In this study of the aquatic macrophyte Myriophyllum alterniflorum DC., we explored the spatial genetic variation of eleven populations from the Iberian Plateau by means of microsatellite loci, and examined if the results obtained through genetic methods match modern perspectives of metacommunity theory. To do this, we applied a combination of robust statistical routines including network analysis, causal modelling and multiple matrix regression with randomization. Our findings revealed that macrophyte populations clustered into genetic groups that mirrored their geographic distributions. Importantly, we found a significant correlation between genetic variation and geographic distance at the regional scale. By using effective (genetic) dispersal estimates, our results are broadly in line with recent findings from metacommunity theory and re-emphasize the need to go beyond the historically predominant paradigm of understanding environmental heterogeneity as the main force driving macrophyte diversity patterns.


2008 ◽  
Vol 57 (1-6) ◽  
pp. 41-44 ◽  
Author(s):  
F. Maghuly ◽  
K. Burg ◽  
W. Pinsker ◽  
F. Nittinger ◽  
W. Praznik ◽  
...  

AbstractNorway spruce is an important commercial tree species in northern and central Europe. Pure mitochondrial DNA isolated from tissue culture materials grown in the dark were used to construct a partial mitochondrial library. 100 clones were randomly selected and 19 markers were isolated. Three of these markers proved to be polymorphic and two showed maternal inheritance in controlled crosses. These markers will be useful for population genetic studies in P. abies.


Forests ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 34
Author(s):  
Maja Bryk ◽  
Beata Kołodziej ◽  
Radosław Pliszka

Among the largest disturbances affecting the health of spruce forests is the large-scale appearance of bark beetles. Knowledge on the spatial distribution of infected-spruce areas is vital for effective and sustainable forest management. Medium-spatial-resolution (20–30 m) satellite images are well-suited for spruce forest disturbance monitoring at a landscape and regional scale following bark beetle outbreaks. The aim of this study was to evaluate the health of a Norway spruce stand after a bark beetle outbreak based on Landsat 8 images and thematic and vector data, supplemented with selected climate variables. This research was conducted for a spruce stand in the Białowieża Forest District in 2013, 2015, 2017, and 2019. We hypothesised that the changes in spruce health would significantly influence the NDVI distributions during the studied years. Our research revealed that the weather conditions in the period of May–September were beneficial for beetle development and detrimental for the spruce stand, particularly in 2015, 2018, and 2019. SWIR-NIR-G and NDVI images showed a gradual deterioration in spruce health. The quantitative NDVI distributions varied; the minimum, mean, and median decreased; and the distribution shape of the index values changed over the studied years. An analysis of the spatial NDVI distributions revealed that the threshold NDVI value separating spruce stand areas in good and poor health was ca. 0.6. This study confirmed the applicability of NDVI for monitoring alterations in spruce stands, and indicated that spatial NDVI distributions can provide valuable support in forest monitoring at a landscape scale, since medium-resolution, ready-to-use NDVI images are easily available from the Landsat archives, facilitating the routine assessment of stand health.


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