scholarly journals Killer yeasts as biocontrol agents of spoilage yeasts and bacteria isolated from wine

2014 ◽  
Vol 3 ◽  
pp. 02001
Author(s):  
Miguel Fernández de Ullivarri ◽  
Lucía M. Mendoza ◽  
Raúl R. Raya
Fermentation ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 53
Author(s):  
Maxwell Mewa-Ngongang ◽  
Heinrich W. du Plessis ◽  
Edwin Hlangwani ◽  
Seteno K. O. Ntwampe ◽  
Boredi S. Chidi ◽  
...  

It is common to find different spoilage organisms occurring in the same food item, which usually requires food producers to utilize a mixture of synthetic preservatives to control spoilage. This study evaluated the interaction between mixtures of crude biopreservatives against consortia of common spoilage yeasts occurring in beverages. Crude biopreservatives produced from separate yeasts were formulated in different growth inhibition combinations (GICs), i.e., GIC1 (Candida pyralidae Y1117 and Pichia kluyveri Y1125), GIC 2 (C. pyralidae Y1117 and P. kluyveri Y1164), GIC3 (P. kluyveri Y1125 and P. kluyveri Y1164), and GIC4 (C. pyralidae, P. kluyveri Y1125 and P. kluyveri Y1164). The spoilage yeast consortia combinations, i.e., SC1 (Dekkera. anomala and D. bruxellensis), SC2 (D. anomala and Zygosaccharomyces bailii), SC3 (D. bruxellensis and Z. bailii), and SC4 (D. anomala, D. bruxellensis and Z. bailii), were also prepared. The highest growth inhibition activities of the crude biopreservatives were observed at a pH of 3.0 and 2.0 for C. pyralidae and P. kluyveri strains, respectively, while reduced activity was observed at a pH of 4.0 and 5.0. The growth inhibition proficiency depended on the spoilage yeast or the consortia of spoilage yeasts. Biocontrol agents from an individual yeast or mixtures can be used to prevent food and beverage spoilage.


Author(s):  
María Florencia Perez ◽  
Ana Sofía Isas ◽  
Azzam Aladdin ◽  
Hesham A. El Enshasy ◽  
Julián Rafael Dib

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0165590 ◽  
Author(s):  
María Florencia Perez ◽  
Luciana Contreras ◽  
Nydia Mercedes Garnica ◽  
María Verónica Fernández-Zenoff ◽  
María Eugenia Farías ◽  
...  

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
P Avato ◽  
M Argentieri ◽  
S Laquale ◽  
T D'Addabbo
Keyword(s):  

2020 ◽  
pp. 1-8
Author(s):  
Beilei Wu ◽  
Beilei Wu ◽  
Mei Li ◽  
Xiaoli Chen ◽  
Xiliang Jiang ◽  
...  

Endophytes are increasingly investigated as biocontrol agents for agricultural production. The identification of new endophytes with high effectiveness against plant disease is very important. A total of 362 strains of endophytes, including fungi, bacteria, and actinomycete, were isolated from alfalfa (Medicago sativa L.) collected in Hebei, Inner Mongolia and Ningxia provinces of China. The three strains of endophytic bacteria (NA NX51R-5, NA NX90R-8, and NA NM1S-1) with strong biocontrol capability with >50% effectiveness were screened against the common alfalfa root rot pathogen Fusarium oxysporum F. sp. medicaginis in alfalfa seedling germination experiments on MS medium and pot experiments. Using phylogenetic analysis, the isolates of NA NM1S-1 and NA NX51R-5 were identified as Bacillus spp. by 16S rDNA, while NA NX90R-8 was found to be Pseudomonas sp.


Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 664
Author(s):  
Matías Olivera ◽  
Ninoska Delgado ◽  
Fabiola Cádiz ◽  
Natalia Riquelme ◽  
Iván Montenegro ◽  
...  

Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms isolated from table grapes and identified as Hanseniaspora osmophila and Gluconobacter cerinus was evaluated. In dual cultures, both biocontrol agents (together and separately) inhibited in vitro mycelial growth of these pathogens. To identify the compounds responsible for the inhibitory effect, extractions were carried out with organic solvents from biocontrol agents separately. Through dual cultures with pathogens and pure extracts, only the hexane extract from H. osmophila showed an inhibitory effect against Botrytis cinerea. To further identify these compounds, the direct bioautography technique was used. This technique made it possible to determine the band displaying antifungal activity at Rf = 0.05–0.2. The compounds present in this band were identified by GC-MS and compared to the NIST library. The most abundant compounds, not previously reported, corresponded to alkanes, ketones, alcohols, and terpenoids. H. osmophila and G. cerinus have the potential to control the causal agents of gray and summer bunch rot of table grapes.


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