scholarly journals The use of oligonucleotide probes to study the ecology of ruminal microbial populations

1996 ◽  
Vol 45 (Suppl. 1) ◽  
pp. 281-285 ◽  
Author(s):  
RI Mackie
Keyword(s):  
Microbial Populations ◽  
Oligonucleotide Probes ◽  
Ruminal Microbial Populations

Postprandial variations in the activity of polysaccharide-degrading enzymes of fluid- and particle-associated ruminal microbial populations

1993 ◽  
Vol 27 (4) ◽  
pp. 223-228 ◽  
Author(s):  
Cécile Martin ◽  
Brigitte Michalet-Doreau ◽  
Gérard Fonty ◽  
Alan Williams
Keyword(s):  
Microbial Populations ◽  
Degrading Enzymes ◽  
Ruminal Microbial Populations

scholarly journals Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

1990 ◽  
Vol 56 (6) ◽  
pp. 1919-1925 ◽  
Author(s):  
R I Amann ◽  
B J Binder ◽  
R J Olson ◽  
S W Chisholm ◽  
R Devereux ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
16S Rrna ◽  
Microbial Populations ◽  
Oligonucleotide Probes

scholarly journals Quantitative Fluorescence In Situ Hybridization of Microbial Communities in the Rumens of Cattle Fed Different Diets

2010 ◽  
Vol 76 (20) ◽  
pp. 6933-6938 ◽  
Author(s):  
Yunhong Kong ◽  
Maolong He ◽  
Tim McAlister ◽  
Robert Seviour ◽  
Robert Forster
Keyword(s):  
Quantitative Information ◽  
Total Cell ◽  
Microbial Populations ◽  
Fibrobacter Succinogenes ◽  
Oligonucleotide Probes ◽  
Bacterial Populations ◽  
Cell Numbers ◽  
Barley Silage ◽  
Quantitative Fluorescence

ABSTRACT At present there is little quantitative information on the identity and composition of bacterial populations in the rumen microbial community. Quantitative fluorescence in situ hybridization using newly designed oligonucleotide probes was applied to identify the microbial populations in liquid and solid fractions of rumen digesta from cows fed barley silage or grass hay diets with or without flaxseed. Bacteroidetes, Firmicutes, and Proteobacteria were abundant in both fractions, constituting 31.8 to 87.3% of the total cell numbers. They belong mainly to the order Bacteroidales (0.1 to 19.2%), hybridizing with probe BAC1080; the families Lachnospiraceae (9.3 to 25.5%) and Ruminococcaceae (5.5 to 23.8%), hybridizing with LAC435 and RUM831, respectively; and the classes Deltaproteobacteria (5.8 to 28.3%) and Gammaproteobacteria (1.2 to 8.2%). All were more abundant in the rumen communities of cows fed diets containing silage (75.2 to 87.3%) than in those of cows fed diets containing hay (31.8 to 49.5%). The addition of flaxseed reduced their abundance in the rumens of cows fed silage-based diets (to 45.2 to 58.7%) but did not change markedly their abundance in the rumens of cows fed hay-based diets (31.8 to 49.5%). Fibrolytic species, including Fibrobacter succinogenes and Ruminococcus spp., and archaeal methanogens accounted for only a small proportion (0.4 to 2.1% and 0.2 to 0.6%, respectively) of total cell numbers. Depending on diet, between 37.0 and 91.6% of microbial cells specifically hybridized with the probes used in this study, allowing them to be identified in situ. The identities of other microbial populations (8.4 to 63.0%) remain unknown.

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