scholarly journals Measurements of microbial N flow to the duodenum and urinary excretion of purine derivatives in bulls

1995 ◽  
Vol 44 (Suppl. 1) ◽  
pp. 177-177 ◽  
Author(s):  
Y. Beckers ◽  
A. Théwis ◽  
C. Sohy ◽  
E. François
Keyword(s):  
Urinary Excretion ◽  
Purine Derivatives ◽  
Microbial N

The relationship between microbial N synthesis and urinary excretion of purine derivatives in Dorper×thin-tailed Han crossbred sheep

2013 ◽  
Vol 112 (1-3) ◽  
pp. 49-55 ◽  
Author(s):  
Tao Ma ◽  
Kaidong Deng ◽  
Chenggang Jiang ◽  
Yan Tu ◽  
Naifeng Zhang ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Purine Derivatives ◽  
The Relationship ◽  
Microbial N

Urinary recovery of allantoin in normally fed steers

1998 ◽  
Vol 1998 ◽  
pp. 80-80
Author(s):  
F. Herrera Gomez ◽  
F. D. Deb Hovell ◽  
C. A. Sandoval Castro
Keyword(s):  
Urinary Excretion ◽  
Recovery Factor ◽  
Purine Derivatives ◽  
Urinary Recovery ◽  
Host Animal ◽  
N Supply ◽  
Normal Feeding ◽  
The Relationship ◽  
Infusion Technique ◽  
Microbial N

Studies in the use of the purine derivatives technique in ruminants have been stimulated by the possible use of this technique as an estimator of the rumen microbial-N supplied to the host animal. The recovery factor influences the estimation of the total purines absorbed and therefore the microbial-N supply. The relationship between exogenous purine input and urinary excretion and recovery has been studied using cattle maintained with the intragastric infusion technique (Orskov et al., 1979). The urinary recovery of exogenous purines has been estimated to be 0.77-0.85 (Chen et al., 1990a, Verbic et al., 1990), and this relationship has been assumed to be applicable to normal feeding situations. To our knowledge there is no data to support or reject this approach. This study examined the urinary recovery of exogenous allantoin input in steers under normal feeding conditions.

Urinary recovery of allantoin in normally fed steers

1998 ◽  
Vol 1998 ◽  
pp. 80-80
Author(s):  
F. Herrera Gomez ◽  
F. D. Deb Hovell ◽  
C. A. Sandoval Castro
Keyword(s):  
Urinary Excretion ◽  
Recovery Factor ◽  
Purine Derivatives ◽  
Urinary Recovery ◽  
Host Animal ◽  
N Supply ◽  
Normal Feeding ◽  
The Relationship ◽  
Infusion Technique ◽  
Microbial N

Studies in the use of the purine derivatives technique in ruminants have been stimulated by the possible use of this technique as an estimator of the rumen microbial-N supplied to the host animal. The recovery factor influences the estimation of the total purines absorbed and therefore the microbial-N supply. The relationship between exogenous purine input and urinary excretion and recovery has been studied using cattle maintained with the intragastric infusion technique (Orskov et al., 1979). The urinary recovery of exogenous purines has been estimated to be 0.77-0.85 (Chen et al., 1990a, Verbic et al., 1990), and this relationship has been assumed to be applicable to normal feeding situations. To our knowledge there is no data to support or reject this approach. This study examined the urinary recovery of exogenous allantoin input in steers under normal feeding conditions.

scholarly journals Determination of rumen microbial-nitrogen production in sheep: a comparison of urinary purine excretion with methods using 15N and purine bases as markers of microbial-nitrogen entering the duodenum

1996 ◽  
Vol 75 (5) ◽  
pp. 699-709 ◽  
Author(s):  
J. F. erez ◽  
J. Balcells ◽  
J. A. Guada ◽  
C. Castrillo
Keyword(s):  
Urinary Excretion ◽  
Basal Diet ◽  
Barley Grain ◽  
Rumen Content ◽  
Purine Derivatives ◽  
Flow Measurements ◽  
Mean Values ◽  
Purine Bases ◽  
Microbial Nitrogen ◽  
Microbial N

The present study compares estimates of rumen microbial-N production derived from duodenal flow measurements (15N and purine bases) with those from measurements of the urinary excretion of purine derivatives. Four Rasa Aragonesa ewes fitted with simple cannulas in the rumen and proximal duodenum were used. Four diets consisting of 550 g lucerne (Medicago sativa) hay/d as sole feed or supplemented with 220, 400 and 550 g rolled barley grain/d were given in a 4 x 4 random factorial arrangement. Duodenal digesta flows were determined by the dual-phase marker technique during continuous intraruminal infusions of Co-EDTA and Yb-acetate. Microbial contribution to the non-NH3N (NAN)flow was estimated from 15N enrichment and purines: N ratio in duodenal digesta and bacterial fractions isolated from the rumen content. Whole tract organic matter (OM) digestibility and duodenal flow of OM and NAN increased (P<0·001) with the level of barley supplementation. Digestible OM intake ranged from 19·0 to 42·7 g/kg metabolic weight (W0·75) and the duodenal flow of purine bases and the urinary excretion of allantoin increased Linearly (P < 0·001) from minimum values of 7·47 (SD 1·524)and 4·65 (SD 0·705) mmol/d respectively on the basal diet to 18·20 (SD 1·751) and 11·62 (SD 0·214) mmol/d on the 400 g barley diet; a further increase in barley supplementation decreased both variables (13/50 (SD 2/334) and 8/77 (SD 0/617) mmol/d respectively). Urinary excretion of uric acid and hypoxanthine showed a slight but significant increase (P < 0·05) over all levels of barley. Molar recoveries of duodenal purine bases as purine derivatives or allantoin in the urine were 0·78 (SD 0·156) and 0·65 (SD 0·130) respectively. The increase on barley supplementation significantly augmented microbial-N, but large differences between microbial markers employed were observed. Mean values of microbial-N estimated from the duodenal purine bases or urinary allantoin excretion were on average 18 and 29% lower than those measured by 15N.

Urinary purine derivatives excretion in Bos taurus and Bos indicus cattle

1998 ◽  
Vol 1998 ◽  
pp. 23-23
Author(s):  
F. Herrera Gomez ◽  
F.D.DeB Hovell ◽  
C.A. Sandoval Castro
Keyword(s):  
Small Intestine ◽  
Urinary Excretion ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Purine Derivatives ◽  
Non Invasive ◽  
N Supply ◽  
Tropical Conditions ◽  
Invasive Method ◽  
Microbial N

The purine derivatives (PD) have been proposed as a non-invasive method to estimate microbial-N supply to the small intestine (Chen et al., 1990a; Verbic et al., 1990). The use of PD urinary excretion has the advantage that it can be used with intact animals thus reducing the concern of animal welfare issues. Although, there are known differences in purine metabolism between cattle (B. taurus), sheep and buffaloes (Bubalis bubalis) (Chen et al., 1990b; Chen et al., 1996), no direct comparison of PD urinary excretion has been made so far between cattle especies, therefore, the objective of the present experiment was to compare PD urinary excretion of B. taurus and B. indicus cattle fed similar diets under tropical conditions.

Rumen microbial production estimated either from urinary purine derivative excretion or from direct measurements of 15N and purine bases as microbial markers: effect of protein source and rumen bacteria isolates

1997 ◽  
Vol 65 (2) ◽  
pp. 225-236 ◽  
Author(s):  
J. F. Pérez ◽  
J. Balcells ◽  
J. A. Guada ◽  
C. Castrillo
Keyword(s):  
Organic Matter ◽  
Urinary Excretion ◽  
Fish Meal ◽  
Soya Bean ◽  
Purine Derivatives ◽  
Sunflower Meal ◽  
Purine Bases ◽  
Direct Measurements ◽  
Digestible Organic Matter ◽  
Microbial N

AbstractFour ewes fitted with ruminal and duodenal T-piece cannulae were each given six diets in a 6 × 4 factorial design. Diets or experimental treatments consisted of two ratios of forage: concentrate (700:150 (LC) and 400: 600 (HO). Forage was ammonia-treated straw and the concentrate was formulated with barley supplemented with one of three protein sources: sunflower meal, soya-bean meal or fish meal. Duodenal flows ofdigesta were estimated by the dual-phase technique using Co-EDTA and Yb acetate as liquid and solid markers. Microbial nitrogen (N) was estimated from the digesta flow of purine bases and 15N enrichment using as reference samples, bacterial isolates from the liquid (LAB) or solid (SAB) phase of rumen digesta.Duodenal flow of purine bases (mmol/day) was lower on LC (12·9) than HC (17·7) diets but in both treatments it was depressed by fish meal (12·3) compared with either soya-bean (17·3) or sunflower meal (16·3) as supplements (s.e. 1·13). Urinary excretion of purine derivatives showed a similar trend, 8·6 v. III mmol/day in LC and HC respectively and 8·8 v. 10·4 and 10·5 mmol/day in fish meal, soya-bean and sunflower meal diets (s.e. 0·56), respectively. Variation in excretion of urinary purine derivatives was mainly associated with digestible organic matter intake with an average ratio of 1·7 (s.e. 0·11) mmol per 100 g digestible organic matter intake. Irrespective of the microbial marker used, microbial yield was higher in animals offered HC than in those offered LC and with soya-bean or sunflower meal compared with fish meal supplemented diets. The microbial purine bases/N (mmol/g) ratio varied between LAB (1·99, s.e. 0·092) and SAB (1·69, s.e. 0·071) isolates leading to different estimates of microbial-N yield (g) from duodenal purine bases (7·76 (s.e. 2·84) v. 9·13 (s.e. 3·24)), urinary excretion of allantoin (5·57 (s.e. 2·0) v. 6·57 (s.e. 2·03)) or total purine derivatives (6·43 (s.e. 2·39) v. 7·56 (s.e. 2·77)). Urinary excretion of allantoin or total purine derivatives provided consistently lower estimates of duodenal microbial-N than duodenal purine bases or 15N, although it closely reflected the pattern observed in direct measurements.

Urinary purine derivatives excretion in Bos taurus and Bos indicus cattle

1998 ◽  
Vol 1998 ◽  
pp. 23-23
Author(s):  
F. Herrera Gomez ◽  
F.D.DeB Hovell ◽  
C.A. Sandoval Castro
Keyword(s):  
Small Intestine ◽  
Urinary Excretion ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Purine Derivatives ◽  
Non Invasive ◽  
N Supply ◽  
Tropical Conditions ◽  
Invasive Method ◽  
Microbial N

The purine derivatives (PD) have been proposed as a non-invasive method to estimate microbial-N supply to the small intestine (Chen et al., 1990a; Verbic et al., 1990). The use of PD urinary excretion has the advantage that it can be used with intact animals thus reducing the concern of animal welfare issues. Although, there are known differences in purine metabolism between cattle (B. taurus), sheep and buffaloes (Bubalis bubalis) (Chen et al., 1990b; Chen et al., 1996), no direct comparison of PD urinary excretion has been made so far between cattle especies, therefore, the objective of the present experiment was to compare PD urinary excretion of B. taurus and B. indicus cattle fed similar diets under tropical conditions.

scholarly journals Measurements of microbial N flow to the duodenum and urinary excretion of purine derivatives in bulls

1995 ◽  
Vol 44 (Suppl. 1) ◽  
pp. 177-177
Author(s):  
Y. Beckers ◽  
A. Théwis ◽  
C. Sohy ◽  
E. François
Keyword(s):  
Urinary Excretion ◽  
Purine Derivatives ◽  
Microbial N

The effect of rumen protozoa on the urinary excretion of purine derivatives in goats

2003 ◽  
Vol 140 (1) ◽  
pp. 101-105 ◽  
Author(s):  
T. FUJIHARA ◽  
M. TODOROKI ◽  
K. NAKAMURA
Keyword(s):  
Urinary Excretion ◽  
N Balance ◽  
Purine Derivatives ◽  
Purine Derivative ◽  
Rumen Protozoa ◽  
The Mean

Urinary purine derivative (PD) excretion was estimated to examine the effect of rumen protozoa on total PD excretion in goats fed hay and a concentrate diet. The effect of increasing protozoa number in the rumen on nitrogen (N) balance and urinary PD excretion was determined after inoculation. Protozoa increased slowly until 4 days after inoculation, and on the 5th day after inoculation rapidly, finally (10 days) reaching 4·1×105/ml of rumen contents similar to that before defaunation. Urinary N excretion showed a small (non-significant) decrease. Urinary PD excretion did not change until the 7th day, and then the level decreased on the 8th day after faunation presumably due to the effect of increased protozoa in the rumen. The mean urinary total PD excretion significantly (P<0·05) decreased in the defaunated group compared with that in the faunated group. Comparable changes were not seen in plasma PD level of faunated and defaunated groups.

Purine derivatives in spot urine samples and plasma of sheep: a possible index of microbial protein supply

1993 ◽  
Vol 1993 ◽  
pp. 5-5 ◽  
Author(s):  
X. B. Chen ◽  
Adriana T. Mejia ◽  
D. J. Kyle ◽  
E. R. Ørskov
Keyword(s):  
Urinary Excretion ◽  
Feed Intake ◽  
Urine Samples ◽  
Plasma Samples ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Feeding Regime ◽  
Spot Urine ◽  
Alternative Index ◽  
Protein Supply

In ruminants, daily urinary excretion of purine derivatives (PD) reflects the absorption of microbial purines and can be used as an index of microbial protein supply (Chen, Ørskov and Hovell, 1991). The application could be extended to farm conditions if measurements based on spot urine samples or plasma could serve as an alternative index. The objective of this study was to examine whether PD concentrations in spot urine or plasma samples vary diurnally during a given feeding regime and if they reflect differences in daily PD excretion induced by varying feed intake.

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