scholarly journals Bread wheat x Agrotricum crosses as a source of immunity and resistance to the PAV strain of barley yellow dwarf luteovirus

Agronomie ◽  
1994 ◽  
Vol 14 (3) ◽  
pp. 153-160 ◽  
Author(s):  
A. Comeau ◽  
KM Makkouk ◽  
F. Ahmad ◽  
CA Saint-Pierre
Keyword(s):  
Bread Wheat ◽  
Yellow Dwarf ◽  
Barley Yellow Dwarf ◽  
Barley Yellow Dwarf Luteovirus

scholarly journals Resistance to barley yellow dwarf luteovirus in Aegilops species

1994 ◽  
Vol 74 (3) ◽  
pp. 631-634 ◽  
Author(s):  
K. M. Makkouk ◽  
W. Ghulam ◽  
A. Comeau
Keyword(s):  
Triticum Aestivum ◽  
Agricultural Research ◽  
Sandwich Elisa ◽  
Yellow Dwarf ◽  
Aegilops Species ◽  
Elite Group ◽  
Barley Yellow Dwarf ◽  
Tissue Blot Immunoassay ◽  
Das Elisa ◽  
Barley Yellow Dwarf Luteovirus

One thousand and ninety-seven Aegilops accessions were evaluated for their reaction to a PAV serotype of barley yellow dwarf luteovirus (BYDV). The accessions tested belong to the species bicornis, biuncialis, caudata, crassa, columnaris, comosa, cylindrica, kotschyi, longissima, mutica, neglecta (= triaristata 4 ×), ovata, peregrina, searsii, sharonensis, speltoides, tauschii (= squarrosa), triuncialis, umbellulata, uniaristata, vavilovii and ventricosa. The first evaluation of virus levels in the different accessions was conducted at International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria, using double antibody sandwich ELISA (DAS-ELISA). Accession reaction ranged from highly resistant to highly susceptible. Thirty-eight Aegilops accessions resistant at ICARDA, were evaluated at Sainte-Foy, Quebec, Canada, by tissue-blot immunoassay. Diversity of response to BYDV infection was again observed in this elite group. Seven accessions belonging to the species biuncialis, caudata, neglecta and triuncialis were highly BYDV resistant at both locations; five of these originated from Bulgaria. Key words: Introgression, interspecific, Triticum aestivum, BYDV, ELISA, immunoassay, tissue blot

scholarly journals Expression of Thinopyrum intermedium-Derived Barley yellow dwarf virus Resistance in Elite Bread Wheat Backgrounds

2001 ◽  
Vol 91 (1) ◽  
pp. 55-62 ◽  
Author(s):  
L. Ayala ◽  
M. van Ginkel ◽  
M. Khairallah ◽  
B. Keller ◽  
M. Henry
Keyword(s):  
Bread Wheat ◽  
Seed Quality ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thinopyrum Intermedium ◽  
Mendelian Segregation ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

Resistance to Barley yellow dwarf virus (BYDV) is not found in wheat but is available in a Thinopyrum intermedium translocation (Ti) carried on chromosome 7DL of bread wheat recombinant lines. We used one of those lines (TC14/2*Spear) to introgress the Ti into bread wheat cultivars and to determine the influence of wheat backgrounds, with and without known tolerance to BYDV, on the expression of resistance. Two single and three backcross populations, segregating for the presence of the alien fragment, were tested under field conditions and artificial inoculation with BYDV isolates MAV-Mex and PAV-Mex. Lines containing the fragment were identified using the microsatellite marker gwm37. Tillering, biomass, grain yield, thousand-kernel weight, and seed quality were evaluated in inoculated and noninoculated plots. Resistance was assessed by enzyme-linked immunosorbent assay. In early generations, the alien fragment followed expected Mendelian segregation, whereas in the advanced ones a slight bias against its transmission was observed. No positive nor negative effects of Ti on agronomic performance and quality were found. A significant optical density reduction in individuals carrying the fragment was observed after PAV infection in crosses with lines Anza and Baviacora but not with Milan. In addition, the fragment was associated with a lower frequency of infected plants for both PAV and MAV isolates. The reduced yield loss associated with the presence of the translocation was due largely to the lower infection rate.

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