scholarly journals Sensibilité différente de deux populations de Spodoptera exigua Hüb. (Lépid., Noctuidae) aux baculovirus et aux pyréthrinoïdes de synthèse

Agronomie ◽  
1986 ◽  
Vol 6 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Josette CHAUFAUX ◽  
Pierre FERRON
Keyword(s):  
Spodoptera Exigua ◽  
Pyrethrinoïdes De Synthèse

scholarly journals Bacmid Expression of Granulovirus Enhancin En3 Accumulates in Cell Soluble Fraction to Potentiate Nucleopolyhedrovirus Infection

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1233
Author(s):  
Adriana Ricarte-Bermejo ◽  
Oihane Simón ◽  
Ana Beatriz Fernández ◽  
Trevor Williams ◽  
Primitivo Caballero
Keyword(s):  
Trichoplusia Ni ◽  
Recombinant Virus ◽  
Spodoptera Exigua ◽  
Insect Cells ◽  
Soluble Fraction ◽  
Autographa Californica ◽  
Insect Midgut ◽  
Baculovirus Infection ◽  
Occlusion Bodies ◽  
Infected Cells

Enhancins are metalloproteinases that facilitate baculovirus infection in the insect midgut. They are more prevalent in granuloviruses (GVs), constituting up to 5% of the proteins of viral occlusion bodies (OBs). In nucleopolyhedroviruses (NPVs), in contrast, they are present in the envelope of the occlusion-derived virions (ODV). In the present study, we constructed a recombinant Autographa californica NPV (AcMNPV) that expressed the Trichoplusia ni GV (TnGV) enhancin 3 (En3), with the aim of increasing the presence of enhancin in the OBs or ODVs. En3 was successfully produced but did not localize to the OBs or the ODVs and accumulated in the soluble fraction of infected cells. As a result, increased OB pathogenicity was observed when OBs were administered in mixtures with the soluble fraction of infected cells. The mixture of OBs and the soluble fraction of Sf9 cells infected with BacPhEn3 recombinant virus was ~3- and ~4.7-fold more pathogenic than BacPh control OBs in the second and fourth instars of Spodoptera exigua, respectively. In contrast, when purified, recombinant BacPhEn3 OBs were as pathogenic as control BacPh OBs. The expression of En3 in the soluble fraction of insect cells may find applications in the development of virus-based insecticides with increased efficacy.

Toxicity of Pyrrolizidine Alkaloids to Spodoptera exigua Using Insect Cell Lines and Injection Bioassays

2014 ◽  
Vol 40 (6) ◽  
pp. 609-616 ◽  
Author(s):  
Tri R. Nuringtyas ◽  
Robert Verpoorte ◽  
Peter G. L. Klinkhamer ◽  
Monique M. van Oers ◽  
Kirsten A. Leiss
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Pyrrolizidine Alkaloids ◽  
Spodoptera Exigua ◽  
Insect Cell Lines
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