Deregulation of BCL2 family genes in glioblastoma cells consequent to poly(butyl cyanoacrylate) nanoparticles treatment

2019 ◽  
Vol 14 (10) ◽  
pp. 1102-1106
Author(s):  
Mahdieh Sadat Taghavi ◽  
Azim Akbarzadeh ◽  
Reza Mahdian
Keyword(s):  
Glioblastoma Cells ◽  
Bcl2 Family

Glioblastoma invasion and NMDA receptors: A novel prospect

2019 ◽  
Vol 106 (3) ◽  
pp. 250-260 ◽  
Author(s):  
DN Nandakumar ◽  
P Ramaswamy ◽  
C Prasad ◽  
D Srinivas ◽  
K Goswami
Keyword(s):  
Glutamate Receptors ◽  
Cell Lines ◽  
Intracellular Signaling ◽  
Transcript Level ◽  
Glioblastoma Cells ◽  
Invasion And Migration ◽  
Matrigel Invasion ◽  
Nmdar Activation ◽  
And Migration

Purpose Glioblastoma cells create glutamate-rich tumor microenvironment, which initiates activation of ion channels and modulates downstream intracellular signaling. N-methyl-D-aspartate receptors (NMDARs; a type of glutamate receptors) have a high affinity for glutamate. The role of NMDAR activation on invasion of glioblastoma cells and the crosstalk with α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) is yet to be explored. Main methods LN18, U251MG, and patient-derived glioblastoma cells were stimulated with NMDA to activate NMDAR glutamate receptors. The role of NMDAR activation on invasion and migration and its crosstalk with AMPAR were evaluated. Invasion and migration of glioblastoma cells were investigated by in vitro trans-well Matrigel invasion and trans-well migration assays, respectively. Expression of NMDARs and AMPARs at transcript level was evaluated by quantitative real-time polymerase chain reaction. Results We determined that NMDA stimulation leads to enhanced invasion in LN18, U251MG, and patient-derived glioblastoma cells, whereas inhibition of NMDAR using MK-801, a non-competitive antagonist of the NMDAR, significantly decreased the invasive capacity. Concordant with these findings, migration was significantly augmented by NMDAR in both cell lines. Furthermore, NMDA stimulation upregulated the expression of GluN2 and GluA1 subunits at the transcript level. Conclusions This study demonstrated the previously unexplored role of NMDAR in invasion of glioblastoma cells. Furthermore, the expression of the GluN2 subunit of NMDAR and the differential overexpression of the GluA1 subunit of AMPAR in both cell lines provide a plausible rationale of crosstalk between these calcium-permeable subunits in the glutamate-rich microenvironment of glioblastoma.

Preliminary Cytotoxic Activity of Sutherlandia frutescens and Carpobrotus edulis on Malignant glioblastoma Cells

2019 ◽  
Vol 3 (5) ◽  
pp. 175-179 ◽  
Author(s):  
Sylvester Omoruyi ◽  
◽  
Adaze Enogieru ◽  
Okobi Ekpo ◽  
◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Glioblastoma Cells ◽  
Carpobrotus Edulis ◽  
Sutherlandia Frutescens

FOXC2 Often Overexpressed in Glioblastoma Enhances Proliferation and Invasion in Glioblastoma Cells

2014 ◽  
Vol 21 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Weihua Li ◽  
Xin Fu ◽  
Rongyao Liu ◽  
Chunming Wu ◽  
Jingyang Bai ◽  
...  
Keyword(s):  
Glioblastoma Cells ◽  
Proliferation And Invasion

miR-221/222 Confers Radioresistance in Glioblastoma Cells Through Activating Akt Independent of PTEN Status

2014 ◽  
Vol 14 (1) ◽  
pp. 185-195 ◽  
Author(s):  
W. Li ◽  
F. Guo ◽  
P. Wang ◽  
S. Hong ◽  
C. Zhang
Keyword(s):  
Glioblastoma Cells

Effect of O6-Substituted Guanine Analogs on O6-methylguanine DNA-methyltransferase Expression and Glioblastoma Cells Viability

2016 ◽  
Vol 13 (1) ◽  
pp. 28-39 ◽  
Author(s):  
Patrick-Denis St-Coeur ◽  
Marc Cormier ◽  
Veronique LeBlanc ◽  
Pier Morin ◽  
Mohamed Touaibia
Keyword(s):  
Dna Methyltransferase ◽  
Glioblastoma Cells ◽  
Methylguanine Dna Methyltransferase
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