Nano-hydroxyapatite/fibrin glue/recombinant human osteogenic protein-1 artificial bone for repair of bone defect in an animal model

2012 ◽  
Vol 7 (5) ◽  
pp. 467 ◽  
Author(s):  
Weimin Zhu ◽  
Wei Lu ◽  
Xiaojun Zhang ◽  
Zhimin Cai ◽  
Haifeng Liu ◽  
...  
Keyword(s):  
Animal Model ◽  
Fibrin Glue ◽  
Bone Defect ◽  
Artificial Bone ◽  
Osteogenic Protein

Nano-Hydroxyapatite Scaffold Based on Recombinant Human Bone Morphogenetic Protein 2 and Its Application in Bone Defect Repair

2021 ◽  
Vol 17 (7) ◽  
pp. 1330-1338
Author(s):  
Shibai Zhu ◽  
Xiaotian Zhang ◽  
Xi Chen ◽  
Yiou Wang ◽  
Shanni Li ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Artificial Bone ◽  
Bone Defect Repair ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Defect Repair ◽  
Hydroxyapatite Scaffold ◽  
Recombinant Human Bone

The best way in which to prepare scaffolds with good biological properties is an urgent problem in the field of tissue engineering. In this paper we discuss the preparation of nano-hydroxyapatite scaffold of recombinant human bone morphogenetic protein-2 (rhBMP-2) and its application in bone defect repair. rhBMP-2 reagent was dissolved in 1 mol/L sodium dihydrogen phosphate solution, and the rhBMP-2 solution was added to the nano-hydroxyapatite artificial bone with a 100 μL glass micro dropper at the rate of 10 drops/min to obtain Nano-HA/rhBMP-2 composite artificial bone. In in vivo experiments, rabbits were fixed on an operating table, a 2 cm longitudinal incision was made in the middle part of the radial forearm, and the radius was cut with a wire saw and periosteum, 2.5 cm away from the distal radius. After washing the wound with normal saline, Adv-hBMP-2/MC3T3-E1 nano-HA composite artificial bone, MC3T3-E1 nan-HA composite artificial bone, or Nano-HA artificial bone were implanted in different groups. The artificial bone scaffold prepared in this study has a stronger ability to repair bone defects than the alternatives, and is a promising prospect for the clinical treatment of bone defects.

scholarly journals Physical and Histological Comparison of Hydroxyapatite, Carbonate Apatite, and β-Tricalcium Phosphate Bone Substitutes

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1993 ◽  
Author(s):  
Kunio Ishikawa ◽  
Youji Miyamoto ◽  
Akira Tsuchiya ◽  
Koichiro Hayashi ◽  
Kanji Tsuru ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Tricalcium Phosphate ◽  
Bone Substitutes ◽  
Physiological Solution ◽  
Tissue Response ◽  
Histological Evaluation ◽  
Carbonate Apatite ◽  
Artificial Bone ◽  
New Bone Formation

Three commercially available artificial bone substitutes with different compositions, hydroxyapatite (HAp; Neobone®), carbonate apatite (CO3Ap; Cytrans®), and β-tricalcium phosphate (β-TCP; Cerasorb®), were compared with respect to their physical properties and tissue response to bone, using hybrid dogs. Both Neobone® (HAp) and Cerasorb® (β-TCP) were porous, whereas Cytrans® (CO3Ap) was dense. Crystallite size and specific surface area (SSA) of Neobone® (HAp), Cytrans® (CO3Ap), and Cerasorb® (β-TCP) were 75.4 ± 0.9 nm, 30.8 ± 0.8 nm, and 78.5 ± 7.5 nm, and 0.06 m2/g, 18.2 m2/g, and 1.0 m2/g, respectively. These values are consistent with the fact that both Neobone® (HAp) and Cerasorb® (β-TCP) are sintered ceramics, whereas Cytrans® (CO3Ap) is fabricated in aqueous solution. Dissolution in pH 5.3 solution mimicking Howship’s lacunae was fastest in CO3Ap (Cytrans®), whereas dissolution in pH 7.3 physiological solution was fastest in β-TCP (Cerasorb®). These results indicated that CO3Ap is stable under physiological conditions and is resorbed at Howship’s lacunae. Histological evaluation using hybrid dog mandible bone defect model revealed that new bone was formed from existing bone to the center of the bone defect when reconstructed with CO3Ap (Cytrans®) at week 4. The amount of bone increased at week 12, and resorption of the CO3Ap (Cytrans®) was confirmed. β-TCP (Cerasorb®) showed limited bone formation at week 4. However, a larger amount of bone was observed at week 12. Among these three bone substitutes, CO3Ap (Cytrans®) demonstrated the highest level of new bone formation. These results indicate the possibility that bone substitutes with compositions similar to that of bone may have properties similar to those of bone.

Effect of Low Level Laser Therapy on Bone Defect of an Animal Model

2011 ◽  
Vol 38 (11) ◽  
pp. 1104004
Author(s):  
李秋实 Li Qiushi ◽  
周延民 Zhou Yanmin ◽  
陈英新 Chen Yingxin ◽  
柳淑杰 Liu Shujie ◽  
王战鑫 Wang Zhanxin ◽  
...  
Keyword(s):  
Animal Model ◽  
Laser Therapy ◽  
Bone Defect ◽  
Low Level Laser Therapy ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

A collagen membrane containing osteogenic protein-1 facilitates bone regeneration in a rat mandibular bone defect

2017 ◽  
Vol 84 ◽  
pp. 19-28 ◽  
Author(s):  
Manami Ozaki ◽  
Tadahiro Takayama ◽  
Takanobu Yamamoto ◽  
Yasumasa Ozawa ◽  
Mayu Nagao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Collagen Membrane ◽  
Mandibular Bone ◽  
Osteogenic Protein

Transplantation of Dental Pulp Stem Cells in Experimental Bone Defect

2017 ◽  
Vol 34 ◽  
pp. 94-100 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Fatma S. Dewi ◽  
Ahmad Aulia Yusuf ◽  
Rahmi Ulfiana
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defect ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Bone Tissue Regeneration ◽  
Histological Evaluation ◽  
Control Rabbit

This is preliminary study in order to investigate the effect of dental pulp stem cells (DPSCs) on bone regeneration in an animal model. New Zealand rabbits were used as animal model. The critical defect was created in femoral bone and transplantation of DPSCs applied into bone defect. A colorimetric assay was used to detect ALP level in rabbit’s serum. Bone tissue regeneration was evaluated by histological analysis. In the 2nd week, the treated rabbit show increasing in the activity of ALP (157,925 μU) compared to control rabbit (155,361 μU). This increasing trend continues significantly in DPSCs rabbit (169.750 μU) compared to control rabbit (160.406) after 4 weeks. Histological evaluation revealed that the amount of bone lamellae and osteocytes were filled the defect area of DPSCs treated rabbit. Conclusions: Transplantation of DPSCs accelerating bone regeneration by raising ALP level and forming new bone tissue.

An Animal Model to Study Bisphosphonates Associated Osteonecrosis of the Jaw

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3659-3659
Author(s):  
Gabriele Pozzato ◽  
Silvia Chiandussi ◽  
Serena Zacchigna ◽  
Franca Dore ◽  
Silvia Moimas ◽  
...  
Keyword(s):  
Animal Model ◽  
Zoledronic Acid ◽  
Bone Defect ◽  
Mature Female ◽  
Osteonecrosis Of The Jaw ◽  
Oral Bacteria ◽  
Control Group ◽  
Imaging Features ◽  
Female Wistar Rats ◽  
Bone Exposure

Abstract The pathogenesis of osteonecrosis of the jaws (ONJ) in patients treated with intravenous bisphosphonates is still not completely understood, and likely involves both reduction in blood supply and the activity of oral bacteria. We present an animal model of ONJ, which will significant contribute to the understanding of the aetiopathogenesis of this condition. Five skeletally-mature female Wistar rats (weight 450 g) were given intravenous zoledronic acid 0.04 mg in saline solution (0.2 mg/ml) once a week for 5 weeks. After 2 weeks, the animals underwent the extraction of the upper right first molar, followed by the creation of a 4 mm-diameter bone defect on the same site under general anaesthesia. After 7 weeks from the extraction, the animals were clinically examined and a static bone-scintigraphy using 99mTc-MDP methylene diphosphonate was performed with a gamma camera equipped with pinhole collimator (Siemens-Ecam). After an additional week, the rats were sacrificed and a Computed Tomography (CT) was carried out. Samples obtained from the bone defect were decalcified and prepared for histological assessment. Five rats, not treated with zoledronic acid and exposed to the same surgical treatment, were used as controls. Histological and imaging features were assessed blindly. At 7 weeks after the extraction, all the rats treated with zoledronic acid showed impair healing, expansion of the defect and bone exposure. These features were confirmed by the scintigraphy, which showed abnormal localized activity in comparison with the surrounding tissues. On clinical examination, the rats of the control group demonstrated epithelialization of the bone defect and a normal uptake of the contrast medium during the scan. The CT scan disclosed irregularity of the cortical margin and destruction of the cortical bone, which were not evident in the control group. The histology showed sheets of necrotic bone, with loss of osteocytes from their lacunae and peripheral resorption. No inflammatory infiltrate was observed. The control group instead demonstrated normal bone healing. All animals were treated according with the guiding principles for experimental procedures found in the Declaration of Helsinki of the World Medical Association. Based on this study, the rat treated with zoledronic acid can be considered a novel, reliable and reproducible model to better understand the pathophysiology of ONJ and to develop a therapeutic approach.

scholarly journals Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model

2016 ◽  
Vol 25 (7) ◽  
pp. 1405-1414 ◽  
Author(s):  
Lukas Skoloudik ◽  
Viktor Chrobok ◽  
David Kalfert ◽  
Zuzana Koci ◽  
Eva Sykova ◽  
...  
Keyword(s):  
Animal Model ◽  
Temporal Bone ◽  
Bone Defect ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells
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