scholarly journals Biosynthesis of selenium nanoparticles by Aspergillus flavus and Candida albicans for antifungal applications

2021 ◽  
Author(s):  
Mahdi Hosseini Bafghi ◽  
Majid Darroudi ◽  
Mohsen Zargar ◽  
Hossein Zarrinfar ◽  
Razieh Nazari
Keyword(s):  
Candida Albicans ◽  
Aspergillus Flavus ◽  
Selenium Nanoparticles

Biosynthesis of selenium nanoparticles by Aspergillus flavus and Candida albicans and comparison of their effects with antifungal drugs

2021 ◽  
Author(s):  
Mahdi Hosseini Bafghi ◽  
Razieh Nazari ◽  
Majid Darroudi ◽  
Mohsen Zargar ◽  
Hossein Zarrinfar
Keyword(s):  
Candida Albicans ◽  
Aspergillus Flavus ◽  
Fungal Pathogens ◽  
Chemical Properties ◽  
Cost Effective ◽  
Antifungal Drugs ◽  
Selenium Nanoparticles ◽  
Fungal Strains ◽  
Vis Spectroscopy ◽  
Physical And Chemical

Abstract Biosynthesis of nanoparticles can stand as a replacement for the available chemical and physical methods by offering new procedures as green syntheses that have proved to be simple, biocompatible, safe, and cost-effective. Considering how nanoparticles with a size of 1 to 100 nanometers contain unique physical and chemical properties, recent reports are indicative of observing the antifungal qualities of selenium nanoparticles (Se-NPs). Recently, the observance of antifungal resistance towards different species of these fungi is often reported. Therefore, due to the antifungal effects of biological nanoparticles, this study aimed to investigate the exertion of these nanoparticles and evaluate their effects on the growth of fungal pathogens. Se-NPs were biosynthesized by the application of wet reduction method, which included specific concentrations of Aspergillus flavus and Candida albicans. The presence of nanoparticles was confirmed by methods such as UV-Vis spectroscopy, FT-IR analysis, and FESEM electron microscope that involved FESEM and EDAX diagram. The fungal strains were cultured in sabouraud dextrose agar medium to perform the sensitivity test based on the minimum inhibitory concentration (MIC) method in duplicate. The utilization of Se-NPs at concentrations of 1 µg/ ml and below resulted in zero growth of fungal agents. However, their growth was inhibited by antifungal drugs at concentrations of 2 µg/ ml and higher. Based on the obtained results, biological nanoparticles produced by fungal agents at different concentrations exhibited favorable inhibitory effects on the growth of fungal strains.

scholarly journals Effect of biogenic selenium nanoparticles on ERG11 and CDR1 gene expression in both fluconazole-resistant and -susceptible Candida albicans isolates

2017 ◽  
Vol 3 (3) ◽  
pp. 16-20 ◽  
Author(s):  
Nasrin Parsamehr ◽  
Sasan Rezaie ◽  
Sadegh Khodavaisy ◽  
Samira Salari ◽  
Sanaz Hadizadeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Selenium Nanoparticles ◽  
Fluconazole Resistant ◽  
Biogenic Selenium Nanoparticles

scholarly journals BIOAKTIVITAS JAMUR Aspergillus flavus YANG BERSIMBION DENGAN ASCIDIAN Eudistoma sp.

2020 ◽  
Vol 8 (2) ◽  
pp. 11
Author(s):  
Alfa Mongi ◽  
Deiske A Sumilat ◽  
Fitje Losung ◽  
Remmy E P Mangindaan ◽  
Rosita A Lintang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Aspergillus Flavus ◽  
Marine Invertebrate ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts

Ascidian is a marine invertebrate that produce bioactive compounds such as antibacterial and antifungal. The purpose of this study was to obtain symbiotic fungi isolates, ethyl acetate extracts from ascidian symbiotic isolates, and to determine the antibacterial and antifungal activities of ethyl acetate extracts of symbiotic isolates using the diffusion method (disc diffusion Kirby and Bauer) against Escherichia coli, Staphylococcus aureus bacteria and against the Candida albicans fungus. The results obtained 1 ascidian species which was cultured to obtain pure isolates, the pure isolates were tested molecularly and the results showed that the isolate was Aspergillus flavus, then the isolates were grown on rice medium and extracted. The results of the fungal extract showed the presence of antibacterial and antifungal activity against Escherichia coli by 10 mm, Staphylococcus aureus by 19 mm and against the Candida albicans by 13.5 mm. Keywords : Ascidian, Eudistoma sp., Aspergillus flavus, Antibacterial, Antifungal, Symbiotic.                                                  AbstrakAscidian merupakan avertebrata laut yang dapat menghasilkan senyawa bioaktif yang seperti antibakteri dan antijamur. Tujuan dari penelitian ini yaitu mendapatkan isolat jamur simbion dari ascidian, mendapatkan ekstrak etil asetat dari isolat jamur simbion ascidian, dan menguji aktivitas antibakteri dan antijamur ekstrak etil asetat isolat jamur simbion dengan metode difusi agar (disc diffusion Kirby and Bauer) terhadap bakteri Escherichia coli, Staphylococcus aureus dan terhadap jamur Candida albicsans. Hasil penelitian didapatkan 1 jenis ascidian yang kemudian dikultur untuk mendapatkan isolat murni, isolat murni diuji secara molekuler dan didapati hasil bahwa isolat tersebut merupakan jamur Aspergillus flavus. Isolat tersebut kemudian ditumbuhkan pada media nasi dan diekstrak. Hasil ekstrak jamur tersebut menunjukkan adanya aktivitas antibakteri dan antijamur terhadap bakteri Escherichia coli sebesar 10 mm, Staphylococcus aureus sebesar 19 mm dan terhadap jamur Candida albicsans 13,5 mm. Kata kunci : Ascidian, Eudistoma sp., Aspergillus flavus, Antibakteri, Antijamur, Simbion.

scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

2018 ◽  
Vol 11 (1) ◽  
pp. 408
Author(s):  
Kassim Dekhil
Keyword(s):  
Candida Albicans ◽  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Influencing Factors ◽  
Aspergillus Flavus ◽  
Species Distribution ◽  
Alternaria Alternata ◽  
Otitis Externa ◽  
Fungal Species ◽  
The Public

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

scholarly journals BIODEGRADATION POTENTIAL OF RHIZOSPHERIC MICROORGANISMS OF RHIZOPHORA RACEMOSA IN CRUDE OIL CONTAMINATED MANGROVE SWAMP IN THE NIGER DELTA

2021 ◽  
Vol 2 (2) ◽  
pp. 91-102
Author(s):  
O. R. Aina ◽  
E. I. Atuanya ◽  
C. E. Oshoma ◽  
A. E. Omotayo ◽  
O. N. Olaleye
Keyword(s):  
Candida Albicans ◽  
Crude Oil ◽  
Aspergillus Flavus ◽  
Niger Delta ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Mineral Salts ◽  
Mangrove Swamp ◽  
Rhizospheric Microorganisms ◽  
Acinetobacter Sp

Rhizophora racemosa (red mangrove tree) belongs to the family Rhizophoraceae; it is an important constituent of the mangrove swamp in Niger Delta, an oil producing region in Nigeria. The remediation of soils containing organic pollutants is possible with the use of microbial communities when the ecology is understood for potentials maximization. This study investigated the biodegradation potential of rhizospheric microorganisms of Rhizophora racemosa in crude oil- contaminated mangrove swamp in the Niger Delta.The total microbial count was determined by the serial dilution method. The hydrocarbon-utilizing bacteria and fungi were enumerated using Mineral Salts Agar containing crude oil as the sole carbon source. The biodegradation potential of these rhizomicrobes was determined using screen test, shake flask degradation tests, Total Organic Gas (TOG) and Total Petroleum Hydrocarbon (TPH) InfraCal Analyzer (HATR-T2 and CH). The turbidity, total organic gas (TOG-N) and total petroleum hydrocarbon were measured weekly for twenty-eight days. Hydrocarbon-degrading microbes isolated from the rhizosphere were identified as Marinococcus sp., Azotobacter sp., Acinetobacter sp. Aspergillus niger, Aspergillus flavus and Candida albicans. The highest rate of TPH reduction was recorded in Acinetobacter sp. (from 150 mg/L on day 1 to ˂0.0031 mg/L on day 14). This was followed by Candida albicans (148mg/L on day 1 to 2.68mg/L on day 28) and Aspergillus flavus (150mg/L on day 1 to 4.21mg/L on day 28) In conclusion, it can be inferred that the some rhizospheric microbes of Rhizophora racemosa can efficienctly degrade hydrocarbon up to 100% rate over a period of 28 days.

scholarly journals Antifungal Activity of Selenium Nanoparticles Extracted from Capparis decidua Fruit against Candida albicans

2020 ◽  
Vol 9 (34) ◽  
pp. 2452-2455
Author(s):  
Shifa Jawahar Ali ◽  
Preetha S ◽  
Jeevitha M ◽  
Lavanya Prathap ◽  
Rajeshkumar S.
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Selenium Nanoparticles ◽  
Capparis Decidua

scholarly journals Purified Quercetin From in vitro Cell Suspension Cultures of Caesalpinia pulcherrima Sw. and Its Antimicrobial Potentials

2017 ◽  
Vol 9 (1) ◽  
pp. 14
Author(s):  
GM Greeshma ◽  
JM Aswathy ◽  
Murukan Greeshma ◽  
Lawarence Bosco ◽  
Murugan K
Keyword(s):  
Candida Albicans ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Enterococcus Faecalis ◽  
Aspergillus Flavus ◽  
Cell Suspension Culture ◽  
Trichophyton Rubrum ◽  
Gram Negative Bacteria ◽  
Unknown Compound ◽  
Gram Negative

Tribal people use the flower extract of <em>Caesalpinia pulcherrima<strong> </strong></em>to cure liver, stomach and skin disorders in Indian traditional medicine. This study aimed to evaluate the protective roles of purified quercetin extracted from suspension culture of <em>C. pulcherrima</em> against selected bacterial and fungal pathogens. A simple protocol was developed for callus production using leaf explants<em>.</em> 2, 4-D (2.5 mg/l), BAP (2.5 mg/l) + kin (1 mg/ml) was effective for optimal callus induction. Subsequently, cell suspension culture was established.  Role of effect of elicitors in cell suspension culture was carried. Sucrose, ABA and salicylic acid (SA) at different concentrations influenced cell biomass and quercetin accumulation. Cells cultured in the medium fortified with 45 g/L sucrose without ABA/SA showed the highest quercetin content (16.5 mg/g). Flavonoids was purified, fractionated by HPLC-DAD followed by NMR revealed the presence of quercetin, isoquercetin, quercetrin, rutin, quercetin 3-O-β-D-xyloside, quercetin 3-Oarabinopyranoside, quercetin 3-O- α-arabinopyranosyl (1→2) β-galactopyranoside,  isorhamnetin 3-O-rutinoside and an unknown compound. Subsequently, anthocyanin was evaluated for antimicrobial activity against selected Gram-positive bacteria (<em>S. aureus, Bacillus subtilis</em>, and <em>Enterococcus faecalis</em><em>)</em>, Gram-negative bacteria (<em>E. coli, </em>and <em>Pseudomonas aeruginosa</em>) and fungi such as <em>Aspergillus flavus, Candida albicans </em>and <em>Trichophyton rubrum</em>. Quercetin was found to be active against four bacteria and the fungi- <em>Candida albicans</em>. The highest inhibitory effects were found on <em>S. aureus</em> and <em>Enterococcus faecalis</em>. Gram negative bacteria showed more resistance i.e., with insignificant MIC and MBC values. Among the fungi, <em>Aspergillus flavus</em> and <em>Trichophyton rubrum</em> displayed remarkable MIC and MKC values. These results suggest that quercetin may be used as a natural antimicrobial agent. Future works are designed to trace the molecular mechanism of antimicrobial potentiality of quercetin against these tested pathogens

Anti-Fungal Activity of Cressa cretica, Leptadenia pyrotechnica and Pulicaria crispa, indigenous plants of Cholistan desert, Pakistan

2020 ◽  
Vol 18 ◽  
Author(s):  
Faheem Hadi ◽  
Tahir Maqbool ◽  
Sameera Khurshid ◽  
Aisha Nawaz ◽  
Saira Aftab ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Fungal Infections ◽  
Fungal Species ◽  
Fungal Activity ◽  
Aspergillus Ustus ◽  
Mother Tincture ◽  
Anti Fungal

Background: Fungal infections have always remain a problem and they are getting worse with passage of each year due to their resistance against available antibiotics. Natural ways of treatment with homoeopathic medicines made of mother tinctures of plants have no reported side effects and have been proved effective against many bacterial and fungal infections. Materials & Methods: Preparation of mother tinctures of plants Cressa cretica, Leptadenia pyrotechnica and Pulicaria crispa was done and used to evaluate the anti-fungal potential of these plants against potentially pathogenic fungal species like Aspergillus niger, Aspergillus flavus, Aspergillus ustus and Candida albicans by agar disc diffusion method. Each tincture was evaluated at 0.25 ml, 0.5 ml and 1 ml volume per disc and zone of inhibition was measured in millimetres and compared with commercial drug Fluconazole (2 mg/ml) which was used as standard. Results: The results showed that satisfactory anti-fungal activity of these plants in comparison to standard drug Fluconazole. The ratio of antifungal activity of Cressa cretica measured as the zone of growth inhibition of these cultures against Aspergillus niger, Aspergillus flavus, Aspergillus ustus and Candida albicans was 24 mm / 27 mm, 30mm / 28 mm, 23 mm/ 30 mm and 32 mm/ 30 mm respectively. In the case of Leptadenia pyrotechnica, the mother tincture’s antifungal activity was 32 mm / 27 mm, 30 mm / 28 mm, 17 mm/ 30 mm and 24 mm/ 30 mm. In case of our third plant Pulicaria crispa, antifungal activity came out to be 23 mm/ 27 mm, 26 mm/ 28 mm, 26 mm / 30 mm and 24 mm/ 30 mm. Conclusion: Our study proved that mother tincture of these plants can be a potential new therapy to treat fungal infections and has the potential to rule out fungal problems. Further research using mother tincture of these plants against other fungal species has the potential to prove them a safer and widespread anti-fungal homoeopathic medicine.

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