Membrane channels as a tool to control nanoreactors

2006 ◽  
Vol 153 (4) ◽  
pp. 107 ◽  
Author(s):  
M. Lindemann ◽  
M. Winterhalter
Keyword(s):  
Membrane Channels

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

Millisecond-Long Simulations of Antibiotics Transport through Outer Membrane Channels

2020 ◽  
Author(s):  
Vinaya Kumar Golla ◽  
Jigneshkumar Dahyabhai Prajapati ◽  
Ulrich Kleinekathöfer
Keyword(s):  
Outer Membrane ◽  
Membrane Channels

scholarly journals Sparse Labeling PELDOR Spectroscopy on Multimeric Mechanosensitive Membrane Channels

2017 ◽  
Vol 113 (9) ◽  
pp. 1968-1978 ◽  
Author(s):  
Katrin Ackermann ◽  
Christos Pliotas ◽  
Silvia Valera ◽  
James H. Naismith ◽  
Bela E. Bode
Keyword(s):  
Membrane Channels ◽  
Sparse Labeling

Contribution of non-selective membrane channels and receptors in epilepsy

2021 ◽  
pp. 107980
Author(s):  
Claudia García-Rodríguez ◽  
Iván D. Bravo-Tobar ◽  
Yorley Duarte ◽  
Luis C. Barrio ◽  
Juan C. Sáez
Keyword(s):  
Membrane Channels ◽  
Selective Membrane

Amphotericin B Covalent Dimers Forming Sterol-Dependent Ion-Permeable Membrane Channels

2002 ◽  
Vol 124 (16) ◽  
pp. 4180-4181 ◽  
Author(s):  
Nobuaki Matsumori ◽  
Nahoko Yamaji ◽  
Shigeru Matsuoka ◽  
Tohru Oishi ◽  
Michio Murata
Keyword(s):  
Amphotericin B ◽  
Membrane Channels ◽  
Permeable Membrane
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