Pattern modification of a neuronal network for individual-cell-based electrophysiological measurement using photothermal etching of an agarose architecture with a multielectrode array

2004 ◽  
Vol 151 (3) ◽  
pp. 116 ◽  
Author(s):  
I. Suzuki ◽  
Y. Sugio ◽  
H. Moriguchi ◽  
A. Hattori ◽  
K. Yasuda ◽  
...  
Keyword(s):  
Neuronal Network ◽  
Individual Cell ◽  
Multielectrode Array ◽  
Electrophysiological Measurement

A microchannel device tailored to laser axotomy and long-term microelectrode array electrophysiology of functional regeneration

Lab on a Chip ◽  
2015 ◽  
Vol 15 (24) ◽  
pp. 4578-4590 ◽  
Author(s):  
Rouhollah Habibey ◽  
Asiyeh Golabchi ◽  
Shahrzad Latifi ◽  
Francesco Difato ◽  
Axel Blau
Keyword(s):  
Neuronal Network ◽  
Microelectrode Array ◽  
Laser Microdissection ◽  
Axonal Injury ◽  
Multielectrode Array ◽  
Pdms Microchannel ◽  
Functional Regeneration ◽  
Microchannel Device ◽  
Network Morphology

We present a PDMS microchannel device compatible with multielectrode array electrophysiology and laser microdissection for selected axonal injury and long-term access to compartmentalized neuronal network morphology and activity.

scholarly journals Flexible Microfabrication on a Chip during Cultivation for a Neuronal Network Direction Control Using Stepwise Photo-Thermal Etching of an Agarose Architecture

2021 ◽  
Vol 4 (1) ◽  
pp. 4
Author(s):  
Yuhei Tanaka ◽  
Haruki Watanabe ◽  
Kenji Shimoda ◽  
Kenji Yasuda
Keyword(s):  
Neuronal Network ◽  
Cultured Cells ◽  
Individual Cell ◽  
Synaptic Connections ◽  
Flexible Control ◽  
Direction Control ◽  
Potential Damage ◽  
On Chip ◽  
Microfabrication Technology ◽  
Network Patterns

Control over spatial distributions and patterns of individual neurons and their neurites provides an essential tool for studying the meaning of neuronal network patterns. Moreover, the complete direction control of synaptic connections between cells in each neuronal network is also essential to investigate detailed information on the relationship between the forward and feedback signaling among the cells. Here, we have developed a method for topographical control of the direction of synaptic connections within a living neuronal network using a new type of individual-cell-based on-chip cell-cultivation system with an agarose microfabrication technology. The advantages of this system include the ability to control positions and number of cultured cells, as well as flexible control of the direction of elongation of axons and dendrites with stepwise melting of a thin agarose layer coated on the cultivation chip with a focused infrared laser beam even during cultivation without any destructive damage on cells. Using this system, we succeeded in forming a fully direction-controlled single-cell-based neuronal network from individual rat hippocampal cells. In this meeting, we discuss the potential damage of heat to cells during stepwise melting of agarose and demonstrate the ability of our on-chip agarose microfabrication method for individual cell-based neural networks.

Ultrastructure of myoepithelial cell in parotid gland

1988 ◽  
Vol 46 ◽  
pp. 342-343
Author(s):  
C. N. Sun
Keyword(s):  
Parotid Gland ◽  
Osmium Tetroxide ◽  
Individual Cell ◽  
Branching Processes ◽  
Muscle Cells ◽  
Myoepithelial Cells ◽  
Uranyl Acetate ◽  
Thin Sections ◽  
Gland Carcinoma ◽  
Parotid Gland Carcinoma

Myoepithelial cells have been observed in the prostate, harderian, apocrine, exocrine sweat and mammary glands. Such cells and their numerous branching processes form basket-like structures around the glandular acini. Their shapes are quite different from structures seen either in spindleshaped smooth muscle cells or skeletal muscle cells. These myoepithelial cells lie on the epithelial side of the basement membrane in the glands. This presentation describes the ultrastructure of such myoepithelial cells which have been found also in the parotid gland carcinoma from a 45-year old patient.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4 percent glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate. Ultrastructurally, the pattern of each individual cell showed wide variations.

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

Sleep is a neuronal-network matter

Nature ◽  
2017 ◽  
Vol 546 (7660) ◽  
pp. 579-579
Keyword(s):  
Neuronal Network
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