Passive and active MM-wave people screening systems

2006 ◽  
Author(s):  
B. Lyons ◽  
E. Entchev ◽  
R. Doyle ◽  
P. Curtin ◽  
J. McNaboe ◽  
...  
Keyword(s):  
Screening Systems

Development of the X-ray Security Screening Systems at ADANI

2019 ◽  
pp. 249-259
Author(s):  
D. A. Bairashewski ◽  
G. Yu. Drobychev ◽  
V. A. Karas ◽  
V. V. Komarov ◽  
M. V. Protsko
Keyword(s):  
Security Screening ◽  
X Ray ◽  
Screening Systems

Sample/analyte screening systems and chromatography

2001 ◽  
Vol 53 (S1) ◽  
pp. S149-S153 ◽  
Author(s):  
M. Valcárcel ◽  
M. Gallego ◽  
S. Cárdenas
Keyword(s):  
Screening Systems

scholarly journals An Effective Counterselection System for Listeria monocytogenes and Its Use To Characterize the Monocin Genomic Region of Strain 10403S

2016 ◽  
Vol 83 (6) ◽  
Author(s):  
Tal Argov ◽  
Lev Rabinovich ◽  
Nadejda Sigal ◽  
Anat A. Herskovits
Keyword(s):  
Listeria Monocytogenes ◽  
Point Mutations ◽  
Model Organism ◽  
Trna Synthetase ◽  
Genomic Region ◽  
Selection Marker ◽  
Content Type ◽  
Allelic Exchange ◽  
Suicide Vector ◽  
Screening Systems

ABSTRACT Construction of Listeria monocytogenes mutants by allelic exchange has been laborious and time-consuming due to lack of proficient selection markers for the final recombination event, that is, a marker conveying substance sensitivity to the bacteria bearing it, enabling the exclusion of merodiploids and selection for plasmid loss. In order to address this issue, we engineered a counterselection marker based on a mutated phenylalanyl-tRNA synthetase gene (pheS*). This mutation renders the phenylalanine-binding site of the enzyme more promiscuous and allows the binding of the toxic p-chloro-phenylalanine analog (p-Cl-phe) as a substrate. When pheS* is introduced into L. monocytogenes and highly expressed under control of a constitutively active promoter, the bacteria become sensitive to p-Cl-phe supplemented in the medium. This enabled us to utilize pheS* as a negative selection marker and generate a novel, efficient suicide vector for allelic exchange in L. monocytogenes. We used this vector to investigate the monocin genomic region in L. monocytogenes strain 10403S by constructing deletion mutants of the region. We have found this region to be active and to cause bacterial lysis upon mitomycin C treatment. The future applications of such an effective counterselection system, which does not require any background genomic alterations, are vast, as it can be modularly used in various selection systems (e.g., genetic screens). We expect this counterselection marker to be a valuable genetic tool in research on L. monocytogenes. IMPORTANCE L. monocytogenes is an opportunistic intracellular pathogen and a widely studied model organism. An efficient counterselection marker is a long-standing need in Listeria research for improving the ability to design and perform various genetic manipulations and screening systems for different purposes. We report the construction and utilization of an efficient suicide vector for allelic exchange which can be conjugated, leaves no marker in the bacterial chromosome, and does not require the use of sometimes leaky inducible promoters. This highly efficient genome editing tool for L. monocytogenes will allow for rapid sequential mutagenesis, introduction of point mutations, and design of screening systems. We anticipate that it will be extensively used by the research community and yield novel insights into the diverse fields studied using this model organism.

scholarly journals CAN INSILICO SCREENING SYSTEMS PROVIDE US WITH THE TOOLS TO PREDICT THE DMPK PROPERTIES OF DRUGS IN THE DISCOVERY PROCESS?

2001 ◽  
Vol 16 (supplement) ◽  
pp. 162-163 ◽  
Author(s):  
Toshiyuki KUME ◽  
Tsunehiro HARADA ◽  
Katsuyuki FUKUDA ◽  
Hideshi SHIMADZU
Keyword(s):  
Discovery Process ◽  
Screening Systems
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