Real-time ultrasonic process tomography for two-component flows

1996 ◽  
Vol 32 (17) ◽  
pp. 1571 ◽  
Author(s):  
H.I. Schlaberg ◽  
M. Yang ◽  
B.S. Hoyle
Keyword(s):  
Real Time ◽  
Process Tomography ◽  
Ultrasonic Process ◽  
Two Component

Wide-angle transducers for real-time ultrasonic process tomography imaging applications

Ultrasonics ◽  
1997 ◽  
Vol 35 (3) ◽  
pp. 213-221 ◽  
Author(s):  
H.I. Schlaberg ◽  
M. Yang ◽  
B.S. Hoyle ◽  
M.S. Beck ◽  
C. Lenn
Keyword(s):  
Real Time ◽  
Wide Angle ◽  
Tomography Imaging ◽  
Process Tomography ◽  
Ultrasonic Process

scholarly journals Signal Pathway in Salt-Activated Expression of the Salmonella Pathogenicity Island 1 Type III Secretion System in Salmonella enterica Serovar Typhimurium

2008 ◽  
Vol 190 (13) ◽  
pp. 4624-4631 ◽  
Author(s):  
Hideaki Mizusaki ◽  
Akiko Takaya ◽  
Tomoko Yamamoto ◽  
Shin-Ichi Aizawa
Keyword(s):  
Electron Microscopy ◽  
Real Time ◽  
Protein Secretion ◽  
Real Time Pcr ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Sds Page ◽  
Two Component ◽  
Serovar Typhimurium

ABSTRACT Salmonella enterica serovar Typhimurium secretes virulence factors for invasion called Sip proteins or Sips into its hosts through a type III secretion system (T3SS). In the absence of a host, S. enterica induces Sip secretion in response to sucrose or simple salts, such as NaCl. We analyzed induction of host-independent Sip secretion by monitoring protein secretion by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), assembly of needle complexes by electron microscopy, and transcription of virulence regulatory genes by quantitative reverse transcriptase PCR (real-time PCR). SDS-PAGE showed that addition of sucrose or simple salts, such as NaCl, to the growth medium induced Sip secretion without altering flagellar protein secretion, which requires a distinct T3SS. Electron microscopy confirmed that the amount of secreted Sips increased as the number of assembled needle complexes increased. Real-time PCR revealed that added sucrose or NaCl enhanced transcription of hilA, hilC, and hilD, which encode known regulators of Salmonella virulence. However, epistasis analysis implicated HilD and HilA, but not HilC, in the direct pathway from the salt stimulus to the Sip secretion response. Further analyses showed that the BarA/SirA two-component signal transduction pathway, but not the two-component sensor kinase EnvZ, directly activated hilD and hilA transcription and thus Sip secretion in response to either sucrose or NaCl. Finally, real-time PCR showed that salt does not influence transcription of the BarA/SirA-dependent csrB and csrC genes. A model is proposed for the major pathway in which sucrose or salt signals to enhance virulence gene expression.

scholarly journals Regulation of virulence and β-lactamase gene expression in Staphylococcus aureus isolates: cooperation of two-component systems in bloodstream superbugs

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sanaz Dehbashi ◽  
Hamed Tahmasebi ◽  
Behrouz Zeyni ◽  
Mohammad Reza Arabestani
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Gene Expressions ◽  
Resistance Development ◽  
Component Systems ◽  
Two Component ◽  
Two Component Systems ◽  
Lactamase Gene

Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA)-bloodstream infections (BSI) are predominantly seen in the hospital or healthcare-associated host. Nevertheless, the interactions of virulence factor (VFs) regulators and β-lactam resistance in MRSA-BSI are unclear. This study aims to characterize the molecular relationship of two-component systems of VFs and the expression of the β-lactamase gene in MRSA-BSI isolates. In this study, 639 samples were collected from BSI and identified by phenotypic methods. We performed extensive molecular characterization, including SCCmec type, agr type, VFs gene profiles determinations, and MLST on isolates. Also, a quantitative real-time PCR (q-RT PCR) assay was developed for identifying the gene expressions. Results Ninety-one (91) S. aureus and 61 MRSA (67.0%) strains were detected in BSI samples. The presence of VFs and SCCmec genes in MRSA isolates were as follows: tst (31.4%), etA (18.0%), etB (8.19%), lukS-PVL (31.4%), lukF-PV (18.0%), lukE-lukD (16.3%), edin (3.2%), hla (16.3%), hlb (18.0%), hld (14.7%), hlg (22.9%), SCCmecI (16.3%), SCCmecII (22.9%), SCCmecIII (36.0%), SCCmecIV (21.3%), and SCCmecV (16.3%). Quantitative real-time PCR showed overexpression of mecRI and mecI in the toxigenic isolates. Moreover, RNAIII and sarA genes were the highest expressions of MRSA strains. The multi-locus sequence typing data confirmed a high prevalence of CC5, CC8, and CC30. However, ST30, ST22, and ST5 were the most prevalent in the resistant and toxigenic strains. Conclusion We demonstrated that although regulation of β-lactamase gene expressions is a significant contributor to resistance development, two-component systems also influence antibiotic resistance development in MRSA-BSI isolates. This indicates that resistant strains might have pathogenic potential. We also confirmed that some MLST types are more successful colonizers with a potential for MRSA-BSI.

A Review on Ultrasonic Process Tomography System

2014 ◽  
Vol 70 (3) ◽  
Author(s):  
Sallehuddin Ibrahim ◽  
Mohd Amri Md Yunus ◽  
Mohd Taufiq Md Khairi ◽  
Mahdi Faramarzi
Keyword(s):  
Ultrasonic Tomography ◽  
Medical Field ◽  
Tomography System ◽  
Process Tomography ◽  
Ultrasonic Process ◽  
Process Vessel

The advantage of using a tomography system is that it provides visualization of component distributions inside a pipe or a process vessel. Tomography is widely used in the medical field. Due to its non-intrusive nature, tomography has caught the interest of various researchers in process applications. There is an extensive research using ultrasonic tomography for various applications. This paper is a review which expounds the principles of ultrasonic tomography systems, the hardware, and the software used in such systems.

scholarly journals Real time propagation of the exact two component time-dependent density functional theory

2016 ◽  
Vol 145 (10) ◽  
pp. 104107 ◽  
Author(s):  
Joshua J. Goings ◽  
Joseph M. Kasper ◽  
Franco Egidi ◽  
Shichao Sun ◽  
Xiaosong Li
Keyword(s):  
Density Functional Theory ◽  
Real Time ◽  
Density Functional ◽  
Time Dependent ◽  
Functional Theory ◽  
Two Component ◽  
Time Propagation ◽  
Component Time ◽  
Dependent Density

scholarly journals Real-time in situ dynamic sub-surface imaging of multi-component electrodeposited films using event mode neutron reflectivity

2018 ◽  
Vol 210 ◽  
pp. 429-449 ◽  
Author(s):  
A. Robert Hillman ◽  
Robert Barker ◽  
Robert M. Dalgliesh ◽  
Virginia C. Ferreira ◽  
Emma J. R. Palin ◽  
...  
Keyword(s):  
Real Time ◽  
Deep Eutectic Solvent ◽  
Neutron Reflectivity ◽  
Surface Imaging ◽  
Two Component ◽  
Metal Layers

We investigate the deposition, evolution and dissolution of single and two-component metal layers on Au substrates immersed in the deep eutectic solvent Ethaline.

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