Characterization of Human Atherosclerotic Plaques Using Multimodal Multiphoton Microscopy

2016 ◽  
Author(s):  
E. Baria ◽  
R. Cicchi ◽  
G. Nesi ◽  
D. Massi ◽  
F.S. Pavone
Keyword(s):  
Multiphoton Microscopy ◽  
Atherosclerotic Plaques

Review: Mechanical Characterization of Carotid Arteries and Atherosclerotic Plaques

2016 ◽  
Vol 63 (10) ◽  
pp. 1613-1623 ◽  
Author(s):  
Chris L. de Korte ◽  
Stein Fekkes ◽  
Aart J. Nederveen ◽  
Rashindra Manniesing ◽  
Hendrik Rik H. G. Hansen
Keyword(s):  
Carotid Arteries ◽  
Mechanical Characterization ◽  
Atherosclerotic Plaques

scholarly journals Characterization of Atherosclerotic Plaques by Laser Speckle Imaging

Circulation ◽  
2005 ◽  
Vol 112 (6) ◽  
pp. 885-892 ◽  
Author(s):  
Seemantini K. Nadkarni ◽  
Brett E. Bouma ◽  
Tina Helg ◽  
Raymond Chan ◽  
Elkan Halpern ◽  
...  
Keyword(s):  
Laser Speckle ◽  
Atherosclerotic Plaques ◽  
Laser Speckle Imaging ◽  
Speckle Imaging

Expression and Cellular Localization of CXCR4 and CXCL12 in Human Carotid Atherosclerotic Plaques

2018 ◽  
Vol 118 (01) ◽  
pp. 195-206 ◽  
Author(s):  
Sophie Merckelbach ◽  
Emiel van der Vorst ◽  
Michael Kallmayer ◽  
Christoph Rischpler ◽  
Rainer Burgkart ◽  
...  
Keyword(s):  
Protein Level ◽  
Cellular Localization ◽  
Mrna Level ◽  
Plaque Morphology ◽  
Atherosclerotic Plaques ◽  
Cxcl12 Expression ◽  
Carotid Plaques ◽  
Unstable Plaques ◽  
Human Carotid

Background and Aims The CXCR4/CXCL12 complex has already been associated with progression of atherosclerosis; however, its exact role is yet unknown. The aim of this study was to analyse the expression and cellular localization of CXCL12 and its receptor CXCR4 in human carotid atherosclerotic plaques. Methods Carotid plaques (n = 58; 31 stable, 27 unstable, based on histological characterization of plaque morphology) were obtained during carotid endarterectomy, and 10 healthy vessels were used as a control. Expression of cxcr4, cxcr7, cxcl12, ccl2/ccr2 and csf1/csf1r was analysed at mRNA, and level expression of CXCR4, CXCR7 and CXCL12 was analysed at protein level. Cellular localization was determined using consecutive and double immunohistochemical (IHC) staining and microdissection. Results At mRNA level, cxcr4, cxcr7 and cxcl12 were significantly higher expressed in stable carotid plaques compared with controls (p = 0.011, p < 0.001 and p < 0.001). Cxcl12 mRNA expression was successively augmented toward unstable plaques (p < 0.001). At protein level, CXCR4, CXCR7 and CXCL12 expression was significantly increased in both stable (p = 0.001, p < 0.001 and p = 0.035, respectively) and unstable (p = 0.003, p < 0.001 and p = 0.045, respectively) plaques compared with controls. Using IHC, CXCR4 was particularly localized in macrophages and small neovessels. Microdissection confirmed strongest expression of cxcr4 in macrophages within atherosclerotic plaques. Leukocytes and smooth muscle cells showed cxcr4 expression as well. For cxcl12, only microdissected areas with macrophages were positive. Conclusion Expression of CXCR4 and CXCL12 was significantly increased in both stable and unstable carotid atherosclerotic plaques compared with healthy vessels, both at mRNA and protein level. CXCR4 and CXCL12 were localized particularly in macrophages.

Near-infrared spectroscopy

ESC CardioMed ◽  
2018 ◽  
pp. 637-640
Author(s):  
Carlo Di Mario ◽  
Carlotta Sorini Dini ◽  
Serafina Valente
Keyword(s):  
Infrared Spectroscopy ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Local Treatment ◽  
Atherosclerotic Plaques ◽  
High Lipid ◽  
Coronary Syndromes ◽  
St Elevation ◽  
Unstable Plaques

Near-infrared spectroscopy (NIRS) is a new intracoronary imaging technique that detects and quantifies cholesterol-rich atherosclerotic plaques. NIRS can be combined with intravascular ultrasound to provide morphological information together with a chemogram of the atherosclerotic plaques. This technique has been used for characterization of unstable plaques, showing a nearly universal presence of high lipid content in patients with ST elevation myocardial infarction or acute coronary syndromes, for prediction of embolization risk and for assessing the effects of cholesterol-lowering therapy. The main potential advantage of NIRS is the identification of vulnerable plaques at high risk, to be targeted by local treatment and more aggressive preventive measures.

The Use of Multidimensional Microscopy in the Characterization of Retinal Vascular Development in a Murine Model

2001 ◽  
Vol 7 (S2) ◽  
pp. 1010-1011
Author(s):  
Robert G. Summers ◽  
Edith Aguilar ◽  
Marilyn Leonard ◽  
Martin Friedlander
Keyword(s):  
Collagen Type ◽  
Vascular Development ◽  
Multiphoton Microscopy ◽  
Vascular Diseases ◽  
Collagen Type Iv ◽  
Retinal Vasculature ◽  
3 Dimensional ◽  
Type Iv ◽  
Developing System

The murine retinal vasculature develops in its entirety following birth through the process of angiogenesis. This developing system provides an extremely accessible model for investigation of angiogenic mechanisms in general and as they pertain to vascular diseases of the eye (e.g. diabetic retinopathy and macular degeneration). The model is particularly relevant clinically because the developmental events of retinal vascularization are nearly identical in mice and humans, although primate vessels develop in utero during the final trimester of pregnancy and are thus less accessible for experimental study. The model is also excellent for the testing of anti-angiogenic agents as potential treatments for vascular diseases. in this report we outline the use of confocal optical microscopy and multiphoton microscopy in the 3-dimensional characterization of retinal vascular development in the mouse.We employed confocal microscopy to characterize the events of vascular development from postnatal days 0-56 (P0-P56). During this time the retinas are transformed from simple, thin bilaminar structures to more complex, functional, adult sensory organs. Retinal morphogenesis continues until P42. Retinas were dissected from eyes and vessels stained with collagen type-IV antibody (1) or Bandeiraea simplicifolia (Griffonia) lectin-dye conjugate (2) to outline the blood vessels. All patent vessels were stained by both techniques.

scholarly journals Fluorescence lifetime imaging for the characterization of the biochemical composition of atherosclerotic plaques

2011 ◽  
Vol 16 (9) ◽  
pp. 096018 ◽  
Author(s):  
Jennifer Phipps ◽  
Yinghua Sun ◽  
Ramez Saroufeem ◽  
Nisa Hatami ◽  
Michael C. Fishbein ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Biochemical Composition ◽  
Atherosclerotic Plaques ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging
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