scholarly journals Selection of biochemical mutants of Aspergillus niger resistant to some abiotic stresses with increased inulinase production

2003 ◽  
Vol 95 (4) ◽  
pp. 686-692 ◽  
Author(s):  
M. Skowronek ◽  
J. Fiedurek
Keyword(s):  
Aspergillus Niger ◽  
Abiotic Stresses ◽  
Selection Of

scholarly journals The Lipomyces starkeyi gene Ls120451 encodes a cellobiose transporter that enables cellobiose fermentation in Saccharomyces cerevisiae

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Jorg C de Ruijter ◽  
Kiyohiko Igarashi ◽  
Merja Penttilä
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Niger ◽  
Heterologous Expression ◽  
In Silico ◽  
Penicillium Oxalicum ◽  
Microbial Fermentation ◽  
Lipomyces Starkeyi ◽  
Yeast Saccharomyces Cerevisiae ◽  
Sugar Transporters ◽  
Selection Of

ABSTRACT Processed lignocellulosic biomass is a source of mixed sugars that can be used for microbial fermentation into fuels or higher value products, like chemicals. Previously, the yeast Saccharomyces cerevisiae was engineered to utilize its cellodextrins through the heterologous expression of sugar transporters together with an intracellular expressed β-glucosidase. In this study, we screened a selection of eight (putative) cellodextrin transporters from different yeast and fungal hosts in order to extend the catalogue of available cellobiose transporters for cellobiose fermentation in S. cerevisiae. We confirmed that several in silico predicted cellodextrin transporters from Aspergillus niger were capable of transporting cellobiose with low affinity. In addition, we found a novel cellobiose transporter from the yeast Lipomyces starkeyi, encoded by the gene Ls120451. This transporter allowed efficient growth on cellobiose, while it also grew on glucose and lactose, but not cellotriose nor cellotetraose. We characterized the transporter more in-depth together with the transporter CdtG from Penicillium oxalicum. CdtG showed to be slightly more efficient in cellobiose consumption than Ls120451 at concentrations below 1.0 g/L. Ls120451 was more efficient in cellobiose consumption at higher concentrations and strains expressing this transporter grew slightly slower, but produced up to 30% more ethanol than CdtG.

scholarly journals Screening economically important features of spring common wheat varieties of CIMMYT breeding in the central Russia

2021 ◽  
Vol 845 (1) ◽  
pp. 012065
Author(s):  
V S Rubets ◽  
V V Pylnev ◽  
I N Voronchihina ◽  
Yu N Kotenko ◽  
D D Taranova
Keyword(s):  
Common Wheat ◽  
Abiotic Stresses ◽  
Grain Quality ◽  
Wheat Variety ◽  
Genotypic Diversity ◽  
Biotic And Abiotic Stresses ◽  
Wheat Varieties ◽  
Central Russia ◽  
Selection Of ◽  
Spring Common Wheat

Abstract Creating of populations with wide genotypic diversity is the basis of successful selection of promising lines. This can be achieved by involving breeding material from various ecological and geographical origins, as well as interspecific hybrids, in crossing. 14 spring common wheat (Triticum aestivum L.) varieties of CIMMYT breeding (Mexico) were researched to identify the genetic sources of economically important features. A number of these varieties proved to be the sources of adaptability to biotic and abiotic stresses and high grain quality. The yield properties of the Mexican varieties in the central Russia were at or below the standard – the ‘Zlata ’ wheat variety.

scholarly journals Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents

2019 ◽  
Vol 7 (8) ◽  
pp. 249 ◽  
Author(s):  
Houda Ben Slama ◽  
Mohamed Ali Triki ◽  
Ali Chenari Bouket ◽  
Fedia Ben Mefteh ◽  
Faizah N. Alenezi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Abiotic Stresses ◽  
Plant Pathogens ◽  
Metal Removal ◽  
Plant Protection ◽  
Biocontrol Agents ◽  
In Planta ◽  
Olive Trees ◽  
Limoniastrum Monopetalum ◽  
Selection Of

Halophyte Limoniastrum monopetalum, an evergreen shrub inhabiting the Mediterranean region, has well-documented phytoremediation potential for metal removal from polluted sites. It is also considered to be a medicinal halophyte with potent activity against plant pathogens. Therefore, L. monopetalum may be a suitable candidate for isolating endophytic microbiota members that provide plant growth promotion (PGP) and resistance to abiotic stresses. Selected for biocontrol abilities, these endophytes may represent multifaceted and versatile biocontrol agents, combining pathogen biocontrol in addition to PGP and plant protection against abiotic stresses. In this study 117 root culturable bacterial endophytes, including Gram-positive (Bacillus and Brevibacillus), Gram-negative (Proteus, Providencia, Serratia, Pantoea, Klebsiella, Enterobacter and Pectobacterium) and actinomycete Nocardiopsis genera have been recovered from L. monopetalum. The collection exhibited high levels of biocontrol abilities against bacterial (Agrobacterium tumefaciens MAT2 and Pectobacterium carotovorum MAT3) and fungal (Alternaria alternata XSZJY-1, Rhizoctonia bataticola MAT1 and Fusarium oxysporum f. sp. radicis lycopersici FORL) pathogens. Several bacteria also showed PGP capacity and resistance to antibiotics and metals. A highly promising candidate Bacillus licheniformis LMRE 36 with high PGP, biocontrol, metal and antibiotic, resistance was subsequently tested in planta (potato and olive trees) for biocontrol of a collection of 14 highly damaging Fusarium species. LMRE 36 proved very effective against the collection in both species and against an emerging Fusarium sp. threatening olive trees culture in nurseries. These findings provide a demonstration of our pyramiding strategy. Our strategy was effective in combining desirable traits in biocontrol agents towards broad-spectrum resistance against pathogens and protection of crops from abiotic stresses. Stacking multiple desirable traits into a single biocontrol agent is achieved by first, careful selection of a host for endophytic microbiota recovery; second, stringent in vitro selection of candidates from the collection; and third, application of the selected biocontrol agents in planta experiments. That pyramiding strategy could be successfully used to mitigate effects of diverse biotic and abiotic stresses on plant growth and productivity. It is anticipated that the strategy will provide a new generation of biocontrol agents by targeting the microbiota of plants in hostile environments.

scholarly journals Suitability ofVaderfor Transposon-Mediated Mutagenesis inAspergillus niger

2011 ◽  
Vol 77 (7) ◽  
pp. 2332-2336 ◽  
Author(s):  
Elkbir Hihlal ◽  
Ilka Braumann ◽  
Marco van den Berg ◽  
Frank Kempken
Keyword(s):  
Aspergillus Niger ◽  
Filamentous Fungus ◽  
Transposon Mutagenesis ◽  
Transposon Tagging ◽  
Biotechnological Applications ◽  
Excision Event ◽  
Vector Construction ◽  
Tail Pcr ◽  
Selection Of

ABSTRACTThe filamentous fungusAspergillus nigeris widely used in biotechnological applications. Strain CBS513.88 is known to harbor 21 copies of the nonautonomous transposonVader. Upon selection of chlorate-resistantA. nigercolonies, oneVadercopy was found integrated in thenirAgene. This copy was used for vector construction and development of a transposon-tagging method.Vadershowed an excision frequency of about 1 in 2.2 × 105conidiospores. A total of 95 of 97 colonies analyzed exhibited an excision event at the DNA level, andVaderfootprints were found. By employing thermal asymmetric interlaced (TAIL)-PCR, the reintegration sites of 21 independent excision events were determined. All reintegration events occurred within or very close to genes. Therefore, this method can be used for transposon mutagenesis inA. niger.

Selection of candidate reference genes for real-time PCR studies in lettuce under abiotic stresses

Planta ◽  
2014 ◽  
Author(s):  
Joyce Moura Borowski ◽  
Vanessa Galli ◽  
Rafael da Silva Messias ◽  
Ellen Cristina Perin ◽  
Julieti Hugh Buss ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Abiotic Stresses ◽  
Reference Genes ◽  
Selection Of

Selection of amylolytically active Aspergillus niger mutants to 2-deoxy-D-glucose

1987 ◽  
Vol 142 (5) ◽  
pp. 407-412 ◽  
Author(s):  
J. Fiedurek ◽  
A. Paszczyński ◽  
G. Ginalska ◽  
Z. Ilczuk
Keyword(s):  
Aspergillus Niger ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document