Angiotensin II type 1 receptor blockade restores angiotensin-(1–7)-induced coronary vasodilation in hypertrophic rat hearts

2013 ◽  
Vol 125 (9) ◽  
pp. 449-459 ◽  
Author(s):  
Álvaro P. S. Souza ◽  
Deny B. S. Sobrinho ◽  
Jônathas F. Q. Almeida ◽  
Gisele M. M. Alves ◽  
Larissa M. Macedo ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Receptor Antagonist ◽  
At1 Receptor ◽  
At2 Receptor ◽  
Receptor Blockade ◽  
Coronary Vasodilation ◽  
Mas Receptor ◽  
Rat Hearts ◽  
Pre Treatment

The aim of the present study was to investigate the coronary effects of Ang-(1–7) [angiotensin-(1–7)] in hypertrophic rat hearts. Heart hypertrophy was induced by abdominal aorta CoA (coarctation). Ang-(1–7) and AVE 0991, a non-peptide Mas-receptor agonist, at picomolar concentration, induced a significant vasodilation in hearts from sham-operated rats. These effects were blocked by the Mas receptor antagonist A-779. Pre-treatment with L-NAME (NG-nitro-L-arginine methyl ester) or ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinozalin-1-one) [NOS (NO synthase) and soluble guanylate cyclase inhibitors respectively] also abolished the effect of Ang-(1–7) in control hearts. The coronary vasodilation produced by Ang-(1–7) and AVE 0991 was completely blunted in hypertrophic hearts. Chronic oral administration of losartan in CoA rats restored the coronary vasodilation effect of Ang-(1–7). This effect was blocked by A-779 and AT2 receptor (angiotensin II type 2 receptor) antagonist PD123319. Acute pre-incubation with losartan also restored the Ang-(1–7)-induced, but not BK (bradykinin)-induced, coronary vasodilation in hypertrophic hearts. This effect was inhibited by A-779, PD123319 and L-NAME. Chronic treatment with losartan did not change the protein expression of Mas and AT2 receptor and ACE (angiotensin-converting enzyme) and ACE2 in coronary arteries from CoA rats, but induced a slight increase in AT2 receptor in aorta of these animals. Ang-(1–7)-induced relaxation in aortas from sham-operated rats was absent in aortas from CoA rats. In vitro pre-treatment with losartan restored the Ang-(1–7)-induced relaxation in aortic rings of CoA rats, which was blocked by the Mas antagonist A-779 and L-NAME. These data demonstrate that Mas is strongly involved in coronary vasodilation and that AT1 receptor (angiotensin II type 1 receptor) blockade potentiates the vasodilatory effects of Ang-(1–7) in the coronary beds of pressure-overloaded rat hearts through NO-related AT2- and Mas-receptor-dependent mechanisms. These data suggest the association of Ang-(1–7) and AT1 receptor antagonists as a potential therapeutic avenue for coronary artery diseases.

Postischemic apoptosis and functional recovery after angiotensin II type 1 receptor blockade in isolated working rat hearts

2001 ◽  
Vol 19 (6) ◽  
pp. 1121-1129 ◽  
Author(s):  
Rohit Moudgil ◽  
Vijayan Menon ◽  
Yi Xu ◽  
Sorin Musat-Marcu ◽  
Dinender Kumar ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Functional Recovery ◽  
Receptor Blockade ◽  
Rat Hearts

scholarly journals Constitutively Active Mutant N111G of Angiotensin II Type 1 (AT1) Receptor Induces Homologous Internalization Through Mediation of AT1-Receptor Antagonist

2009 ◽  
Vol 111 (3) ◽  
pp. 227-234 ◽  
Author(s):  
Mohiuddin Ahmed Bhuiyan ◽  
Murad Hossain ◽  
Shin-ichiro Miura ◽  
Takashi Nakamura ◽  
Masanobu Ozaki ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Receptor Antagonist ◽  
At1 Receptor ◽  
Constitutively Active

scholarly journals Angiotensin Ii Type 1 (at1 ) Receptor Blockade Enhances the L-NAME-Induced Vasoconstriction in Rat Submandibular Gland

2002 ◽  
Vol 87 (3) ◽  
pp. 327-333 ◽  
Author(s):  
J. Vág ◽  
Beáta Kerémi ◽  
Csilla Hably ◽  
J. Bartha ◽  
Á. Fazekas
Keyword(s):  
Angiotensin Ii ◽  
Submandibular Gland ◽  
At1 Receptor ◽  
Receptor Blockade ◽  
Rat Submandibular Gland

scholarly journals Angiotensin II Dilates Bovine Adrenal Cortical Arterioles: Role of Endothelial Nitric Oxide

Endocrinology ◽  
2005 ◽  
Vol 146 (8) ◽  
pp. 3319-3324 ◽  
Author(s):  
Kathryn M. Gauthier ◽  
David X. Zhang ◽  
Erik M. Edwards ◽  
Blythe Holmes ◽  
William B. Campbell
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cell ◽  
Angiotensin Ii ◽  
Receptor Antagonist ◽  
Receptor Activation ◽  
At1 Receptor ◽  
Internal Diameter ◽  
No Production ◽  
At2 Receptor ◽  
Angiotensin Type

Abstract Adrenal steroidogenesis is modulated by humoral and neuronal factors and blood flow. Angiotensin II (AII) stimulates adrenal cortical aldosterone and cortisol production and medullary catecholamine release. However, AII regulation of adrenal vascular tone has not been characterized. We examined the effect of AII on diameters of cannulated bovine adrenal cortical arteries. Cortical arteries (average internal diameter = 230 μm) were constricted with U46619 and concentration-diameter responses to AII (10−13 to 10−8 mol/liter) were measured. In endothelium-intact arteries, AII induced dilations at low concentrations (maximum dilation = 25 ± 6% at 10−10 mol/liter) and constrictions at high concentrations (maximum constriction = 25 ± 18% at 10−8 mol/liter). AII constrictions were blocked by the angiotensin type 1 (AT1) receptor antagonist, losartan (10−6 mol/liter). AII dilations were enhanced by losartan (maximal dilation = 48 ± 8%), abolished by endothelial cell removal or N-nitro-l-arginine (L-NA, 3 × 10−5 mol/liter) and inhibited by the angiotensin type 2 (AT2) receptor antagonist, PD123319 (10−6 mol/liter, maximal dilation = 18 ± 4%). In a 4,5-diaminofluorescein diacetate nitric oxide (NO) assay of isolated cortical arteries, AII stimulated NO production, which was abolished by PD123319, L-NA, or endothelial cell removal. Western immunoblot of arterial homogenates and endothelial and zona glomerulosa cell lysates revealed 48-kD and 50-kD bands corresponding to AT1 and AT2 receptors, respectively, in all three and a 140-kD band corresponding to endothelial NO synthase in endothelial cells and arteries. Our results demonstrate that AII stimulates adrenal cortical arterial dilation through endothelial cell AT2 receptor activation and NO release and AT1 receptor-dependent constriction.

scholarly journals Effects of YM358, an Angiotensin II Type 1(AT1) Receptor Antagonist, and Enalapril on Blood Pressure and Vasoconstriction in Two Renal Hypertension Models.

2000 ◽  
Vol 23 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Tomoko TOKIOKA ◽  
Masayuki SHIBASAKI ◽  
Akira FUJIMORI ◽  
Yasuko MATSUDA-SATOH ◽  
Wataru UCHIDA ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Angiotensin Ii ◽  
Receptor Antagonist ◽  
Renal Hypertension ◽  
At1 Receptor

scholarly journals Impaired Vascular Contractility and Aortic Wall Degeneration in Fibulin-4 Deficient Mice: Effect of Angiotensin II Type 1 (AT1) Receptor Blockade

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23411 ◽  
Author(s):  
Els Moltzer ◽  
Luuk te Riet ◽  
Sigrid M. A. Swagemakers ◽  
Paula M. van Heijningen ◽  
Marcel Vermeij ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Aortic Wall ◽  
At1 Receptor ◽  
Receptor Blockade ◽  
Vascular Contractility ◽  
Deficient Mice
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