Fatty acids and cytokine mRNA expression in human osteoblastic cells: a specific effect of arachidonic acid

2002 ◽  
Vol 102 (4) ◽  
pp. 403 ◽  
Author(s):  
G. PRIANTE ◽  
L. BORDIN ◽  
E. MUSACCHIO ◽  
G. CLARI ◽  
B. BAGGIO
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Mrna Expression ◽  
Specific Effect ◽  
Osteoblastic Cells ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression ◽  
Human Osteoblastic Cells

Fatty acids and cytokine mRNA expression in human osteoblastic cells: a specific effect of arachidonic acid

2002 ◽  
Vol 102 (4) ◽  
pp. 403-409 ◽  
Author(s):  
G. PRIANTE ◽  
L. BORDIN ◽  
E. MUSACCHIO ◽  
G. CLARI ◽  
B. BAGGIO
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Mrna Expression ◽  
Specific Effect ◽  
Significant Inhibition ◽  
Cytokine Gene Expression ◽  
Dependent Manner ◽  
Human Osteoblast ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

Epidemiological, clinical and experimental evidence suggests that fatty acids have a modulatory effect on bone metabolism in animals and humans. To investigate this hypothesis, we evaluated the effects of three different fatty acids, arachidonic acid (AA), eicosapentaenoic acid (EPA) and oleic acid (OA), on the expression of cytokines involved in bone remodelling. Cytokine mRNAs in the human osteoblast-like cell line MG-63 were quantified by reverse transcription-PCR. AA induced increased expression of interleukin-1α, interleukin-1β, tumour necrosis factor-α and macrophage colony-stimulating factor mRNAs in a time- and dose-dependent manner. EPA and OA had no stimulatory effects, but instead caused a significant inhibition of AA-induced cytokine mRNA expression. Cell treatment with calphostin C, an inhibitor of protein kinase C (PKC), and cellular PKC down-regulation experiments independently resulted in significant inhibition of AA-induced cytokine expression, suggesting that a PKC-dependent mechanism accounts for the effects of AA on cytokine production. In conclusion, our study demonstrates specific effects of fatty acids on cytokine gene expression in human osteoblast-like cells. The clinical relevance of our findings requires further investigation.

scholarly journals Cytokine mRNA Expression After Exposure to Fatty Acids is Differently Modulated in Macrophages From Small Intestine and Colon

2011 ◽  
Vol 140 (5) ◽  
pp. S-860
Author(s):  
Chie Kurihara ◽  
Ryota Hokari ◽  
Masaaki Higashiyama ◽  
Toshihide Ueda ◽  
Hideaki Hozumi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Small Intestine ◽  
Mrna Expression ◽  
Cytokine Mrna ◽  
Cytokine Mrna Expression

Specific effect of arachidonic acid on inducible nitric oxide synthase mRNA expression in human osteoblastic cells

2005 ◽  
Vol 109 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Giovanna Priante ◽  
Estella Musacchio ◽  
Elisa Pagnin ◽  
Lorenzo A. Calò ◽  
Bruno Baggio
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Arachidonic Acid ◽  
Mrna Expression ◽  
Inos Expression ◽  
Osteoblastic Cells ◽  
Human Osteoblast ◽  
Inos Gene ◽  
Human Osteoblastic Cells ◽  
Inos Gene Expression

A specific modulatory effect of PUFAs (polyunsaturated fatty acids) on gene expression of some cytokines involved in bone remodelling has been reported previously. In particular, although a direct action of AA (arachidonic acid) on bone cytokine gene expression has been shown in human osteoblastic cells, OA (oleic acid) and EPA (eicosapentaenoic acid) were ineffective. Since the NO (nitric oxide) system has also been shown to have an important modulatory activity on osteoblasts, osteoclasts and bone metabolism, in the present study we have investigated the effects of PUFAs on iNOS (inducible NO synthase) gene expression in a human osteoblast-like cell line. AA induced a significant increase in iNOS mRNA expression, whereas EPA and OA had no stimulatory effects but instead caused a significant inhibition of AA-induced iNOS gene expression. Blocking of the COX (cyclo-oxygenase) pathway did not inhibit AA-induced iNOS expression. AA action was inhibited instead by the addition of calphostin C and genistein, inhibitors of PKC (protein kinase C) and tyrosine kinases respectively. Experiments performed with specific anti-cytokine antibodies showed a significant decrease in iNOS expression in AA-treated osteoblastic cells, suggesting that both cytokine-dependent and -independent mechanisms account for the effects of AA on iNOS gene expression. In conclusion, our investigation clearly shows specific effects of PUFAs on iNOS expression in human osteoblast-like cells with a cytokine-dependent and -independent mechanism. These results might have clinical relevance and are of interest for understanding the reported beneficial effects of dietary PUFA manipulation on the prevention and/or treatment of primary and secondary bone disease.

CYTOKINE mRNA EXPRESSION IN HUMAN PLATELETS AND A MEGAKARYOCYTIC CELL LINE AND CYTOKINE MODULATION OF PLATELET FUNCTION

Cytokine ◽  
1997 ◽  
Vol 9 (6) ◽  
pp. 405-411 ◽  
Author(s):  
Gerald Soslau ◽  
Doris A. Morgan ◽  
Jonathan S. Jaffe ◽  
Isadore Brodsky ◽  
Yihe Wang
Keyword(s):  
Mrna Expression ◽  
Cell Line ◽  
Platelet Function ◽  
Human Platelets ◽  
Cytokine Mrna ◽  
Cytokine Modulation ◽  
Cytokine Mrna Expression ◽  
Megakaryocytic Cell
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