Isolation and characterization of the viscous, high-molecular-mass microbial carbohydrate fraction from faeces of healthy subjects and patients with Crohn’s disease and the consequences for a therapeutic approach

1998 ◽  
Vol 95 (4) ◽  
pp. 425
Author(s):  
Johanneke G.H. RUSELER-VAN EMBDEN ◽  
Leo M.C. VAN LIESHOUT ◽  
Doede J. BINNEMA ◽  
Maarten P. HAZENBERG
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Molecular Mass ◽  
Therapeutic Approach ◽  
Healthy Subjects ◽  
High Molecular Mass ◽  
Isolation And Characterization ◽  
Carbohydrate Fraction

Isolation and characterization of the viscous, high-molecular-mass microbial carbohydrate fraction from faeces of healthy subjects and patients with Crohn's disease and the consequences for a therapeutic approach

1998 ◽  
Vol 95 (4) ◽  
pp. 425-433
Author(s):  
Johanneke G. H. RUSELER-VAN EMBDEN ◽  
Leo M. C. VAN LIESHOUT ◽  
Doede J. BINNEMA ◽  
Maarten P. HAZENBERG
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Molecular Mass ◽  
Healthy Subjects ◽  
Mass Fraction ◽  
High Molecular Mass ◽  
Muramic Acid ◽  
Bacterial Origin ◽  
Intestinal Contents ◽  
Daily Dose

1.An earlier study by our group revealed that the viscosity of faeces from patients with Crohn's disease is significantly lower than that of healthy subjects. This is due to low concentrations of a high-molecular-mass carbohydrate, probably of bacterial origin. The cause of this phenomenon might be the impaired barrier function of the gut mucosa. Low viscosity may allow close contact of intestinal contents (bacterial products and toxins) with the intestinal wall. This could play a role in the maintenance of the disease. 2.The first aim of this study was to investigate the high-molecular-mass carbohydrate fraction, responsible for viscosity, in detail. We also tried (in a pilot study) to raise the intestinal viscosity of patients with Crohn's disease with the undegradable food additive hydroxypropylcellulose (E463), in an attempt to alleviate clinical symptoms. 3.The high-molecular-mass fraction (> 300 ;kDa) responsible for faecal viscosity was sensitive to lysozyme and contained high levels of muramic acid. It was concluded that this material consisted mainly of peptidoglycan polysaccharides and was consequently of bacterial origin. The muramic acid in material from patients with Crohn's disease was 7.5 (1.5–13.9)%, which was less than in healthy subjects [11.4 (8.5–24.1)%; P = 0.0004]. Furthermore, viscosity in material from patients with Crohn's disease was found to be half [14.9 (1.0–33.6) cP] of that found in healthy subjects [35.0 (2.7–90.7) cP; P = 0.004]. 4.A daily dose of 1 ;g of hydroxypropylcellulose caused an increase in faecal viscosity in patients with Crohn's disease (from 1.4 to 2.3 cP) and in healthy subjects (from 4.9 to 7.5 cP). Faecal consistency improved in patients with Crohn's disease (from watery and loose to formed) and the defecation frequency decreased from 3–4 to about 2 times a day. No changes in defecation patterns were found in healthy subjects. 5.These data indicate that the high-molecular-mass fraction that is responsible for faecal viscosity is peptidoglycan. Furthermore, a daily dose of a hydroxypropylcellulose solution to increase the viscosity of the intestinal contents of patients with Crohn's disease might be beneficial. This approach merits further study.

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2011 ◽  
Vol 166 (1) ◽  
pp. 72-79 ◽  
Author(s):  
K. Mitsuyama ◽  
M. Niwa ◽  
J. Masuda ◽  
K. Kuwaki ◽  
H. Yamasaki ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Short Peptide ◽  
Isolation And Characterization

Isolation and Characterization of High-Molecular Mass DNA from Hair Shafts

1988 ◽  
Vol 369 (1) ◽  
pp. 413-416 ◽  
Author(s):  
Jochen KALBE ◽  
Rolf KUROPKA ◽  
L. Sebastian MEYER-STORK ◽  
Sybille L. SAUTER ◽  
Peter LOSS ◽  
...  
Keyword(s):  
Molecular Mass ◽  
High Molecular Mass ◽  
Isolation And Characterization ◽  
Hair Shafts

scholarly journals Metagenomic Characterization of Microbial Communities In Situ Within the Deeper Layers of the Ileum in Crohn’s Disease

2016 ◽  
Vol 2 (5) ◽  
pp. 563-566.e5 ◽  
Author(s):  
Chandra Sekhar Pedamallu ◽  
Ami S. Bhatt ◽  
Susan Bullman ◽  
Sharyle Fowler ◽  
Samuel S. Freeman ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Microbial Communities

Sa1806 Molecular and Functional Characterization of Fimh in Crohn's Disease Associated E. Coli

2013 ◽  
Vol 144 (5) ◽  
pp. S-310
Author(s):  
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Belgin Dogan ◽  
Ellen J. Scherl ◽  
Kenneth W. Simpson
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Functional Characterization ◽  
E Coli
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