Rat hepatic stellate cell expression of α2-macroglobulin is a feature of cellular activation: implications for matrix remodelling in hepatic fibrosis

1998 ◽  
Vol 95 (2) ◽  
pp. 179 ◽  
Author(s):  
C.A. KAWSER ◽  
J.P. IREDALE ◽  
P.J. WINWOOD ◽  
M.J.P. ARTHUR
Keyword(s):  
Hepatic Fibrosis ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Cellular Activation ◽  
Α2 Macroglobulin ◽  
Cell Expression

Rat hepatic stellate cell expression of α2-macroglobulin is a feature of cellular activation: implications for matrix remodelling in hepatic fibrosis

1998 ◽  
Vol 95 (2) ◽  
pp. 179-186 ◽  
Author(s):  
C. A. KAWSER ◽  
J. P. IREDALE ◽  
P. J. WINWOOD ◽  
M. J. P. ARTHUR
Keyword(s):  
Protease Inhibitor ◽  
Hepatic Fibrosis ◽  
Hepatic Stellate Cell ◽  
Hepatic Stellate Cells ◽  
Stellate Cell ◽  
Stellate Cells ◽  
Northern Analysis ◽  
Control Value ◽  
Α2 Macroglobulin ◽  
Cell Expression

1.Hepatic stellate cells are key mediators of hepatic fibrosis. We have studied hepatic stellate cell expression of the collagenase and general protease inhibitor α2-macroglobulin after activation in tissue culture and in response to certain cytokines. 2.Hepatic stellate cells isolated by Pronase–collagenase digestion were activated by culture on uncoated plastic. By Northern analysis hepatic stellate cells undergoing activation (5 days) expressed α2-macroglobulin mRNA and α2-macroglobulin could be immunolocalized to hepatic stellate cells from 5 to 15 days of culture. 3.By ELISA of cell culture supernatants hepatic stellate cell secretion of α2-macroglobulin was found to increase from 2.78±1.13 ;ng·ml-1·μg-1 DNA per 24 ;h at 5 days of culture (n = 8) to 13.55±4.64 ;ng·ml-1·μg-1 DNA per 24 ;h at 15 days of culture (n = 7). Stimulation of hepatic stellate cells with interleukin-6 at 5 days caused a significant increase in α2-macroglobulin expression as did exposure to Kupffer-cell conditioned medium. However, exposure of hepatic stellate cells to interleukin-1, transforming growth factor-β1 and tumour necrosis factor-α had no significant effect. 4.During profibrotic liver injury plasma α2-macroglobulin levels were found to increase to between 850% and 250% of the control value (100%) after bile duct ligation (72 ;h to 13 days respectively), and to 1166% and 1106% of the control value during progressive CCl4-induced fibrosis (24 ;h to 4 weeks respectively). 5.These data suggest that hepatic stellate cells are a potential source of the potent protease inhibitor α2-macroglobulin, expression of which may inhibit matrix remodelling during progressive fibrosis.

scholarly journals Expression of leptin in hepatic fibrosis and its relation with hepatic stellate cell activation

2009 ◽  
Vol 17 (21) ◽  
pp. 2127
Author(s):  
Jing Xu ◽  
Jing Zhu ◽  
Liang Pan ◽  
Jing-Xian Lu ◽  
Ming-Bing Xiao ◽  
...  
Keyword(s):  
Hepatic Fibrosis ◽  
Hepatic Stellate Cell ◽  
Cell Activation ◽  
Stellate Cell ◽  
Hepatic Stellate Cell Activation

scholarly journals Periostin promotes hepatic fibrosis in mice by modulating hepatic stellate cell activation via αv integrin interaction

2016 ◽  
Vol 51 (12) ◽  
pp. 1161-1174 ◽  
Author(s):  
Akiko Sugiyama ◽  
Keishi Kanno ◽  
Norihisa Nishimichi ◽  
Shoichiro Ohta ◽  
Junya Ono ◽  
...  
Keyword(s):  
Hepatic Fibrosis ◽  
Hepatic Stellate Cell ◽  
Cell Activation ◽  
Stellate Cell ◽  
Hepatic Stellate Cell Activation

Fungus thielavia minor blocks hepatic stellate cell proliferation and inhibits experemental hepatic fibrosis in rats

1998 ◽  
Vol 114 ◽  
pp. A1348
Author(s):  
H. Sugawara ◽  
T. Ueno ◽  
S. Tamaki ◽  
M. Sakamoto ◽  
T. Torimura ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Hepatic Fibrosis ◽  
Hepatic Stellate Cell ◽  
Stellate Cell
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