Transgenic expression of human complement regulators reduces skeletal muscle ischaemia/reperfusion injury in mice

2008 ◽  
Vol 115 (8) ◽  
pp. 263-263
Author(s):  
Kenneth R. Knight ◽  
Trixie A. Shinkel ◽  
Peter J. Cowan ◽  
Rosalind Romeo-Meeuw ◽  
Anthony J. F. d'Apice ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Reperfusion Injury ◽  
Human Complement ◽  
Ischaemia Reperfusion Injury ◽  
Transgenic Expression ◽  
Ischaemia Reperfusion ◽  
Complement Regulators ◽  
Muscle Ischaemia

Activated protein C attenuates skeletal muscle ischaemia reperfusion injury.

2003 ◽  
Vol 114 (2) ◽  
pp. 248
Author(s):  
J.P. Dillon ◽  
A.J. Laing ◽  
R.C. Cahill ◽  
G.C. O’Brien ◽  
J.H. Wang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Reperfusion Injury ◽  
Protein C ◽  
Activated Protein C ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion ◽  
Muscle Ischaemia

scholarly journals Demonstration of the temporal evolution of serum markers of skeletal muscle ischaemia reperfusion injury - An observational clinical study.

2020 ◽  
Author(s):  
THUSHAN DHANANJA GOONERATNE ◽  
Shervanthi Homer-Vanniasinkam ◽  
Serosha Mandika Wijeyaratne
Keyword(s):  
Skeletal Muscle ◽  
Reperfusion Injury ◽  
Temporal Evolution ◽  
Serum Markers ◽  
Tnf Alpha ◽  
Surrogate Markers ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion ◽  
Baseline Values ◽  
Muscle Ischaemia

Abstract Objective The study aimed to investigate the temporal evolution of markers of skeletal muscle ischaemia reperfusion injury (IRI) to shed more light into its pathogenesis towards finding a clinical therapeutic intervention. P-selectin, Myeloperoxidase and TNF-alpha were selected as markers of IRI based on their role in its pathogenesis. An observational study was conducted on patients with skeletal muscle ischaemia requiring revascularization. Loco-regional venous sampling was performed at 0, +2, +4, +6, +12 and +24 hours post-reperfusion to demonstrate the temporal evolution of surrogate markers of IRI.Results Levels of P-Selectin, Myeloperoxidase and TNF-alpha demonstrated a significant peak elevation during 2h - 4h following reperfusion when compared to baseline values. (P-selectin 59.48pg/ml to 125.91pg/ml, p=0.008; Myeloperoxidase 78.84pg/ml to 116.86pg/ml, p=0.051; TNF-alpha 1.80pg/ml to 34.53pg/ml, p=0.002) All three markers gradually subsided with time and reached baseline pre-perfusion values between 6h – 8h from time of reperfusion. The study suggests that skeletal muscle IRI in humans is associated with an early exaggerated pro-inflammatory response, as supported by increased expression of reperfusion injury markers in the venous effluent. They demonstrate potential for use as markers of IRI for future clinical trials.

Riluzole protects against skeletal muscle ischaemia-reperfusion injury in a porcine model

Injury ◽  
2020 ◽  
Vol 51 (2) ◽  
pp. 178-184 ◽  
Author(s):  
Rachel W. Li ◽  
Yi Deng ◽  
Hai Nam Pham ◽  
Steven Weiss ◽  
Mingming Chen ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Reperfusion Injury ◽  
Porcine Model ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion ◽  
Muscle Ischaemia

scholarly journals Effect of different doses of pregabalin on skeletal muscle ischaemia-reperfusion injury in rats

2017 ◽  
Vol 118 (07) ◽  
pp. 417-422
Author(s):  
L. Ozturk ◽  
H. T. Dogan ◽  
A. Kilicarslan ◽  
M. E. Aydin ◽  
A. Ozer ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Reperfusion Injury ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion ◽  
Muscle Ischaemia ◽  
Different Doses

Timing of Administration of Dexamethasone or the Nitric Oxide Synthase Inhibitor, Nitro-l-Arginine Methyl Ester, is Critical for Effective Treatment of Ischaemia-Reperfusion Injury to Rat Skeletal Muscle

1997 ◽  
Vol 93 (2) ◽  
pp. 167-174 ◽  
Author(s):  
Baimeng Zhang ◽  
Kenneth R. Knight ◽  
Bruce Dowsing ◽  
Elizabeth Guida ◽  
Long H. Phan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Methyl Ester ◽  
Nitric Oxide Synthase ◽  
Reperfusion Injury ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion ◽  
Nos Inhibitors ◽  
Oxide Synthase ◽  
Inducible Nos

1. The effects of the nitric oxide synthase (NOS) inhibitors, NG-nitro-l-arginine-methyl ester (l-NAME), nitroiminoethyl-l-ornithine and S-methylisothiourea on skeletal muscle survival following 2 h of tourniquet ischaemia and 24 h of reperfusion were compared with those of the antiinflammatory steroid, dexamethasone. 2. Administration of each of the NOS inhibitors or dexamethasone 30 min before reperfusion reduced the degree of skeletal muscle necrosis 24 h after reperfusion. 3. The influence of timing of drug administration was investigated. l-NAME administered 30 min before reperfusion, at 3 h after reperfusion, but not thereafter, significantly improved muscle survival compared with saline-treated controls. Dexamethasone administered 30 min before, or at 3 or 8 h after reperfusion, but not at 16 h, significantly improved muscle survival, but neither agent had protective effects when administered before ischaemia. 4. After 8 h of reperfusion of ischaemic skeletal muscle, cell-free homogenates contained Ca2+-independent (inducible) NOS activity which was reduced in dexamethasone-treated (2.5 mg/kg) rats. Furthermore, inducible NOS mRNA levels, as detected by reverse transcriptase-PCR, were increased after 8 h of reperfusion in saline, but not in dexamethasone-treated rats. 5. These data suggest a significant deleterious effect of endogenous NO which may be restricted to the first 3 h of the reperfusion phase of ischaemia-reperfusion injury, and raise the possibility of effective treatment of incipient reperfusion injury, even after several hours of reperfusion.

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