Reduced Skeletal Muscle Protein Content in Response to a High-Fat/Low-Carbohydrate Alcohol Diet in Male and Female Rats

2001 ◽  
Vol 101 (s45) ◽  
pp. 11P-11P
Author(s):  
RJ Hunter ◽  
KO Lindros ◽  
HA Jarvelainen ◽  
VR Preedy
Keyword(s):  
Skeletal Muscle ◽  
Protein Content ◽  
Muscle Protein ◽  
Female Rats ◽  
Skeletal Muscle Protein ◽  
High Fat ◽  
Male And Female ◽  
Male And Female Rats ◽  
Low Carbohydrate

Skeletal muscle protein synthesis and degradation exhibit sexual dimorphism after chronic alcohol consumption but not acute intoxication

2007 ◽  
Vol 292 (6) ◽  
pp. E1497-E1506 ◽  
Author(s):  
Charles H. Lang ◽  
Robert A. Frost ◽  
Thomas C. Vary
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Translation Initiation ◽  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Alcohol Intoxication ◽  
Female Rats ◽  
Chronic Alcohol ◽  
Skeletal Muscle Protein ◽  
Male And Female Rats

Epidemiological evidence suggests alcoholic myopathy is more severe in females than males, but comparable animal studies are lacking that make elucidating the biochemical locus for this defect problematic. The present study determined whether skeletal muscle protein synthesis and markers of degradation exhibit a sexual dimorphic response to either chronic alcohol consumption or acute intoxication. Male and female rats were fed an alcohol-containing diet, pair-fed for 26 wk (chronic), or received an intraperitoneal injection of alcohol (acute). In males, chronic alcohol decreased gastrocnemius protein synthesis by 20%. This reduction was associated with a twofold increase in the inactive eukaryotic initiation factor (eIF) 4E·4E-binding protein 1 (4E-BP1) complex and a 60% reduction in the active eIF4E·eIF4G complex. This redistribution of eIF4E was associated with decreased phosphorylation of both 4E-BP1 and eIF4G (50–55%). The phosphorylation of ribosomal protein S6 was also reduced 60% in alcohol-consuming male rats. In contrast, neither rates of protein synthesis nor indexes of translation initiation in muscle were altered in alcohol-fed female rats despite blood alcohol levels comparable to males. Chronic alcohol ingestion did not alter atrogin-1 or muscle RING finger-1 mRNA content (biomarkers of muscle proteolysis) in males but increased their expression in females 50–100%. Acute alcohol intoxication produced a comparable decrease in muscle protein synthesis and translation initiation in both male and female rats. Our data demonstrate a sexual dimorphism for muscle protein synthesis, translation initiation, and proteolysis in response to chronic, but not acute, alcohol intoxication; however, they do not support evidence indicating females are more sensitive toward the development of alcoholic skeletal muscle myopathy.

scholarly journals Skeletal Muscle and Liver Oxidative Metabolism in Response to a Voluntary Isocaloric Intake of a High Fat Diet in Male and Female Rats

2008 ◽  
Vol 22 (1-4) ◽  
pp. 327-336 ◽  
Author(s):  
Antoni Català-Niell ◽  
Maria Estrany ◽  
Ana Proenza ◽  
Magdalena Gianotti ◽  
Isabel Lladó
Keyword(s):  
Skeletal Muscle ◽  
Oxidative Metabolism ◽  
High Fat Diet ◽  
Female Rats ◽  
High Fat ◽  
Male And Female ◽  
Male And Female Rats

scholarly journals Alcohol Affects the Skeletal Muscle Proteins, Titin and Nebulin in Male and Female Rats

2003 ◽  
Vol 133 (4) ◽  
pp. 1154-1157 ◽  
Author(s):  
R. J. Hunter ◽  
C. Neagoe ◽  
H. A. Järveläinen ◽  
C. R. Martin ◽  
K. O. Lindros ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Female Rats ◽  
Muscle Proteins ◽  
Male And Female ◽  
Male And Female Rats ◽  
Skeletal Muscle Proteins

Cachectin/TNF or IL-1 alpha induces cachexia with redistribution of body proteins

1989 ◽  
Vol 256 (3) ◽  
pp. R659-R665 ◽  
Author(s):  
Y. Fong ◽  
L. L. Moldawer ◽  
M. Marano ◽  
H. Wei ◽  
A. Barber ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Content ◽  
Muscle Protein ◽  
Interleukin 1 ◽  
Metabolic Effects ◽  
Liver Protein ◽  
Mrna Levels ◽  
Skeletal Muscle Protein ◽  
Male Wistar Rats

Macrophage secretory products are suspected to participate in the severe lean tissue wasting related to chronic illness. The protein metabolic effects of chronic, 7-day cachectin/tumor necrosis factor (cachectin) or interleukin 1 alpha (IL-1 alpha) administration in vivo were studied in male Wistar rats that were 1) freely fed, 2) pair fed, 3) total protein and calorie starved, 4) twice daily lipopolysaccharide (LPS) administered, 5) twice daily cachectin administered, and 6) twice daily IL-1 alpha administered. LPS, cachectin, or IL-1 alpha administration produced anorexia; weight loss in these groups was comparable to respective pair-fed animals. However, LPS, cachectin, or IL-1 alpha accelerated peripheral protein wasting while preserving liver protein content, unlike the pattern in the pair-fed or starved animals in which loss of liver proteins and relative preservation of skeletal muscle protein were observed. The decrease in skeletal muscle protein content in LPS- or cytokine-treated animals was associated with coordinate decreases in muscle mRNA levels for the myofibrillar proteins myosin heavy chain, myosin light chain, actin, and in the 18S and 28S subunits of ribosomal RNA. We conclude that chronic exposure to the cytokines, IL-1 alpha or cachectin, can simulate those body and muscle protein changes seen in experimental LPS administration or chronic disease and markedly differ from the pattern of protein redistribution due to caloric restriction.

scholarly journals Long-term High-fat-diet Feeding Impairs Mitochondrial Biogenesis in Liver of Male and Female Rats

2010 ◽  
Vol 26 (3) ◽  
pp. 291-302 ◽  
Author(s):  
Antònia Nadal-Casellas ◽  
Emilia Amengual-Cladera ◽  
Ana María Proenza ◽  
Isabel Lladó ◽  
Magdalena Gianotti
Keyword(s):  
Mitochondrial Biogenesis ◽  
High Fat Diet ◽  
Female Rats ◽  
High Fat ◽  
Male And Female ◽  
Male And Female Rats

Long-term effects of high-fat diets on peroxisomal β-oxidation in male and female rats

Lipids ◽  
1985 ◽  
Vol 20 (10) ◽  
pp. 668-674 ◽  
Author(s):  
M. S. Thomassen ◽  
J. Norseth ◽  
E. N. Christiansen
Keyword(s):  
Female Rats ◽  
Long Term Effects ◽  
High Fat ◽  
Male And Female ◽  
Male And Female Rats ◽  
High Fat Diets ◽  
Fat Diets

scholarly journals The influence of dietary fibre on caecal metabolism in the rat

1983 ◽  
Vol 50 (3) ◽  
pp. 679-684 ◽  
Author(s):  
Linda F. McKay ◽  
Martin A. Eastwood
Keyword(s):  
Colonic Mucosa ◽  
Short Chain Fatty Acids ◽  
Female Rats ◽  
Bacterial Metabolism ◽  
Differential Absorption ◽  
High Fat ◽  
Male And Female ◽  
Male And Female Rats ◽  
High Fibre ◽  
Commercial Breeding

1. The influence of three diets ((1) high-fibre–low-fat, (2) low-fibre–high-fat, (3) commercial breeding diet) on the concentration of short chain fatty acids (SCFA) has been investigated in male and female rats up to 14 weeks of age.2. Hydrogen was detected in respired gas in all rats with no significant differences between diets or sex. Methane was detected only in control-fed rats at 12 weeks of age and thereafter. Caecal contents contained higher concentrations of SCFA than were found in faeces. There were no significant differences in the total faecal SCFA excreted by rats on the three diets. The proportions of SCFA in the caecum and faeces were influenced by diet.3. The production and excretion of hydrogen, methane and SCFA in the rat appeared to reflect different aspects of bacterial metabolism. Comparison of caecal and faecal SCFA may indicate differential absorption from the colon or differential metabolism by bacteria or colonic mucosa which may be influenced by diet.

scholarly journals Chronic Stress During Adolescence Blunts the Exercise-Induced Expression of Thyroid Hormone-Target Genes in Metabolically Active Tissues of Male and Female Rats

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A974-A974
Author(s):  
Marco Antonio Parra-Montes de Oca ◽  
Karen Lissette Garduño-Morales ◽  
Patricia Joseph-Bravo
Keyword(s):  
Skeletal Muscle ◽  
Thyroid Hormone ◽  
Chronic Stress ◽  
Voluntary Exercise ◽  
Female Rats ◽  
Dependent Manner ◽  
Male And Female ◽  
Male And Female Rats ◽  
Exercise Induced ◽  
Hpt Axis

Abstract Voluntary exercise activates HPT axis1, that contributes to energy mobilization and energy expenditure. Chronic stress in adulthood inhibits HPT response to voluntary wheel running in a sex dependent manner, inhibiting lipolysis of WAT2. We evaluated the effect of chronic stress during adolescence on HPT axis response to voluntary exercise in adulthood3, with emphasis on metabolic response in skeletal muscle and WAT. Wistar male and female rats (N=36 per sex) were divided in an undisturbed group (Control, C; n=18) and one chronic variable stress during adolescence group (CVS; n=18) (males: PND 30-70; females: PND 30-60). As adults (males: PND 84; females: PND: 74) rats were divided in: 1) exercise group: rats placed individually in a cage with a running wheel per 14 nights, 2) sedentary group with ad libitum feeding, 3) sedentary pair-fed group offered the same amount of food consumed by the exercised group, and kept in individual cages during 14 nights (6 rats/group). WAT weight was determined at sacrifice, hormones quantified by RIA and ELISA, gene expression by RT-PCR. Exercise-induced loss of fat mass was not detected in CVS rats. Exercise decreased corticosterone levels in C males and females of both treatments, supporting sex difference on HPA axis reprogramming by CVS. HPT axis response to voluntary exercise is attenuated by CVS also in a sex dimorphic manner: CVS decreased Trh expression in hypothalamic paraventricular nucleus and no changes in thyroid hormones concentration in males, whereas in females, slightly increased TSH, T4 and T3 levels. Sex also influenced the response of skeletal muscle and WAT to CVS. Dio2 and Pgc1a slightly increased expression in skeletal muscle of males, not of females. Adrb3 expression in WAT increased in females, but not in males; exercise-induced stimulation of Hsl expression was not observed in either sex after CVS. These results suggest that CVS imposed during rat adolescence inhibits the responses to voluntary exercise of HPT axis activity of thyroid hormone-targets in WAT and skeletal muscle in sex dependent manner. These changes could lead to reduced mobilization and the utilization of energy fuels coincident with the fatigue observed after exercise in patients with subclinical or clinical hypothyroidism. (Funded: CONACYT 284883, DGAPA IN213419)1Uribe, Endocrinology 155:2020-2030, 2014.2Parra, Front Endocrinol 10(418):1-13, 2019.3Parra, J Endocr Soc 4(Abstract Supp) Abstract SAT-451, 2020.

Hyperplastic and Early Neoplastic Lesions of the Liver in Rats of Varying Ages with Dietary-Induced Cirrhosis

1969 ◽  
Vol 55 (2) ◽  
pp. 79-83 ◽  
Author(s):  
Melvin D. Reuber
Keyword(s):  
Female Rats ◽  
High Fat ◽  
Chemical Carcinogens ◽  
Deficient Diet ◽  
Male And Female ◽  
Low Protein ◽  
Male And Female Rats ◽  
Neoplastic Lesions ◽  
Parenchymal Cells ◽  
Age And Sex

Inbred Buffalo male and female rats, 4-, 8-, 12-, 24-, and 52-weeks old, ingested a high fat, low protein and choline deficient diet. Preneoplastic and early neoplastic lesions of the parenchymal cells, as well as cirrhosis, developed in the liver. Hyperplastic lesions generally were observed more often in male animals and in younger animals. The hyperplastic lesions, developing in the periportal regions, were similar histologically and with regard to age and sex to lesions demonstrated as precarcinogenic in animals given chemical carcinogens.

scholarly journals The Effects of Eating a High Fat Diet on Sensitivity of Male and Female Rats to Methamphetamine and Dopamine D1 Receptor Agonist SKF 82958

2020 ◽  
Vol 374 (1) ◽  
pp. 6-15
Author(s):  
Jeremiah Ramos ◽  
Ethan J. Hardin ◽  
Alice H. Grant ◽  
Grace Flores-Robles ◽  
Adrian T. Gonzalez ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Receptor Agonist ◽  
Dopamine D1 Receptor ◽  
D1 Receptor ◽  
Female Rats ◽  
High Fat ◽  
Male And Female ◽  
Male And Female Rats ◽  
Skf 82958
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