Effects of Endurance Training on Mn-SOD Activity, Content, and mRNA Expression in Rat Skeletal Muscle

1994 ◽  
Vol 87 (s1) ◽  
pp. 84-85
Author(s):  
S Oh-Ishi ◽  
T Kizaki ◽  
H Yamashita ◽  
Y Ohira ◽  
Y Sato ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Endurance Training ◽  
Rat Skeletal Muscle ◽  
Sod Activity

scholarly journals Branched-chain α-keto acid dehydrogenase kinase content in rat skeletal muscle is decreased by endurance training

IUBMB Life ◽  
1998 ◽  
Vol 44 (6) ◽  
pp. 1211-1216 ◽  
Author(s):  
Hisao Fujii ◽  
Yoshiharu Shimomura ◽  
Taro Murakami ◽  
Naoya Nakai ◽  
Tasuku Sato ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Endurance Training ◽  
Keto Acid ◽  
Rat Skeletal Muscle ◽  
Acid Dehydrogenase ◽  
Branched Chain

Insulin and Contraction Directly Stimulate UCP2 and UCP3 mRNA Expression in Rat Skeletal Muscle in Vitro

2001 ◽  
Vol 283 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Steen B. Pedersen ◽  
Sten Lund ◽  
Esben S. Buhl ◽  
Bjørn Richelsen
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Rat Skeletal Muscle

scholarly journals Effect of Short-Term Endurance Exercise on COX IV and PGC-1a mRNA Expression Levels in Rat Skeletal Muscle

2019 ◽  
Vol 12 (3) ◽  
pp. 1309-1316 ◽  
Author(s):  
Nova Sylviana ◽  
Christina Natalia ◽  
Hanna Goenawan ◽  
Yuni Susanti Pratiwi ◽  
Iwan Setiawan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mrna Expression ◽  
Endurance Exercise ◽  
Mitochondrial Biogenesis ◽  
Control Group ◽  
Muscle Adaptation ◽  
Rat Skeletal Muscle ◽  
Short Term ◽  
Expression Levels ◽  
Mrna Expression Levels

Endurance exercise induces specific skeletal muscle adaptation by increasing mitochondrial oxidative phosphorylation eficiency and mitochondrial biogenesis. Many previous studies suggesting both PGC-1a and COX IV as a potential biomarker of skeletal muscle adaptation induced by exercise. But most of them only studied the effect of long-term endurance exercise, whereas the effect of short-term exercise remains unclear. To investigate short-term physiological adaptation induced by endurance exercise on expression of COX IV and PGC-1a mRNA in rat skeletal muscle. Twenty healthy male Wistar rats (Rattus norvegicus) aged 10-11 weeks old were used in this experiment. Rats were randomly assigned into 4 groups based on the time period of exercise: 1) control (C; n=5), 2) three days of exercise (E3; n=5), 3) six days of exercise (E6; n=5), 4) fifteen days of exercise (E15; n=5). The exercise groups were run at 20m/s for 30 minutes on the rat treadmill and the stationary control group was only placed inside treadmill with the machines turned off. On the last day of exercise, the rats were sacrificed then RNA from skeletal muscle was extracted. COX IV and PGC-1a mRNA expressions were measured by Reverse Transcriptase PCR. The results showed that there were statistically significant differences of PGC-1a mRNA expression levels in both soleus (F(3,16)=3.740, ps=0.033) and gastrocnemius (F(3,16)=3.969, pg=0.027) muscles. The COX IV mRNA expression levels in soleus (F(3,16)=3.801, ps=0.031) and gastrocnemius (F(3,16)=5.429, ps=0.009) muscles were also significantly increased. There were significant increases of PGC-1a and COX IV expressions in fifteen days of exercise group compared to control group in both muscles. Short-term endurance exercise induced mitochondrial biogenesis marker and mitochondrial activity marker by increasing the PGC-1a and COX IV mRNA expression levels in rat skeletal muscle significantly following the time periods of exercise.

EXERCISE TRAINING PREVENTS AGING-INDUCED DECREASE IN IRS-1 mRNA EXPRESSION IN RAT SKELETAL MUSCLE

1998 ◽  
Vol 30 (Supplement) ◽  
pp. 205
Author(s):  
M. Nagasaki ◽  
N. Nakai ◽  
Y. Shimomura ◽  
Y. Oshida ◽  
Y. Sato
Keyword(s):  
Skeletal Muscle ◽  
Exercise Training ◽  
Mrna Expression ◽  
Rat Skeletal Muscle

scholarly journals Effects of a Ketogenic Diet Containing Medium-Chain Triglycerides and Endurance Training on Metabolic Enzyme Adaptations in Rat Skeletal Muscle

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1269 ◽  
Author(s):  
Ayumi Fukazawa ◽  
Atsuko Koike ◽  
Takuya Karasawa ◽  
Momoko Tsutsui ◽  
Saki Kondo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Content ◽  
Endurance Training ◽  
Ketogenic Diet ◽  
Negative Regulator ◽  
Medium Chain Triglycerides ◽  
Rat Skeletal Muscle ◽  
Medium Chain ◽  
Coa Transferase

Long-term intake of a ketogenic diet enhances utilization of ketone bodies, a particularly energy-efficient substrate, during exercise. However, physiological adaptation to an extremely low-carbohydrate diet has been shown to upregulate pyruvate dehydrogenase kinase 4 (PDK4, a negative regulator of glycolytic flux) content in skeletal muscle, resulting in impaired high-intensity exercise capacity. This study aimed to examine the effects of a long-term ketogenic diet containing medium-chain triglycerides (MCTs) on endurance training-induced adaptations in ketolytic and glycolytic enzymes of rat skeletal muscle. Male Sprague-Dawley rats were placed on either a standard diet (CON), a long-chain triglyceride-containing ketogenic diet (LKD), or an MCT-containing ketogenic diet (MKD). Half the rats in each group performed a 2-h swimming exercise, 5 days a week, for 8 weeks. Endurance training significantly increased 3-oxoacid CoA transferase (OXCT, a ketolytic enzyme) protein content in epitrochlearis muscle tissue, and MKD intake additively enhanced endurance training–induced increases in OXCT protein content. LKD consumption substantially increased muscle PDK4 protein level. However, such PDK4 increases were not observed in the MKD-fed rats. In conclusion, long-term intake of ketogenic diets containing MCTs may additively enhance endurance training–induced increases in ketolytic capacity in skeletal muscle without exerting inhibitory effects on carbohydrate metabolism.

scholarly journals Changes in mitochondrial perilipin 3 and perilipin 5 protein content in rat skeletal muscle following endurance training and acute stimulated contraction

2015 ◽  
Vol 100 (4) ◽  
pp. 450-462 ◽  
Author(s):  
S. V. Ramos ◽  
P. C. Turnbull ◽  
R. E. K. MacPherson ◽  
P. J. LeBlanc ◽  
W. E. Ward ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Content ◽  
Endurance Training ◽  
Rat Skeletal Muscle ◽  
Perilipin 5
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