Heterogeneity of Human Gallbladder Mucin in Bile

1994 ◽  
Vol 86 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Michel J. A. van Wijland ◽  
J. Henriëtte Klinkspoor ◽  
Laurens Th. de Wit ◽  
Ronald P. J. Oude Elferink ◽  
Guido N. J. Tytgat ◽  
...  
Keyword(s):  
Blood Group ◽  
Polyclonal Antibody ◽  
Gallstone Disease ◽  
Helix Pomatia ◽  
Antigenic Determinants ◽  
Human Gallbladder ◽  
Blood Group A ◽  
Helix Pomatia Agglutinin ◽  
Significant Difference ◽  
Group A

1. Human gallbladder mucin has been implicated to play a role in gallstone disease. In spite of this fact relatively little is known about the structure of human gallbladder mucin. In this study we have investigated the possible heterogeneity of mucin. For this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. All antibodies reacted primarily with carbohydrate antigenic determinants. With these antibodies the immunoreactivity of gallbladder mucin from 60 patients with cholesterol gallstones and 20 subjects without stones was screened. In addition, reactivity with several lectins was studied. 2. Considerable heterogeneity was found with both antibody and lectin typing, but there was no significant difference in heterogeneity between mucin from patients with gallstones and control subjects. Immunoblotting revealed that there was similarity between the reaction of the polyclonal antibody and the Helix pomatia agglutinin. All mucin preparations reacting with the polyclonal antibody also bound to Helix pomatia agglutinin. Nineteen of the 21 reacting mucins (90%) were from patients with blood group A (18 patients) or AB (one patient) and expression of A antigen could be demonstrated on the mucin of these patients. The resulting two reacting mucins were from patients with type O. However, expression of the blood group antigen could not account for the lack of reactivity of the mucin of other patients. The Helix pomatia agglutinin partially blocked the reactivity of the polyclonal antibody, whereas anti-A antibody did not show inhibition, indicating that more then only blood group A epitopes were recognized by this antibody. 3. We conclude that considerable patient to patient heterogeneity of human gallbladder mucin exists. This may have functional consequences for the role of mucin in the pathogenesis of gallstone disease.

scholarly journals Association of ABO blood groups with presentation and outcomes of confirmed SARS CoV-2 infection: A prospective study in the largest COVID-19 dedicated hospital in Bangladesh

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0249252
Author(s):  
Reaz Mahmud ◽  
Mohammad Aftab Rassel ◽  
Farhana Binte Monayem ◽  
S. K. Jakaria Been Sayeed ◽  
Md Shahidul Islam ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Groups ◽  
Abo Blood Groups ◽  
Duration Of Symptoms ◽  
Presenting Symptoms ◽  
Blood Group A ◽  
Group I ◽  
Significant Difference ◽  
Group A ◽  
Group Ii

Background Globally, studies have shown conflicting results regarding the association of blood groups with SARS CoV-2 infection. Objective To observe the association between ABO blood groups and the presentation and outcomes of confirmed COVID-19 cases. Design, setting, and participants This was a prospective cohort study of patients with mild-to-moderately severe COVID-19 infections who presented in the COVID-19 unit of Dhaka Medical College Hospital and were enrolled between 01 June and 25 August, 2020. Patients were followed up for at least 30 days after disease onset. We grouped participants with A-positive and A-negative blood groups into group I and participants with other blood groups into group II. Results The cohort included 438 patients; 52 patients were lost to follow-up, five died, and 381 completed the study. The prevalence of blood group A [144 (32.9%)] was significantly higher among COVID-19 patients than in the general population (p < 0.001). The presenting age [mean (SD)] of group I [42.1 (14.5)] was higher than that of group II [38.8 (12.4), p = 0.014]. Sex (p = 0.23) and co-morbidity (hypertension, p = 0.34; diabetes, p = 0.13) did not differ between the patients in groups I and II. No differences were observed regarding important presenting symptoms, including fever (p = 0.72), cough (p = 0.69), and respiratory distress (p = 0.09). There was no significant difference in the median duration of symptoms in the two group (12 days), and conversion to the next level of severity was observed in 26 (20.6%) and 36 patients (13.8%) in group I and II, respectively. However, persistent positivity of RT-PCR at 14 days of initial positivity was more frequent among the patients in group I [24 (19%)] than among those in group II [29 (11.1%)]. Conclusions The prevalence of blood group A was higher among COVID-19 patients. Although ABO blood groups were not associated with the presentation or recovery period of COVID-19, patients with blood group A had delayed seroconversion.

scholarly journals Blood group and Human Leucocyte Antigen sub-type as determinants to keloid formation and Recurrence in Keloid Patients; A prospective longitudinal cohort study

2021 ◽  
Author(s):  
Ferdinand Nangole ◽  
Kelsey Ouyang ◽  
George Agak ◽  
Julius Ogeng'o ◽  
Anzala Omu
Keyword(s):  
Blood Group ◽  
Genetic Factors ◽  
Control Group ◽  
Prospective Longitudinal Study ◽  
Female Ratio ◽  
Blood Group A ◽  
Significant Difference ◽  
Group A ◽  
Surgical Conditions ◽  
Prospective Longitudinal

Abstract The role of genetic factors in keloid is a firmed by the fact that keloids have been shown to occur among members of the same family.. We undertook this study to determine whether there is any association between patients’ bloodgroup and HLA sub-types to keloids and keloid recurrence. This was a prospective longitudinal study of patients with keloids and a control of patients managed for other surgical conditions with no keloids. Blood was taken from each patient and analysed for blood group and HLA sub-types using the sequence specific primer geno-typing. Data captured were summarized and analysed using students T-test to compare means. Probability values significance was at 0.05. A total of 90 patients with keloids and 59 in a control group were followed up in the study. The male to female ratio of the patients was 2:1. The most common blood group for both groups was blood group O at 51.3% and 49.2%, followed by blood group A and B respectively. Patients with keloids had a significantly higher positive alleles with DQA*01, DQB1*05, DQB1*06 and DRB1*15. There was an association between blood group A and DQB1*06 and recurrence. In conclusion, this study demonstrates that there is significant difference in HLA sub-types among patients who form keloids and the non-keloid forming patients among our study cohorts. Salient differences were also noted in patients with keloid recurrence based on their blood group, a pointer to the significance of genetic factors in keloid pathogenesis and severity.

scholarly journals Association of Pre-hypertension with blood groups in young population.

2019 ◽  
Vol 26 (11) ◽  
pp. 1820-1824
Author(s):  
Benash Altaf ◽  
Shireen Jawed ◽  
Fakiha Behram ◽  
Zeeshan Ali Khan ◽  
Shakeela Naz
Keyword(s):  
Blood Group ◽  
Demographic Data ◽  
Cross Sectional Study ◽  
Blood Groups ◽  
P Value ◽  
Continuous Variables ◽  
Cross Sectional ◽  
Blood Group A ◽  
Significant Difference ◽  
Group A

Objectives: Pakistan has highest mortality rate due to hypertension and its complications. Hypertension is a squealed of Pre- hypertension which is believed to start at adolescent and continue to adulthood. Association of blood group with hypertension is evident but still hypothesized. This study aims to find the frequency of prehypertension and its association with blood group. Study Design: Cross sectional study. Setting:  Aziz Fatimah Medical and Dental College, Faisalabad. Period: 15 March’18 to 15 Sep’18. Material and Methods:  It was comprised of in comprised of 100 MBBS students. Demographic data including age, weight, height, waist and hip circumference, and BMI was determined. Blood group was determined with help of conventional slide method. Blood pressure was measured by auscultatory method. Data was analyzed by using SPSS 21. Continuous variables are given in mean and standard deviation. Categorical data was given in frequency and percentages. P value≤ 0.05 is taken as significant. Results: Out of 100 participants most common blood group was group ‘O’ (43%) followed by B (35 %) and AB (13%) and A (9%).36% of total population was pre-hypertensive and 64% were normotensive. Prehypertension is most commonly found in blood group ‘A’ (77%) followed by blood group ‘O’ (46.5%). Significant difference was found in means of SBP (p value= 0.001*) and DBP (p value= 0.000*) among the various blood groups. Conclusion: Most common blood group in studied population was ‘O’ although hypertension was most commonly observed in blood group A followed by ‘O’.

scholarly journals Distribution of ABO, Rhesus D and Subgroups of ABO among Blood Donors in Sokoto, North Western Nigeria

2020 ◽  
pp. 6-13
Author(s):  
A. B. Ibrahim ◽  
H. Attahiru ◽  
O. Erhabor ◽  
P. F. Udomah ◽  
A. Yakubu ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Groups ◽  
Blood Group A ◽  
Significant Difference ◽  
Tile Method ◽  
Group A ◽  
The Common ◽  
Group B ◽  
North Western

ABO, Rhesus D and subgroups of ABO are highly immunogenic and are the common cause of antibody production in mismatched blood transfusions, haemolytic transfusion reaction and maternal alloimmunization. The aim of this study was to determine the occurrence of ABO, Rh D and subgroups of ABO among blood donors attending Specialist Hospital Sokoto, Nigeria. ABO, Rhesus D and subgroups of ABO antigen status of 176 blood donors with mean age of 30.44 ± 8.210 years attending Specialist Hospital Sokoto were determined using tile method for ABO and Rh D and conventional tube method for anti- A1, anti- H reagents for ABO subgroups respectively. Among the 176 subjects tested, blood group O+ was the most frequent group with 93 (52.8%), 39 (22.2%) were blood group B+, 37(21.0%) were blood group A+, 5 (2.8%) were blood group AB+, 2 (1.1%) were blood group O-. No data was obtained for A-, B- and AB- blood groups.  Out of 37 A blood groups obtained, 31 (83.8%) had A1 antigens and 6 (16.2%) had A2 antigens. Out of the 5 AB blood groups, all had A1B antigens. The study also shows that there was statistically significant difference between blood group A and ethnic groups (Hausa, Fulani and Yoruba) (p<0.05). Blood group O was found to be the most frequent followed by B, A and AB except among Hausa which revealed a pattern of O> A> B> AB. ABO, subgroups shows majority had A1 followed by A2 and A1B respectively.

scholarly journals Factor VIII and Factor VIII Related Antigen in Normal Pregnancy

1977 ◽  
Author(s):  
G. O. S. de Melo
Keyword(s):  
Factor Viii ◽  
Pregnant Women ◽  
Blood Group ◽  
Normal Pregnancy ◽  
Clear Tendency ◽  
Factor Viii Related Antigen ◽  
Blood Group A ◽  
Significant Difference ◽  
Group A ◽  
In Pregnancy

Factor VIII and factor VIII related antigen were found to change proportionately during normal pregnancy. There was a clear tendency for levels of activity and antigen to increase as the duration of the pregnancy advanced. In the pregnant women studied a significant difference was observed in factor VIII levels between Blood Group A and Blood Group O. A similar difference was found in factor VIII related antigen levels. Age, weight and previous use of contraceptives seems to have no influence on factor VIII and factor VIII related antigen values in pregnancy.

Sign in / Sign up

Export Citation Format

Share Document