Effect of bicarbonate administration on skeletal muscle intracellular pH in the rat: implications for acute administration of bicarbonate in man

1992 ◽  
Vol 82 (5) ◽  
pp. 559-564 ◽  
Author(s):  
Campbell H. Thompson ◽  
Paul D. Syme ◽  
E. Mark Williams ◽  
John G. G. Ledingham ◽  
George K. Radda
Keyword(s):  
Carbon Dioxide ◽  
Skeletal Muscle ◽  
Partial Pressure ◽  
Intracellular Ph ◽  
Rate Of Change ◽  
Acute Administration ◽  
Intracellular Acidosis ◽  
Rat Skeletal Muscle ◽  
Bicarbonate Concentration

1. The effect of bicarbonate administration on the intracellular pH of rat skeletal muscle was examined by using 31P n.m.r. 2. Bicarbonate administered intraperitoneally caused a significant intracellular acidosis in rat skeletal muscle in vivo. When the bicarbonate was administered intravenously there was no such change in the pH of the skeletal muscle. 3. Bicarbonate administration by either route resulted in an elevated mixed venous partial pressure of carbon dioxide and an elevated arterial pH, but no significant change in the arterial partial pressure of carbon dioxide. The increase in arterial bicarbonate concentration after intraperitoneal injection of bicarbonate was delayed when compared with that after intravenous injection. 4. The administration of hypertonic solutions intravenously caused a transient 40–50% fall in blood pressure, which had resolved within 1 min. 5. The data suggest that the effect of bicarbonate administration on intracellular pH in vivo is related not only to carbon dioxide loading of the cell but also to the rate of change in the extracellular bicarbonate concentration.

Regulation of glucose transporter GLUT-4 and hexokinase II gene transcription by insulin and epinephrine

1997 ◽  
Vol 273 (4) ◽  
pp. E682-E687 ◽  
Author(s):  
Jared P. Jones ◽  
G. Lynis Dohm
Keyword(s):  
Skeletal Muscle ◽  
Gene Transcription ◽  
Glucose Transporter ◽  
Male Rats ◽  
Rat Skeletal Muscle ◽  
Hexokinase Ii ◽  
Epinephrine Infusion ◽  
Glut 4 ◽  
Gastrocnemius Muscles

Transport of glucose across the plasma membrane by GLUT-4 and subsequent phosphorylation of glucose by hexokinase II (HKII) constitute the first two steps of glucose utilization in skeletal muscle. This study was undertaken to determine whether epinephrine and/or insulin regulates in vivo GLUT-4 and HKII gene transcription in rat skeletal muscle. In the first experiment, adrenodemedullated male rats were fasted 24 h and killed in the control condition or after being infused for 1.5 h with epinephrine (30 μg/ml at 1.68 ml/h). In the second experiment, male rats were fasted 24 h and killed after being infused for 2.5 h at 1.68 ml/h with saline or glucose (625 mg/ml) or insulin (39.9 μg/ml) plus glucose (625 mg/ml). Nuclei were isolated from pooled quadriceps, tibialis anterior, and gastrocnemius muscles. Transcriptional run-on analysis indicated that epinephrine infusion decreased GLUT-4 and increased HKII transcription compared with fasted controls. Both glucose and insulin plus glucose infusion induced increases in GLUT-4 and HKII transcription of twofold and three- to fourfold, respectively, compared with saline-infused rats. In conclusion, epinephrine and insulin may regulate GLUT-4 and HKII genes at the level of transcription in rat skeletal muscle.

Trophic effect of iron-bound transferrin on acetylcholine receptors in rat skeletal muscle in vivo

1983 ◽  
Vol 38 (3) ◽  
pp. 303-307 ◽  
Author(s):  
Keiji Wada ◽  
Satoshi Ueno ◽  
Takanori Hazama ◽  
Hiro-O Yoshikawa ◽  
Saburo Ogasahara ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Acetylcholine Receptors ◽  
Rat Skeletal Muscle ◽  
Trophic Effect

Stable incorporation of a bacterial gene into adult rat skeletal muscle in vivo

1990 ◽  
Vol 258 (3) ◽  
pp. C578-C581 ◽  
Author(s):  
D. B. Thomason ◽  
F. W. Booth
Keyword(s):  
Skeletal Muscle ◽  
Leukemia Virus ◽  
Rat Skeletal Muscle ◽  
Bacterial Gene ◽  
Muscle Gene Expression ◽  
Adult Rat ◽  
Foreign Genes ◽  
Muscle Gene ◽  
Beta Galactosidase

We have developed a novel technique to incorporate and stably express foreign genes in adult rat skeletal muscle in vivo. Endogeneous satellite cells in skeletal muscle regenerating from bupivacaine damage were infected with an injected retrovirus containing the Escherichia coli beta-galactosidase gene under the promoter control of the Moloney murine leukemia virus long-terminal repeat. Constitutive and stable expression of beta-galactosidase activity was observed in muscle fibers after 6 days and 1 mo of muscle regeneration. Two patterns of expression were observed, diffuse expression within fibers and focal expression associated with the sarcolemma. This technique will allow future experiments with muscle-specific genes and promoters to study the physiological regulation of skeletal muscle gene expression in the intact adult mammal. Furthermore, the technique of stimulating stem cell proliferation to allow retroviral-mediated gene transfer may be generally applicable to other tissues.

scholarly journals Histological examinations of the in vivo biocompatibility of oxycellulose implanted into rat skeletal muscle

2018 ◽  
Vol 28 (5) ◽  
pp. 593-599
Author(s):  
Christiane Kunert-Keil ◽  
Isabel Narath ◽  
Jakub Hadzik ◽  
Tomasz Gedrange ◽  
Tomasz Gredes ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rat Skeletal Muscle

Endotoxemia causes a paradoxical intracellular pH recovery in exercising rat skeletal muscle

2007 ◽  
Vol 36 (4) ◽  
pp. 505-514 ◽  
Author(s):  
Benoît Giannesini ◽  
Marguerite Izquierdo ◽  
Christiane Dalmasso ◽  
Yann Le Fur ◽  
Patrick J. Cozzone ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Intracellular Ph ◽  
Rat Skeletal Muscle

N tau-methylhistidine release: contributions of rat skeletal muscle, GI tract, and skin

1982 ◽  
Vol 243 (4) ◽  
pp. E293-E297 ◽  
Author(s):  
S. J. Wassner ◽  
J. B. Li
Keyword(s):  
Skeletal Muscle ◽  
Gastrointestinal Tract ◽  
Soft Tissue ◽  
Fractional Catabolic Rate ◽  
Gi Tract ◽  
Rat Skeletal Muscle ◽  
Catabolic Rate ◽  
Total Body

The relative contributions of skeletal muscle, gastrointestinal tract, and skin to urinary N tau-methylhistidine (MH) excretion were estimated during in vitro studies using the rat hemicorpus preparation. After 0.5 h of perfusion, MH release into the perfusate was linear for 3 h and averaged 29.8 nmol . h-1 . 100 g hemicorpus-1. In vivo, 24-h urinary MH excretion averaged 37.3 nmol . h-1 . 100 g body wt-1. The ratio of soft tissue to skin weight is equal (3.2:1) in the whole rat and in the hemicorpus. The gastrointestinal tract released 16.0 nmol . h-1 . 100 g body wt-1 or approximately 41% of the total urinary MH excretion. Preparations perfused with or without skin showed modest differences in the rate of MH release that were not statistically significant. Skeletal muscle contains 89.8% of total body MH content, whereas gastrointestinal tract and skin contain 3.8 and 6.4%, respectively. Gastrointestinal tract actomyosin turns over rapidly with a fractional catabolic rate of 24%/day versus 1.4%/day for skeletal muscle actomyosin.

Ischemia/reperfusion-induced changes in intracellular free Ca2+ levels in rat skeletal muscle fibers – an in vivo study

2000 ◽  
Vol 440 (2) ◽  
pp. 302-308 ◽  
Author(s):  
Tamás Ivanics ◽  
Zsuzsa Miklós ◽  
Zoltán Ruttner ◽  
Sándor Bátkai ◽  
Dick W. Slaaf ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Ischemia Reperfusion ◽  
Muscle Fibers ◽  
In Vivo Study ◽  
Rat Skeletal Muscle ◽  
Skeletal Muscle Fibers ◽  
Induced Changes

pH of isolated resting skeletal muscle and its relation to potassium content

1963 ◽  
Vol 204 (6) ◽  
pp. 1048-1054 ◽  
Author(s):  
Ronald B. Miller ◽  
Ian Tyson ◽  
Arnold S. Relman
Keyword(s):  
Skeletal Muscle ◽  
Intracellular Ph ◽  
Potassium Content ◽  
Ion Concentration ◽  
Detectable Effect ◽  
Rat Diaphragm ◽  
Intracellular Acidosis ◽  
Experimental Conditions ◽  
Hydrogen Ion Concentration

Intracellular pH of isolated rat diaphragm was measured with both a C14-DMO method and a tissue CO2 technique. The values for intracellular pH by each method, although slightly different, changed in parallel under most experimental conditions. Acute, severe potassium depletion in vitro had no detectable effect on intracellular pH, nor did prior depletion in vivo followed by incubation in a potassium-free bath. This was true whether or not the potassium-depleted muscle was exposed to normal or elevated extracellular levels of bicarbonate, and was unaffected by the presence of cationic amino acids in the bath. Acute repletion of previously potassium-depleted muscle resulted in a small rise in cell pH, but this was no greater than that produced by loading normal tissues with potassium. It is concluded that under the conditions of these experiments there is no evidence of intracellular acidosis in potassium-depleted skeletal muscle. Rat diaphragm can lose up to half its potassium content in vitro without detectable increase in hydrogen ion concentration.

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