Cholyl-Lysyl-Fluorescein Isothiocyanate: Biliary Kinetics in Vivo and during Single-Pass Perfusion of Isolated Perfused Rat Liver

1988 ◽  
Vol 75 (s19) ◽  
pp. 9P-9P ◽  
Author(s):  
CO Mills ◽  
K Rahman ◽  
R Coleman ◽  
E Elias
Keyword(s):  
Rat Liver ◽  
Fluorescein Isothiocyanate ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Single Pass ◽  
Pass Perfusion ◽  
Kinetics In Vivo ◽  
Biliary Kinetics

scholarly journals Metabolism of inorganic sulphate in the isolated perfused rat liver. Effect of sulphate concentration on the rate of sulphation by phenol sulphotransferase

1978 ◽  
Vol 176 (3) ◽  
pp. 959-965 ◽  
Author(s):  
Gerard J. Mulder ◽  
Katja Keulemans
Keyword(s):  
Steady State ◽  
Plasma Concentration ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Perfusion Medium ◽  
Physiological Concentration ◽  
Sulphate Concentration ◽  
Inorganic Sulphate

1. The metabolism of inorganic [35S]sulphate (Na235SO4) was studied in the isolated perfused rat liver at three initial concentrations of inorganic sulphate in the perfusion medium (0, 0.65 and 1.30mm), in relation to sulphation and glucuronidation of a phenolic drug, harmol (7-hydroxy-1-methyl-9H-pyrido[3,4-b]indole). 2. [35S]Sulphate rapidly equilibrated with endogenous sulphate in the liver. It was excreted in bile and reached, at the lowest concentration in the perfusion medium, concentrations in bile that were much higher than those in the perfusion medium; at the higher sulphate concentrations, these concentrations were equal. The physiological concentration of inorganic sulphate in the liver, available for sulphation of drugs, is similar to the plasma concentration. 3. At zero initial inorganic sulphate in the perfusion medium, the rate of sulphation was very low and harmol was mainly glucuronidated. At 0.65mm-sulphate glucuronidation was much decreased and considerable sulphation took place, indicating efficient competition of conjugation by sulphation. At 1.30mm-sulphate the sulphation increased still further. 4. The results suggest that an important factor in sulphation is the relatively high Km of synthesis of adenosine 3′-phosphate 5′-sulphatophosphate (the co-substrate of sulphation) for inorganic sulphate, which is of the order of the plasma concentration of inorganic sulphate. The steady-state adenosine 3′-phosphate 5′-sulphatophosphate concentration may determine the rate of sulphate conjugation of drugs in the rat in vivo.

Bioassay of endotoxin clearance in vivo and by perfused rat liver

1976 ◽  
Vol 231 (1) ◽  
pp. 258-264 ◽  
Author(s):  
BJ Buchanan ◽  
JP Filkins
Keyword(s):  
Linear Relationship ◽  
Rat Liver ◽  
Salt Solution ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Half Time ◽  
Rat Blood ◽  
Endotoxin Concentration ◽  
Induction Of Tolerance

Endotoxin clearances in vivo and by the isolated perfused rat liver were evaluated via bioassay in lead-sensitized rats. A linear relationship between the probit of shock lethality and the endotoxin dose in the probit range of 4-6 was validated. Endotoxin clearance in normal, fed rats displayed a linear relationship between the logarithm of the blood endotoxin concentration and time throughout the period of 15-240 min at doses of 500 and 1,000 mug/ rat; the half-time values were 58-63 min. Decreasing the endotoxin dose to 250 mug resulted in multiphasic clearance curves. Induction of tolerance to endotoxin resulted in marked acceleration of endotoxin clearance. Endotoxin clearance from the isolated perfused rat liver was not influenced by serum or rat blood as compared to clearance from a balanced salt solution. These data suggest that a physiologically stressful dose of endotoxin is slowly cleared from the blood and, therefore, circulates for prolonged periods.

Metabolic characteristics of the isolated perfused rat liver

1960 ◽  
Vol 199 (3) ◽  
pp. 395-399 ◽  
Author(s):  
Alvin S. Ostashever ◽  
Irving Gray ◽  
Samuel Graff
Keyword(s):  
Rat Liver ◽  
Amino Nitrogen ◽  
Experimental Period ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Metabolic Characteristics ◽  
Respiration Rates ◽  
Substrate Respiration ◽  
Body Production

The use of the isolated perfused rat liver for quantitative biochemical studies of liver metabolism over an experimental period of as long as 4 hours is demonstrated. Respiration rates responded immediately to the addition of substrates and the liver could be restimulated by a second dose of substrate, respiration rates reflecting the nature of the substrate. Net amino nitrogen uptake was consistently exceeded by urea nitrogen production, the latter comparing favorably with in vivo rates. Fructose was rapidly removed from the perfusate by the liver, and the liver efficiently removed lactic acid formed by erythrocyte glycolysis. Ketone body production was continuous and within normal in vivo rates. Bile production declined gradually over a 4-hour period.

scholarly journals Metabolism of ornithine, α-ketoglutarate and arginine in isolated perfused rat liver

1995 ◽  
Vol 73 (2) ◽  
pp. 227-239 ◽  
Author(s):  
Jean Pascal De Bandt ◽  
Luc Cynober ◽  
Soo Kyung Lim ◽  
Colette Coudray-Lucas ◽  
Raoul Poupon ◽  
...  
Keyword(s):  
Rat Liver ◽  
Hepatic Metabolism ◽  
Isolated Perfused Rat Liver ◽  
Trauma Patients ◽  
Perfused Rat Liver ◽  
Metabolic Interaction ◽  
Urea Production ◽  
Stimulation Of

Ornithine (Orn; α-ketoglutarate (αKG) salt) and arginine (Arg) supplementation of enteral diets has been advocated in the treatment of hypercatabolism of trauma patients, but both compounds are subject to extensive hepatic metabolism. To compare the metabolism of these two compounds and to evaluate the possible influence of the αKG moiety, livers were perfused with αKG, Orn, ornithine α-ketoglutarate (OKG) or Arg (n 6 in each group) for 1 h. Arg uptake was nearly fourfold higher than Orn uptake (690 (SD 162) ν. 178 (SD 30) nmol/min per g liver), and Orn uptake was not modified by αKG. Orn was totally metabolized by the liver, whereas Arg led to Orn release (408 (SD 159) nmol/min per g liver) and a threefold stimulation of urea production (Arg 1·44 (SD 0·22) ν. Orn 0·45 (SD 0.09) μol/min per g liver). αKG alone only increased hepatic aspartate uptake but, when associated with Orn as OKG, it led to an increase in giutamate release and in proiine content in the liver and to a decrease in proiine uptake. From these findings we conclude that (1) Arg load is extensively metabolized by the liver, inducing urea production, (2) in enteral use, Orn supplementation appears preferable to Arg as it is less ureogenic (as also recently demonstrated in vivo in stressed rats receiving isomolar amounts of Arg and Orn), (3) the liver participates in the Orn-αKG metabolic interaction, mostly in proiine metabolism, which occurs in the splanchnic area.

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