Effect of changes in dietary sodium on active electrolyte transport by erythrocytes at different stages of human pregnancy

1988 ◽  
Vol 74 (2) ◽  
pp. 145-150 ◽  
Author(s):  
E. D. M. Gallery ◽  
J. Rowe ◽  
M. A. Brown ◽  
M. Ross
Keyword(s):  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Normal Values ◽  
Sodium Intake ◽  
Post Partum ◽  
Electrolyte Transport ◽  
Dietary Sodium ◽  
Ouabain Binding ◽  
86Rb Influx ◽  
In Pregnancy

1. Active electrolyte transport was examined in erythrocytes from women in the second and third trimesters of pregnancy and post partum, and compared with that in ovulating women. 2. There was a significant reduction in intracellular sodium ([Na]i) and increase in intracellular potassium ([K]i) in pregnancy with a return towards normal values in the post-partum period. 3. Maximum specific ouabain binding [number of Na+,K+-adenosine triphosphatase (Na+,K+-ATPase) units] was increased by 70% in pregnancy and returned slowly towards normal values post partum. 4. Na+,K+-ATPase activity as determined by ouabain-sensitive 86Rb influx in artificial media was also increased in pregnancy by 13%. It returned towards normal post partum. 5. The increases in Na+,K+-ATPase in pregnancy were not closely related to the concomitant increases in aldosterone or cholesterol nor to reticulocytosis and were not affected by 7 days of high (> 250 mmol/day) or low (< 50 mmol/day) sodium intake.

scholarly journals Ouabain binding to phospholipid-dependent adenosine triphosphatase

1978 ◽  
Vol 169 (2) ◽  
pp. 313-320 ◽  
Author(s):  
S L Goodman ◽  
K P Wheeler
Keyword(s):  
Atpase Activity ◽  
Protein Complex ◽  
Adenosine Triphosphatase ◽  
Rabbit Kidney ◽  
Ouabain Binding ◽  
Equilibrium Conditions ◽  
Enzyme Preparations

The role of phospholipid in the binding of ouabain to the (Na+ + K+)-dependent adenosine triphosphatase was studied. Enzyme preparations obtained from rabbit kidney were treated with Lubrol WX to remove the phospholipid component essential for ATPase activity. Reconstituted enzyme samples were prepared by the addition of phosphatidylserine and sedimentation of an enzymically active lipid-protein complex. The binding of ouabain to both kinds of preparations was measured under equilibrium conditions with the use of 3H-labelled ouabain and initial ouabain concentrations in the range 0.01-1 micrometer. The main findings were: (i) (Mg2+ + Pi) promoted binding of significant quantities of ouabain only to the reconstituted enzyme; (ii) the absence of added Na+, (Mg2+ + ATP) similarly promoted binding only to the reconstituted samples; (iii) the addition of Na+ in the presence of (Mg2+ + ATP) increased the amount of ouabain bound to the reconstituted enzyme when the ouabain concentration was below about 0.1 micrometer, but it had no effect when the ouabain concentration was about 1 micrometer; (iv) (Mg2+ + ATP) induced ouabain binding to the depleted enzyme only when Na+ was also added; (v) the amount of ouabain bound to both depleted and reconstituted enzymes was the same in the presence of (Mg2+ + ATP + Na+); (vi) the reconstituted enzyme appeared to have a greater affinity for Na+ than did the depleted enzyme.

Comparing methods to assess dietary sodium intake in pregnancy

1987 ◽  
Vol 87 (8) ◽  
pp. 1058-1060
Author(s):  
Mark A. Brown ◽  
Joanne S. Prendergast ◽  
Margaret R. Ross ◽  
Eileen D.M. Gallery
Keyword(s):  
Sodium Intake ◽  
Dietary Sodium ◽  
In Pregnancy

scholarly journals Effects of diuretic treatment and of dietary sodium on renal binding of 3H-metolazone.

1990 ◽  
Vol 1 (1) ◽  
pp. 91-98
Author(s):  
Z F Chen ◽  
D A Vaughn ◽  
K Beaumont ◽  
D D Fanestil
Keyword(s):  
Atpase Activity ◽  
Chronic Conditions ◽  
Cellular Structure ◽  
Sodium Intake ◽  
Normal Growth ◽  
Dietary Sodium ◽  
Sodium Reabsorption ◽  
Distal Nephron ◽  
Experimental Conditions ◽  
Series Of Experiments

We report a series of experiments designed to determine if agents and conditions that have been reported to alter sodium reabsorption, Na-K-ATPase activity or cellular structure in the rat distal nephron might also regulate the density or affinity of binding of 3H-metolazone to the putative thiazide receptor in the distal nephron. Experimental conditions selected for study were acute (60-min) and chronic hydrochlorothiazide (HCTZ), acute acetazolamide, acute and chronic furosemide, and 14 days of varied intake of dietary sodium. The density of the binding of 3H-metolazone was increased 47% by acute HCTZ (P less than 0.001) and 39% (P less than 0.001) by acute furosemide. In contrast, acute acetazolamide produced no change in binding despite eliciting a dramatic diuresis. Chronic HCTZ (5 days) and chronic furosemide (7 days) increased binding of 3H-metolazone by 46% (P less than 0.001) and by 101% (P less than 0.001), respectively. Variation of dietary sodium intake over a range that allowed normal growth of the animal and that produced urinary excretion of Na varying from 0.28 to 2.62 mEq/100 g/day failed to alter the density of binding of 3H-metolazone. These studies are the first indication that the density of the thiazide receptor is regulated by a variety of both acute and chronic conditions that have previously been associated with changes in transport, ultrastructure or Na-K-ATPase activity in the distal nephron.(ABSTRACT TRUNCATED AT 250 WORDS)

Localization of Adenosine Triphosphatase Activity in the Phleom of Nicotiana Tabacum

1972 ◽  
Vol 30 ◽  
pp. 230-231
Author(s):  
James Cronshaw ◽  
Jamison E. Gilder
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Higher Plants ◽  
High Surface ◽  
Root Tip ◽  
Animal Cells ◽  
Physiological Processes ◽  
Tip Cells ◽  
Atpase Localization

Adenosine triphosphatase (ATPase) activity has been shown to be associated with numerous physiological processes in both plants and animal cells. Biochemical studies have shown that in higher plants ATPase activity is high in cell wall preparations and is associated with the plasma membrane, nuclei, mitochondria, chloroplasts and lysosomes. However, there have been only a few ATPase localization studies of higher plants at the electron microscope level. Poux (1967) demonstrated ATPase activity associated with most cellular organelles in the protoderm cells of Cucumis roots. Hall (1971) has demonstrated ATPase activity in root tip cells of Zea mays. There was high surface activity largely associated with the plasma membrane and plasmodesmata. ATPase activity was also demonstrated in mitochondria, dictyosomes, endoplasmic reticulum and plastids.

Low back pain in pregnancy and post-partum period. Principles for medical rehabilitation

2018 ◽  
Vol 1 (19) ◽  
pp. 22
Author(s):  
Iulia Filipescu ◽  
Mihai Berteanu ◽  
George Alexandru Filipescu ◽  
Radu Vlădăreanu
Keyword(s):  
Low Back Pain ◽  
Back Pain ◽  
Post Partum ◽  
Medical Rehabilitation ◽  
Low Back ◽  
Post Partum Period ◽  
In Pregnancy

ß-ENDORPHIN RESPONSES TO VARIATION OF DIETARY SODIUM INTAKE

Analgesia ◽  
1995 ◽  
Vol 1 (4) ◽  
pp. 520-523
Author(s):  
Karin Kraft
Keyword(s):  
Sodium Intake ◽  
Dietary Sodium
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