The Activity of the Branched-Chain 2-Oxo Acid Dehydrogenase in Human Muscle before and after Exercise

1987 ◽  
Vol 73 (s17) ◽  
pp. 53P-53P
Author(s):  
AJM Wagenmakers ◽  
JH Brookes ◽  
JH Coakley ◽  
T Reilly ◽  
RHT Edwards
Keyword(s):  
Human Muscle ◽  
Acid Dehydrogenase ◽  
Before And After ◽  
Branched Chain

Exercise-induced activation of the branched-chain 2-oxo acid dehydrogenase in human muscle

1989 ◽  
Vol 59 (3) ◽  
pp. 159-167 ◽  
Author(s):  
Anton J. M. Wagenmakers ◽  
John H. Brookes ◽  
John H. Coakley ◽  
Thomas Reilly ◽  
Richard H. T. Edwards
Keyword(s):  
Human Muscle ◽  
Acid Dehydrogenase ◽  
Branched Chain ◽  
Exercise Induced

scholarly journals The activity state of the branched-chain 2-oxo acid dehydrogenase complex in rat tissues

1984 ◽  
Vol 220 (1) ◽  
pp. 273-281 ◽  
Author(s):  
A J M Wagenmakers ◽  
J T G Schepens ◽  
J A M Veldhuizen ◽  
J H Veerkamp
Keyword(s):  
Skeletal Muscle ◽  
High Sensitivity ◽  
Rat Tissues ◽  
Fed State ◽  
Acid Dehydrogenase ◽  
Before And After ◽  
Liver And Kidney ◽  
Branched Chain ◽  
Acid Dehydrogenase Complex ◽  
Dehydrogenase Complex

An assay is described to define the proportion of the branched-chain 2-oxo acid dehydrogenase complex that is present in the active state in rat tissues. Activities are measured in homogenates in two ways: actual activities, present in tissues, by blocking both the kinase and phosphatase of the enzyme complex during homogenization, preincubation, and incubation with 1-14C-labelled branched-chain 2-oxo acid, and total activities by blocking only the kinase during the 5 min preincubation (necessary for activation). The kinase is blocked by 5 mM-ADP and absence of Mg2+ and the phosphatase by the simultaneous presence of 50 mM-NaF. About 6% of the enzyme is active in skeletal muscle of fed rats, 7% in heart, 20% in diaphragm, 47% in kidney, 60% in brain and 98% in liver. An entirely different assay, which measures activities in crude tissue extracts before and after treatment with a broad-specificity protein phosphatase, gave similar results for heart, liver and kidney. Advantages of our assay with homogenates are the presence of intact mitochondria, the simplicity, the short duration and the high sensitivity. The actual activities measured indicate that the degradation of branched-chain 2-oxo acids predominantly occurs in liver and kidney and is limited in skeletal muscle in the fed state.

EFFECT OF CARBOHYDRATE AVAILABILITY ON EXERCISE-INDUCED ACTIVATION OF THE BRANCHED-CHAIN 2-OXO ACID DEHYDROGENASE IN HUMAN MUSCLE

1980 ◽  
Vol 21 (Supplement) ◽  
pp. S106
Author(s):  
A. J.M. Wagenmakers ◽  
E. Beckers ◽  
F. Broung ◽  
H. Kuipers ◽  
G. van de Vusse ◽  
...  
Keyword(s):  
Human Muscle ◽  
Carbohydrate Availability ◽  
Acid Dehydrogenase ◽  
Branched Chain ◽  
Exercise Induced

EFFECT OF CARBOHYDRATE AVAILABILITY ON EXERCISE-INDUCED ACTIVATION OF THE BRANCHED-CHAIN 2-OXO ACID DEHYDROGENASE IN HUMAN MUSCLE

1989 ◽  
Vol 21 (Supplement) ◽  
pp. S106 ◽  
Author(s):  
A. J.M. Wagenmakers ◽  
E. Beckers ◽  
F. Broung ◽  
H. Kuipers ◽  
G. van de Vusse ◽  
...  
Keyword(s):  
Human Muscle ◽  
Carbohydrate Availability ◽  
Acid Dehydrogenase ◽  
Branched Chain ◽  
Exercise Induced
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