Erythrocyte aldehyde dehydrogenase activity in alcoholic subjects and its value as a marker for hepatic aldehyde dehydrogenase in subjects with and without liver disease

1986 ◽  
Vol 70 (3) ◽  
pp. 295-299 ◽  
Author(s):  
K. Matthewson ◽  
C. O. Record
Keyword(s):  
Liver Disease ◽  
Dehydrogenase Activity ◽  
Aldehyde Dehydrogenase ◽  
Alcoholic Liver Disease ◽  
Study Groups ◽  
Liver Disorder ◽  
Control Group ◽  
Liver Disorders ◽  
Normal Controls ◽  
Aldehyde Dehydrogenase Activity

1. Erythrocyte aldehyde dehydrogenase activity was assayed in actively drinking alcoholics, patients with alcoholic liver disease who claimed to be abstaining, patients with non-alcoholic liver disorders and normal controls. Hepatic cytosolic aldehyde dehydrogenase was also assayed in the majority of the subjects. 2. Actively drinking alcoholics had significantly lower erythrocyte aldehyde dehydrogenase activity than controls (P < 0.01) but abstaining alcoholic liver disease and non-alcoholic liver disorder subjects did not. 3. There was a significant correlation between erythrocyte and hepatic cytosolic aldehyde dehydrogenase activity in the control group (r = 0.94, P < 0.05) but not in the other study groups.

Alcohol Dehydrogenase Isoenzymes and Aldehyde Dehydrogenase Activity in the Serum of Patients with Non-alcoholic Fatty Liver Disease

2018 ◽  
Vol 38 (7) ◽  
pp. 4005-4009 ◽  
Author(s):  
WOJCIECH JELSKI ◽  
BLANKA WOLSZCZAK-BIEDRZYCKA ◽  
ELŻBIETA ZASIMOWICZ-MAJEWSKA ◽  
KAROLINA ORYWAL ◽  
TADEUSZ WOJCIECH LAPINSKI ◽  
...  
Keyword(s):  
Liver Disease ◽  
Alcohol Dehydrogenase ◽  
Fatty Liver ◽  
Dehydrogenase Activity ◽  
Aldehyde Dehydrogenase ◽  
Fatty Liver Disease ◽  
Alcohol Dehydrogenase Isoenzymes ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease ◽  
Aldehyde Dehydrogenase Activity

Influence of chronic alcohol abuse and liver disease on hepatic aldehyde dehydrogenase activity

Alcohol ◽  
1998 ◽  
Vol 15 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Francesc Vidal ◽  
Roca Toda ◽  
Cristina Gutiérrez ◽  
Montserrat Broch ◽  
Fina Fernández-Muixí ◽  
...  
Keyword(s):  
Liver Disease ◽  
Alcohol Abuse ◽  
Dehydrogenase Activity ◽  
Aldehyde Dehydrogenase ◽  
Chronic Alcohol ◽  
Chronic Alcohol Abuse ◽  
Aldehyde Dehydrogenase Activity

Effect of liver disorders on ethanol elimination and alcohol and aldehyde dehydrogenase activities in liver and erythrocytes

1989 ◽  
Vol 76 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Antonio Zorzano ◽  
Luis Ruiz del Arbol ◽  
Emilio Herrera
Keyword(s):  
Liver Disease ◽  
Aldehyde Dehydrogenase ◽  
Alcoholic Liver Disease ◽  
Ethanol Metabolism ◽  
Control Group ◽  
Aldh Activity ◽  
Healthy Control ◽  
Ethanol Elimination ◽  
Kinetics Of ◽  
Adh Activity

1. Liver biopsies were performed in healthy control subjects and in subjects with alcoholic and non-alcoholic liver disease in order to examine alcohol dehydrogenase (ADH; EC 1.1.1.1) and aldehyde dehydrogenase [ALDH; aldehyde dehydrogenase (NAD+); EC 1. 2. 1. 3] activities. Erythrocyte ALDH and ethanol metabolism were also investigated in the same subjects. 2. Fifteen per cent of the subjects studied (seven of 48 subjects tested) presented atypical ADH activity, characterized by elevated activity at pH 7.4 or 8.8 compared with that found in subjects with the usual ADH form. However, the ethanol elimination curves obtained in two subjects with atypical ADH were indistinguishable from the kinetics of the group with normal ADH. Subjects displaying atypical ADH activity showed normal liver and erythrocyte ALDH activities. 3. Considering only the subjects with the normal ADH form, hepatic ADH activity was unaltered in subjects with non-alcoholic liver disease (chronic hepatitis or cirrhosis) and in those with alcoholic steatosis. Subjects with alcoholic hepatitis or alcoholic cirrhosis showed a lower ADH activity compared with the healthy control group. 4. In spite of the changes detected in subjects with alcoholic liver disease, curves of blood ethanol concentration after oral administration of 0.4 g of ethanol/kg were indistinguishable between the alcoholic hepatitis group and the control group. 5. Hepatic ALDH activity, assayed at 300 μmol/l acetaldehyde, was found to be diminished in all liver pathologies investigated, regardless of their aetiology. Nevertheless, erythrocyte ALDH activity was not modified in subjects with non-alcoholic or alcoholic liver disease. As a result of these findings, no relationship was found between hepatic and erythrocyte ALDH. 6. In summary, our data demonstrate that (a) marked modifications in ADH activity, as found in patients with atypical ADH or in subjects with alcoholic liver disease, are not accompanied by parallel alterations in the kinetics of ethanol disappearance, suggesting that ADH activity per se does not limit ethanol metabolism in vivo, (b) hepatic high-Km ALDH activity is decreased in patients with liver disease independent of alcoholism, and therefore decreased ALDH activity cannot be considered as a primary defect in alcoholism but as a consequence of liver damage, and (c) erythrocyte ALDH does not reflect hepatic high-Km ALDH.

Metabolism of vitamin D in patients with primary biliary cirrhosis and alcoholic liver disease

1985 ◽  
Vol 69 (5) ◽  
pp. 561-570 ◽  
Author(s):  
E. Barbara Mawer ◽  
H. J. Klass ◽  
T. W. Warnes ◽  
Jacqueline L. Berry
Keyword(s):  
Vitamin D ◽  
Liver Disease ◽  
Primary Biliary Cirrhosis ◽  
Alcoholic Liver Disease ◽  
Vitamin D3 ◽  
Control Group ◽  
Biliary Cirrhosis ◽  
Vitamin D2 ◽  
Dihydroxyvitamin D3 ◽  
Poor Renal Function

1. The metabolism of isotopically labelled vitamin D2 and D3 has been investigated in eight patients with primary biliary cirrhosis and in five controls. The concentration of labelled vitamin D2 was lower than that of vitamin D3 in serum of patients with primary biliary cirrhosis on days 1 and 2 after intravenous injection (P < 0.005 and P < 0.05, respectively) but no difference was seen in controls. 2. Similar amounts of labelled 25-hydroxyvitamin D2 and D3 were seen in serum of the control group; the same pattern was observed in the primary biliary cirrhosis group, and no significant differences were observed between the two groups. 3. In both control and primary biliary cirrhosis groups, the serum concentration of labelled 24,25-dihydroxyvitamin D2 exceeded that of 24,25-dihydroxyvitamin D3 (significant for controls on day 2, P < 0.02) but concentrations in the two groups were not different. 4. Concentrations of labelled 25,26-dihydroxyvitamin D3 were significantly higher than those of 25,26-dihydroxyvitamin D2 in the primary biliary cirrhosis group at all times and in the control group on days 2 and 3. Both 25,26-dihydroxyvitamin D2 and D3 were higher in the serum of patients with primary biliary cirrhosis than in controls (significant on day 1, P < 0.05). 5. Urinary excretion over days 0–3 of radioactivity from both vitamins D2 and D3 was significantly higher in the primary biliary cirrhosis group than in controls: 12.03 vs 1.80% for vitamin D2 and 8.98 vs 1.76% for vitamin D3(P < 0.005). Vitamin D2-derived urinary radioactivity in primary biliary cirrhosis correlated strongly with serum bilirubin (P = 0.005). 6. The metabolism of labelled vitamin D3 was studied in seven patients with alcoholic liver disease, three of whom showed low serum concentrations of labelled 25-hydroxyvitamin D3 suggesting impaired hepatic synthesis. The 25-hydroxylation response was quantified as the relative index of 25-hydroxylation and was significantly related to two other indices of liver function. It is concluded that impaired 25-hydroxylation of vitamin D may occur in alcoholic liver disease and results from hepatocellular dysfunction. 7. Less than the predicted amounts of 1,25-dihydroxyvitamin D3 were produced in four of the seven patients with alcoholic liver disease; this defect may be attributable in part to decreased precursor 25-hydroxyvitamin D and to poor renal function.

Diminished alcohol preference in transgenic mice lacking aldehyde dehydrogenase activity

2002 ◽  
Vol 12 (8) ◽  
pp. 621-626 ◽  
Author(s):  
Toyohi Isse ◽  
Tsunehiro Oyama ◽  
Kyoko Kitagawa ◽  
Koji Matsuno ◽  
Akiko Matsumoto ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Dehydrogenase Activity ◽  
Aldehyde Dehydrogenase ◽  
Alcohol Preference ◽  
Aldehyde Dehydrogenase Activity
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