Biochemical and Histochemical Demonstration of Transferrin Receptors on Parenchymal and Non-Parenchymal Cells of Rat Liver

1985 ◽  
Vol 68 (s11) ◽  
pp. 63P-63P
Author(s):  
W Vogel ◽  
SP Young ◽  
A Bomford ◽  
Roger Williams
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration ◽  
Transferrin Receptors ◽  
Parenchymal Cells

scholarly journals Liver endothelium and not hepatocytes or Kupffer cells have transferrin receptors

Blood ◽  
1984 ◽  
Vol 63 (2) ◽  
pp. 270-276
Author(s):  
R Soda ◽  
M Tavassoli
Keyword(s):  
Endothelial Cells ◽  
Rat Liver ◽  
Kupffer Cells ◽  
Liver Cell ◽  
Cell Suspensions ◽  
Transferrin Receptors ◽  
Visual Probe ◽  
Covalently Linked ◽  
Parenchymal Cells

Using a visual probe, consisting of latex minibeads covalently linked to transferrin (TF), we found that, in rat liver cell suspensions, transferrin receptors were limited to endothelial cells. Neither hepatocytes nor Kupffer cells contained an appreciable number of TF receptors. Specificity of this reaction was demonstrated by preincubation with non-derivatized TF, which inhibited the binding. This was further confirmed by fractionation of liver cell suspensions on metrizamide gradients. The uptake of either the visual probe or 125I- labeled TF was again limited to the endothelium-rich fraction. Transferrin bound to endothelial membrane was internalized at 37 degrees C, but not at 4 degrees C, via a coated pit system. Again, hepatocytes and Kupffer cells did not internalize the probe. The findings suggest that iron may be first taken up by liver endothelium and then transmitted to parenchymal cells. These results emphasize the generally unappreciated role of endothelium in the transport across the tissue-blood barrier.

scholarly journals Liver endothelium and not hepatocytes or Kupffer cells have transferrin receptors

Blood ◽  
1984 ◽  
Vol 63 (2) ◽  
pp. 270-276 ◽  
Author(s):  
R Soda ◽  
M Tavassoli
Keyword(s):  
Endothelial Cells ◽  
Rat Liver ◽  
Kupffer Cells ◽  
Liver Cell ◽  
Cell Suspensions ◽  
Transferrin Receptors ◽  
Visual Probe ◽  
Covalently Linked ◽  
Parenchymal Cells

Abstract Using a visual probe, consisting of latex minibeads covalently linked to transferrin (TF), we found that, in rat liver cell suspensions, transferrin receptors were limited to endothelial cells. Neither hepatocytes nor Kupffer cells contained an appreciable number of TF receptors. Specificity of this reaction was demonstrated by preincubation with non-derivatized TF, which inhibited the binding. This was further confirmed by fractionation of liver cell suspensions on metrizamide gradients. The uptake of either the visual probe or 125I- labeled TF was again limited to the endothelium-rich fraction. Transferrin bound to endothelial membrane was internalized at 37 degrees C, but not at 4 degrees C, via a coated pit system. Again, hepatocytes and Kupffer cells did not internalize the probe. The findings suggest that iron may be first taken up by liver endothelium and then transmitted to parenchymal cells. These results emphasize the generally unappreciated role of endothelium in the transport across the tissue-blood barrier.

scholarly journals Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.

1976 ◽  
Vol 251 (10) ◽  
pp. 3014-3020 ◽  
Author(s):  
R F Kletzien ◽  
M W Pariza ◽  
J E Becker ◽  
V R Potter ◽  
F R Butcher
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Amino Acid Transport ◽  
Primary Cultures ◽  
Acid Transport ◽  
Liver Parenchymal ◽  
Adult Rat ◽  
Parenchymal Cells
Sign in / Sign up

Export Citation Format

Share Document