Therapeutic Aspects of Branched-Chain Amino and Keto Acids

1984 ◽  
Vol 66 (1) ◽  
pp. 1-15 ◽  
Author(s):  
MacKenzie Walser
Keyword(s):  
Keto Acids ◽  
Branched Chain

Influence of branched-chain amino acids and branched-chain keto acids on protein synthesis in isolated hepatocytes

Hepatology ◽  
1987 ◽  
Vol 7 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Wolfgang Base ◽  
Carl Barsigian ◽  
Alisa Schaeffer ◽  
Ellen Shaw ◽  
Jose Martinez ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Isolated Hepatocytes ◽  
Branched Chain Amino Acids ◽  
Keto Acids ◽  
Branched Chain ◽  
Branched Chain Keto Acids

Transamination of branched-chain keto acids by isolated perfused rat kidney.

1978 ◽  
Vol 235 (1) ◽  
pp. E47
Author(s):  
W E Mitch ◽  
W Chan
Keyword(s):  
Amino Acids ◽  
Rat Kidney ◽  
Branched Chain Amino Acids ◽  
Keto Acid ◽  
Branched Chain Amino Acid ◽  
Keto Acids ◽  
Perfused Rat Kidney ◽  
Branched Chain ◽  
Branched Chain Keto Acids ◽  
Isolated Rat

Isolated rat kidney perfused without substrate released serine, glycine, and taurine, and substantially smaller amounts of other amino acids. When branched-chain keto acids were added, the corresponding amino acids were released at rates amounting to 15-25% of keto acid disappearance. Perfusion with 2 mM alpha-keto-isovalerate or alpha-keto-beta-methylvalerate caused an increased glucose release amounting to 18-23% of keto acid disappearance. The activity of branched-chain amino acid transferase (BATase) was significantly stimulated by perfusion with the analogue of leucine, but not by perfusion with alpha-ketoglutarate, the analogues of valine or isoleucine, or with leucine itself. These findings document that the kidney converts branched-chain keto acids in part to the corresponding amino acids and suggest that the keto analogue of leucine may be involved in the control of renal BATase activity, thereby indirectly regulating the metabolism of branched-chain amino acids.

scholarly journals CodY-Regulated Aminotransferases AraT and BcaT Play a Major Role in the Growth of Lactococcus lactis in Milk by Regulating the Intracellular Pool of Amino Acids

2003 ◽  
Vol 69 (6) ◽  
pp. 3061-3068 ◽  
Author(s):  
Emilie Chambellon ◽  
Mireille Yvon
Keyword(s):  
Amino Acids ◽  
Growth Inhibition ◽  
Lactococcus Lactis ◽  
Double Mutant ◽  
Wild Type Strain ◽  
Aromatic Amino Acids ◽  
Nitrogen Sources ◽  
Nutritional Factors ◽  
Keto Acids ◽  
Branched Chain

ABSTRACT Aminotransferases, which catalyze the last step of biosynthesis of most amino acids and the first step of their catabolism, may be involved in the growth of Lactococcus lactis in milk. Previously, we isolated two aminotransferases from L. lactis, AraT and BcaT, which are responsible for the transamination of aromatic amino acids, branched-chain amino acids, and methionine. In this study, we demonstrated that double inactivation of AraT and BcaT strongly reduced the growth of L. lactis in milk. Supplementation of milk with amino acids and keto acids that are substrates of both aminotransferases did not improve the growth of the double mutant. On the contrary, supplementation of milk with isoleucine or a dipeptide containing isoleucine almost totally inhibited the growth of the double mutant, while it did not affect or only slightly affected the growth of the wild-type strain. These results suggest that AraT and BcaT play a major role in the growth of L. lactis in milk by degrading the intracellular excess isoleucine, which is responsible for the growth inhibition. The growth inhibition by isoleucine is likely to be due to CodY repression of the proteolytic system, which is necessary for maximal growth of L. lactis in milk, since the growth of the CodY mutant was not affected by addition of isoleucine to milk. Moreover, we demonstrated that AraT and BcaT are part of the CodY regulon and therefore are regulated by nutritional factors, such as the carbohydrate and nitrogen sources.

Effects of Branched-Chain α-Keto Acids on Plasma Amino Acid Concentrations in Carbon Tetrachloride-Intoxicated Rats

1984 ◽  
Vol 114 (7) ◽  
pp. 1235-1241 ◽  
Author(s):  
Misako Okita ◽  
Akiharu Watanabe ◽  
Nobuyuki Takei ◽  
Hideo Nagashima ◽  
Toshihiko Ubuka
Keyword(s):  
Amino Acid ◽  
Carbon Tetrachloride ◽  
Plasma Amino Acid ◽  
Keto Acids ◽  
Branched Chain ◽  
Amino Acid Concentrations
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