Protection against Superoxide and Hydrogen Peroxide in Synovial Fluid from Rheumatoid Patients

1981 ◽  
Vol 61 (4) ◽  
pp. 483-486 ◽  
Author(s):  
D. R. Blake ◽  
N. D. Hall ◽  
D. A. Treby ◽  
B. Halliwell ◽  
J. M. C. Gutteridge
Keyword(s):  
Hydrogen Peroxide ◽  
Synovial Fluid ◽  
Oxygen Radicals ◽  
Superoxide Radical ◽  
Joint Disease ◽  
Polymorphonuclear Leucocytes ◽  
Inflammatory Joint Disease ◽  
Normal Range ◽  
Lubricating Properties

1. On exposure of synovial fluid to superoxide and hydrogen peroxide, generated enzymically or by activated polymorphonuclear leucocytes, hyaluronic acid is depolymerized and the fluid loses its lubricating properties. The ability of synovial fluid from rheumatoid patients to scavenge superoxide and hydrogen peroxide was therefore examined. 2. Synovial fluid from a range of rheumatoid patients contained no superoxide dismutase activity, insufficient caeruloplasmin to scavenge any superoxide radical and little, if any, catalase activity. 3. Total ascorbate (reduced ascorbate + dehydroascorbate) concentrations in the plasma and synovial fluid of rheumatoid patients were similar in each case. The values are at the low end of the normal range. 4. These results are discussed in relation to the role of oxygen radicals in inflammatory joint disease.

An ESR study of the peroxidase reaction catalyzed by human methaemoglobin and methaemoglobin-haptoglobin complex

1991 ◽  
Vol 56 (4) ◽  
pp. 923-932
Author(s):  
Jana Stejskalová ◽  
Pavel Stopka ◽  
Zdeněk Pavlíček
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Peroxidase Activity ◽  
Amino Acid Analysis ◽  
Superoxide Radical ◽  
Esr Spectra ◽  
The Other ◽  
Delocalized Electron

The ESR spectra of peroxidase systems of methaemoglobin-ascorbic acid-hydrogen peroxide and methaemoglobin-haptoglobin complex-ascorbic acid-hydrogen peroxide have been measured in the acetate buffer of pH 4.5. For the system with methaemoglobin an asymmetrical signal with g ~ 2 has been observed which is interpreted as the perpendicular region of anisotropic spectrum of superoxide radical. On the other hand, for the system with methaemoglobin-haptoglobin complex the observed signal with g ~ 2 is symmetrical and is interpreted as a signal of delocalized electron. After realization of three repeatedly induced peroxidase processes the ESR signal of the perpendicular part of anisotropic spectrum of superoxide radical is distinctly diminished, whereas the signal of delocalized electron remains practically unchanged. An amino acid analysis of methaemoglobin along with results of the ESR measurements make it possible to derive a hypothesis about the role of haptoglobin in increasing of the peroxidase activity of methaemoglobin.

α-Tocopherol, lipids and lipoproteins in knee-joint synovial fluid and serum from patients with inflammatory joint disease

1992 ◽  
Vol 83 (6) ◽  
pp. 657-664 ◽  
Author(s):  
K. Fairburn ◽  
M. Grootveld ◽  
R. J. Ward ◽  
C. Abiuka ◽  
M. Kus ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Biologically Active ◽  
Joint Disease ◽  
Inflammatory Joint Disease ◽  
Serum Samples ◽  
Control Serum ◽  
Inflammatory Synovial Fluid

1. We have determined the antioxidant status of synovial fluid and serum of patients with inflammatory joint disease in terms of the biologically active lipid-soluble antioxidant, α-tocopherol. Synovial fluid concentrations of α-tocopherol were significantly lower relative to those of paired serum samples (P<0.001). Serum levels of α-tocopherol in these patients did not differ significantly from those in control serum. 2. Lower concentrations of cholesterol, triacylglycerol and low-density lipoprotein were also observed in patients' synovial fluid compared with matched serum samples. However, multiple regression analysis of the data indicated that there remained a significant depletion of α-tocopherol, which was largely independent of these co-variables, in inflammatory synovial fluid. These findings are consistent with the consumption of α-tocopherol within the inflamed joint via its role in terminating the process of lipid peroxidation. 3. Nuclear magnetic resonance spectroscopic analysis of matched inflammatory synovial fluid and serum confirmed lower concentrations of triacylglycerol in synovial fluid together with evidence of a shortened mean triacylglycerol chain length. The latter metabolic difference suggests an increased utilization of triacylglycerols for energy within the inflamed joint.

The multifaceted role of adiponectin in inflammatory joint disease

2009 ◽  
Vol 5 (12) ◽  
pp. 659-660 ◽  
Author(s):  
Ulf Müller-Ladner ◽  
Elena Neumann
Keyword(s):  
Joint Disease ◽  
Inflammatory Joint Disease

scholarly journals The role of leukocyte-stromal interactions in chronic inflammatory joint disease

2005 ◽  
Vol 72 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Angela Burman ◽  
Oliver Haworth ◽  
Paul Bradfield ◽  
Greg Parsonage ◽  
Andrew Filer ◽  
...  
Keyword(s):  
Joint Disease ◽  
Inflammatory Joint Disease

Comparative Analysis of CD4 Subsets in Peripheral Blood and Synovial Fluid of Patients with Inflammatory Joint Disease

1990 ◽  
Vol 19 (2) ◽  
pp. 157-161 ◽  
Author(s):  
A. D. Crockard ◽  
M. B. Finch ◽  
A. C. Fay ◽  
T. A. McNeill ◽  
A. L. Bell ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Synovial Fluid ◽  
Peripheral Blood ◽  
Joint Disease ◽  
Inflammatory Joint Disease

Decisive Diagnosis of Seronegative Polyarthritis by 3-phase Bone Scintigraphy

1998 ◽  
Vol 37 (07) ◽  
pp. 254-256 ◽  
Author(s):  
E. Wagner ◽  
P. Knoll ◽  
A. Dunky ◽  
H. Röhn ◽  
S. Mirzaei
Keyword(s):  
Clinical Investigation ◽  
Conventional Radiography ◽  
Joint Disease ◽  
Inflammatory Joint Disease ◽  
Acute Phase Reactants ◽  
X Ray ◽  
Three Phase ◽  
Seronegative Polyarthritis ◽  
Selection Of

SummaryInflammatory joint disease is mainly diagnosed on grounds of clinical investigation, laboratory testing (acute phase reactants), and radiography. Radionuclide imaging has recently been added to the armamentarium of clinician. This case report points out the role of three-phase bone scan and HIG (human immunoglobulin) scan in the discovery of the inflammatory nature of polyarthralgia in a young woman with equivocal clinical and laboratory results. In the aim of diagnosing arthritis early in its course scintigraphy proved to be superior to conventional radiography. It also allows more discriminating selection of subsequent X-ray examination to limit radiation exposure.

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