Fluorescent Lipid-Peroxidation Products in Synovial Fluid

1979 ◽  
Vol 56 (1) ◽  
pp. 53-59 ◽  
Author(s):  
J. Lunec ◽  
T. L. Dormandy
Keyword(s):  
Lipid Peroxidation ◽  
Synovial Fluid ◽  
Degradation Products ◽  
Free Radical Oxidation ◽  
Aqueous Methanol ◽  
Prolonged Incubation ◽  
Radical Oxidation ◽  
Polyunsaturated Lipids ◽  
Fluorescent Compounds

1. Samples and extracts from synovial effusions were examined for the presence of fluorescent degradation products of free-radical oxidation (peroxidation). 2. Two classes of fluorescent compounds were identified and their fluorescence and physical properties are described. The fluorescence in the aqueous methanol phase of the extracts had the characteristics of conjugated Schiff bases. 3. Changes in synovial fluid and synovial fluid extracts in vitro induced by u.v. irradiation, prolonged incubation and the enzymic generation of oxidizing free radicals, with or without admixed peroxidizing arachidonic acid, and in the absence and presence of antioxidants, suggest that the fluorescent material is derived from peroxidized polyunsaturated lipids in damaged synovial tissue.

Lipid Peroxidation in Iron-Overloaded Spleens

1981 ◽  
Vol 60 (3) ◽  
pp. 295-301 ◽  
Author(s):  
A. D. Heys ◽  
T. L. Dormandy
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Iron Overload ◽  
Iron Content ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Therapeutic Implications ◽  
Dose Dependent ◽  
Increased Susceptibility

1. Iron-overloaded spleens from thalassaemic subjects showed a greatly increased susceptibility to free-radical oxidation (peroxidation) in vitro. 2. Iron content was the main but not the only variable governing susceptibility. Added ascorbate had a dose-dependent action either as an antioxidant or as a pro-oxidant, the direction of its effect depending mainly on the degree of iron overload. This has therapeutic implications.

scholarly journals Reciprocity between muscarinic and nicotinic cholinoreactive structures of liver tissue in conditions, in substrates and in products of free radical oxidation of liver lipids in 5-day period of cooling in rats

2019 ◽  
Vol 17 (3) ◽  
pp. 51-58
Author(s):  
Viktor I. Tikhanov
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Membrane ◽  
Free Radical ◽  
Liver Tissue ◽  
Free Radical Oxidation ◽  
Liver Lipids ◽  
Radical Oxidation ◽  
Nicotinic Cholinoreceptors

The effects of administration of cholinotropic agents (neostigmine, hexamethonium, pilocarpine, atropine, nicotine) on liver tissue after 5-day period of cooling of rats were compared. The analysis of the conditions inducing lipid peroxidation (LPO), the analysis of the substrate components of the LPO in liver, and the evaluation of the LPO products content in 5-day period of cold loads were made. The data obtained indicate on the contradirectory effects when pilocarpine and nicotine were administered to animals, as well as after administration of atropine and hexamethonium to animals. There was the similar effects after administration of both pilocarpine and hexamethonium and both nicotine and atropine in conditions of activated LPO assessed by substrate components of LPO in the liver after 5-day cooling. On the basis of these results, a hypothesis of reciprocity between muscarinic and nicotinic cholinoreceptors located on the plasma membrane of hepatocytes in the LPO processes of the liver during the period of cold loads has been formulated.

scholarly journals Features of free radical lipid peroxidation and serum proteins at dental implants and peri-implantitis

2015 ◽  
Vol 96 (5) ◽  
pp. 756-759 ◽  
Author(s):  
D V Plyukhin ◽  
V E Tseylikman ◽  
O B Tseylikman ◽  
A I Sinitskiy
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Blood Serum ◽  
Dental Implants ◽  
Schiff Bases ◽  
Free Radical Oxidation ◽  
Protein Carbonylation ◽  
Radical Oxidation ◽  
Lipid Peroxidation Products ◽  
Dental Implantation

Aim. Determine the most informative indicators of free radical peroxidation in blood plasma for predicting the complications of dental implantation. Methods. The study included 84 patients of both with dental implants, including 31 patients without any complications and 53 patients with peri-implantitis. The levels of lipid peroxidation products and protein carbonylation were measured in peripheral blood. Results. Moderate intensification of lipid peroxidation and protein carbonylation was discovered in blood serum of patients with dental implants. Increased free radical oxidation in the blood was not limited to lipid peroxidation and resulted in increased serum levels of carbonylated proteins. Facilitation of oxidative degradation of proteins in the blood was associated with decreased resistance to «carbonyl stress», manifested as increased level of Fe+2/H2O2-induced carbonylation. Development of peri-implantitis resulted in a greater enhancement of free radical oxidation of blood. Isopropanol-soluble and heptane-soluble Schiff bases level was increased in blood serum as well. Similar to patients with dental implants, increased levels of carbonylated proteins was observed in blood serum at peri-implantitis. However, free radical oxidation was more intense in patients with peri-implantitis compared to patients with dental implants. Indeed, the level of heptane-soluble Schiff bases at the group with peri-implantitis was 2 times higher as compared to dental implants group, and the level of isopropanol-soluble lipid peroxidation products in inflammatory complications after implantation has reached fourfold increase compared with patients who underwent operation without complications. Conclusion. Investigated indicators of free radical oxidation may be used as markers of peri-implantitis. Among them, the most informative indicators are Schiff bases and metal-catalyzed protein oxidation.

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

scholarly journals Studying the processes of free-radical oxidation in patients with purulent-inflammatory diseases of soft tissues

2015 ◽  
Vol 96 (3) ◽  
pp. 302-306
Author(s):  
T Z Zakiev ◽  
S R Tuysin ◽  
O V Galimov ◽  
A R Gil’fanov ◽  
R D Sagdeev
Keyword(s):  
Free Radical ◽  
Whole Blood ◽  
Soft Tissues ◽  
Local Treatment ◽  
Free Radical Oxidation ◽  
Model Systems ◽  
Radical Oxidation ◽  
Tissue Homogenates

Aim. To study the effects of combined dressings on the processes of free radical oxidation in patients with purulent wounds.Methods. The performance of luminol-dependent chemiluminescence of whole blood from 30 healthy donors and 122 patients with purulent wounds, as well as tissue chemiluminescence of festering wounds tissue homogenates were examined before and after the treatment. To assess the free radical oxidation in vitro, spontaneous and zymosan-induced whole blood chemiluminescence measurement, iron-induced chemiluminescence assessment of festering wounds tissue homogenates were performed. The distribution of chemiluminescence parameters compared to normal expected distribution was analyzed to group homogeneity on these criteria.Results. Group of donors was homogeneous in composition that allowed the calculation of mean values. By changing in whole blood chemiluminescence of patients with soft tissue festering wounds, they were allocated to two groups, in which a marked increase or decline of the investigated parameters was seen. Increased luminol-dependent blood chemiluminescence indicates excessive free radicals generation by phagocytes and is characteristic of acute inflammation. Decreased intensity of chemiluminescence was observed in the blood of patients who showed reduced functional activity of phagocytes, which, together with the clinical features indicate a long-term smoldering inflammation. The comparative assessment of «Poliderm», «Voskopran», «Polysorb», «Levomekol» medications, used for local treatment of suppurative wounds, influence on free radical oxidation in model systems in vitro, was performed.Conclusion. There is a triple increase in chemiluminescence intensity in the acute phase of the inflammatory process, marking increased production of reactive oxygen radicals with microbicide activity); in long-term smoldering processes, chemiluminescence decreases by half, indicating reduced efficiency of protective mechanisms

scholarly journals Seasonal survey of the composition and degradation state of particulate organic matter in the Rhone River using lipid tracers

2014 ◽  
Vol 11 (10) ◽  
pp. 14197-14237 ◽  
Author(s):  
M.-A. Galeron ◽  
R. Amiraux ◽  
B. Charriere ◽  
O. Radakovitch ◽  
P. Raimbault ◽  
...  
Keyword(s):  
Organic Matter ◽  
Particulate Organic Matter ◽  
Degradation Products ◽  
Significant Proportion ◽  
Free Radical Oxidation ◽  
Bacterial Degradation ◽  
Higher Plant ◽  
Rhône River ◽  
Radical Oxidation ◽  
Rhone River

Abstract. Lipid tracers including fatty acids, hydroxyacids, n-alkanols, sterols and triterpenoids were used to determine the origin and fate of suspended particulate organic matter (POM) collected in the Rhone River (France). This seasonal survey (April 2011 to May 2013) revealed a year-round strong terrigenous contribution to the plant-derived particulate organic matter (POM), with significant algal inputs observed in March and attributed to phytoplanktonic blooms likely dominated by diatoms. Major terrigenous contributors to our samples are gymnosperms, and more precisely their roots and stems, as evidenced by the presence of high proportions of ω-hydroxydocosanoic acid (a suberin biomarker). The high amounts of coprostanol detected clearly show that the Rhone River is significantly affected by sewage waters. Specific sterol degradation products were quantified and used to assess the part of biotic and abiotic degradation of POM within the river. Plant-derived organic matter appears to be mainly affected by photo-oxidation and autoxidation (free radical oxidation), while organic matter of human origin, evidenced by the presence of coprostanol, is clearly more prone to bacterial degradation. Despite the involvement of an intense autoxidation-inducing homolytic cleavage of peroxy bonds, a significant proportion of hydroperoxides is still intact in higher plant debris. These compounds could affect the degradation of terrestrial material by inducing an intense autoxidation upon its arrival at sea.

scholarly journals Seasonal survey of the composition and degradation state of particulate organic matter in the Rhône River using lipid tracers

2015 ◽  
Vol 12 (5) ◽  
pp. 1431-1446 ◽  
Author(s):  
M.-A. Galeron ◽  
R. Amiraux ◽  
B. Charriere ◽  
O. Radakovitch ◽  
P. Raimbault ◽  
...  
Keyword(s):  
Organic Matter ◽  
Particulate Organic Matter ◽  
Degradation Products ◽  
Significant Proportion ◽  
Free Radical Oxidation ◽  
Bacterial Degradation ◽  
Higher Plant ◽  
Rhône River ◽  
Radical Oxidation ◽  
Rhone River

Abstract. Lipid tracers including fatty acids, hydroxyacids, n-alkanols, sterols and triterpenoids were used to determine the origin and fate of suspended particulate organic matter (POM) collected in the Rhône River (France). This seasonal survey (April 2011 to May 2013) revealed a year-round strong terrestrial higher-plant contribution to the particulate organic matter (POM), with significant algal inputs observed in March and attributed to phytoplanktonic blooms likely dominated by diatoms. Major terrigenous contributors to our samples are gymnosperms, and more precisely their roots and stems, as evidenced by the presence of high proportions of ω-hydroxydocosanoic acid (a suberin biomarker). The high amounts of coprostanol detected clearly show that the Rhône River is significantly affected by sewage waters. Specific sterol degradation products were quantified and used to assess the part of biotic and abiotic degradation of POM within the river. Higher-plant-derived organic matter appears to be mainly affected by photo-oxidation and autoxidation (free radical oxidation), while organic matter of mammal or human origin, evidenced by the presence of coprostanol, is clearly more prone to bacterial degradation. Despite the involvement of an intense autoxidation-inducing homolytic cleavage of peroxy bonds, a significant proportion of hydroperoxides is still intact in higher plant debris. These compounds could affect the degradation of terrestrial material by inducing an intense autoxidation upon its arrival at sea.

scholarly journals Specific features of biological oxidation processes in helminthiases of horses

2020 ◽  
Vol 61 (3) ◽  
pp. 126-130
Author(s):  
Larisa V. Pilip ◽  
◽  
Olga V. Byakova ◽  
Maria E. Kazakova ◽  
◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The Body ◽  
Luminous Intensity ◽  
Radical Oxidation ◽  
Pathological Conditions ◽  
Oxidized Products

The chemical processes occurring in the animal body under pathological conditions are of interest to researchers. The objective of the research was to study the indicators of lipid peroxidation and antioxidant activity in horses infested with helminthiases and treated with anthelmintic paste with an antioxidant. The study has proved the role of parasitic infestation in accelerating the processes of free radical oxidation, which is manifested by a decrease in the light sum of radicals (36.62-39.21), the maximum luminous intensity (6.13-6.98) and antioxidant activity (5.65-6.2) at the start of the research. Chronic invasion increases the concentration of under-oxidized products up to 38.59, and the rate of initiating free radical oxidation – up to 6.95, the values of antioxidant activity being stably low (5.51-5.91). An important role in the regulation of lipid peroxidation in horses during helminthiases, as well as in the process of deworming, is played by the antioxidants contained in anthelmintic preparations. The antioxidant santohin included as a compound in the «Alezan» preparation suppresses enhanced peroxidation and activates the antioxidant protection of the body, which is reflected in a decrease in the light sum of the radicals to as low as 26.73, a maximum luminous intensity to – 3.76, and an increase in antioxidant activity – up to 7.16.

Sign in / Sign up

Export Citation Format

Share Document