The Effect of the Porphyrogenic Compound, Hexachlorobenzene, on the Activity of Hepatic Uroporphyrinogen Decarboxylase in the Rat

1976 ◽  
Vol 51 (1) ◽  
pp. 71-80 ◽  
Author(s):  
G. H. Elder ◽  
J. O. Evans ◽  
S. A. Matlin
Keyword(s):  
Rat Liver ◽  
Wistar Rats ◽  
New Method ◽  
Decarboxylase Activity ◽  
Uroporphyrinogen Decarboxylase ◽  
Enzyme Defect ◽  
Female Wistar Rats ◽  
Liver Homogenates

1. A new method for the measurement of uroporphyrinogen decarboxylase (EC 4.1.1.37) in rat liver homogenates, with 5-carboxyl porphyrinogen as substrate, is described. 2. The administration of a diet containing 0·3% (w/w) hexachlorobenzene produces porphyria in female Wistar rats after a delay of at least 4 weeks. The development of porphyria is accompanied by a progressive fall in hepatic uroporphyrinogen decarboxylase activity to 18% of control values after 11 weeks. The features of hexachlorobenzene porphyria are consequences of this enzyme defect. 3. Feeding with hexachlorobenzene did not lead to the accumulation of iron in the liver. It is suggested that hexachlorobenzene or a metabolite acts directly to decrease the activity of the enzyme.

scholarly journals Investigations of rat liver uroporphyrinogen decarboxylase. Comparisons of porphyrinogens I and III as substrates and the inhibition by porphyrins

1981 ◽  
Vol 195 (1) ◽  
pp. 241-250 ◽  
Author(s):  
A G Smith ◽  
J E Francis
Keyword(s):  
Rat Liver ◽  
Rapid Analysis ◽  
Oxidation Products ◽  
Decarboxylase Activity ◽  
Effective Inhibitor ◽  
Uroporphyrinogen Decarboxylase ◽  
The Difference ◽  
Substrate Concentrations ◽  
Slow Turnover

1. The decarboxylations of uroporphyrinogens, hepta-, hexa- and penta-carboxyporphyrinogens I and III by porphyrinogen carboxy-lyase (EC 4.1.1.37) in rat liver supernatant have been compared as functions of substrate concentrations. Although Km and Vmax. (for total porphyrinogens formed) were estimated, prophyrinogens and CO2 produced at 1 microM were considered to be a better indication of real relative rates, owing to substrate/product inhibitions. Uroporphyrinogen III was the best substrate by the criteria of Km/Vmax. and decarboxylation at 1 microM and was converted into coproporphyrinogen more quickly than its series-I isomer. 2. The difference between uroporphyrinogens I and III as substrates was confirmed by using a mixture of [14C8]uroporphyrinogens, the discrimination occurring principally in the first decarboxylation. 3. Porphyrins, especially oxidation products of the substrates, inhibited the enzyme. Heptacarboxyporphyrin III was the most effective inhibitor of both uroporphyrinogen III and heptacarboxyporphyrinogen III conversion into coproporphyrinogen. 4. Rapid analysis of the livers from rats made porphyric with hexachlorobenzene demonstrated that substantial quantities of the tetrapyrroles were present in vivo as the porphyrinogens (21-42%). 5. Enzymic decarboxylation of uroporphyrinogen III in 2H2O-containing buffer gave [2H4]coproporphyrinogen. 6. Rats treated with cycloheximide for 10h showed no decrease in uroporphyrinogen decarboxylase activity/mg of protein, suggesting a relatively slow turnover of the enzyme.

Alcohol-Induced Decrease in Uroporphyrinogen Decarboxylase Activity in Rat Liver and Spleen

Enzyme ◽  
1981 ◽  
Vol 26 (1) ◽  
pp. 24-31 ◽  
Author(s):  
M. Doss ◽  
R. von Tiepermann ◽  
G. Stutz ◽  
R. Teschke
Keyword(s):  
Rat Liver ◽  
Decarboxylase Activity ◽  
Uroporphyrinogen Decarboxylase

scholarly journals Liquid-base cytology: a new method for oestral cycle study in wistar's rats

2005 ◽  
Vol 20 (suppl 1) ◽  
pp. 46-49 ◽  
Author(s):  
Rand Randall Martins ◽  
Ney Moura Lemos Pereira ◽  
Telma Maria Araújo Silva
Keyword(s):  
Evans Blue ◽  
Wistar Rats ◽  
New Method ◽  
Hormonal Cycle ◽  
Female Wistar Rats ◽  
Clear Differentiation ◽  
Cellular Types ◽  
Ether Alcohol

OBJECTIVES: The objective of this study was the standardization of a collection technique and staining in liquid-base that allies the pratical and cytological wealth, making possible a larger reproductibility and microscopic easiness. METHODS: Female wistar rats (n=20) were submitted to the daily vaginal collection in saline and fastened washed (ether/alcohol) and stained in suspension with a solution of Evans Blue 0.025%. The sample was pondered by centrifugation and observed under lens of 40 x. RESULTS: The stained smears allowed clear differentiation of the phases of hormonal cycle (diestrus, proestrus, estrus and metestrus); besides the differentiation of the cellular types in relation to its maturation degree having as parameters the cellular size, nucleus / cytoplasm relationship (NCR) and ink reaction. The study demonstrated the existence of three basic cellular patterns: cells with low NCR, accentuated cyanophily and small size; cells with increment in NCR, cyanophilic loss and larger volume cytoplasmatic and without nuclei keratinization cells in squamous aspect. CONCLUSION: The staining of the material allowed, besides the cytological classification, the quantification possibility that would result in a perfected accompaniment of the cycle estrous.

NEW DATA CONCERNING THE ROLE PLAYED BY PROGESTERONE IN THE CONTROL OF FOLLICULAR GROWTH IN THE RAT

1974 ◽  
Vol 75 (3) ◽  
pp. 569-578 ◽  
Author(s):  
G. Buffler ◽  
S. Roser
Keyword(s):  
Wistar Rats ◽  
Venous Blood ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Cycle Duration ◽  
Follicular Growth ◽  
The Third ◽  
Female Wistar Rats

ABSTRACT The mechanisms involved in the prolongation of the oestrous cycle following LH administration were studied in 4-day cyclic female Wistar rats. In females injected with LH on the morning of dioestrus I there was an increase in ovarian venous blood progesterone as compared with non-injected animals. In both LH-treated females, and those injected with progesterone on the morning of dioestrus I, a slowing up in follicular growth was observed from the afternoon of dioestrus I. The size of follicles greater than 400 urn present in LH or progesterone injected animals on the third day of cycle was similar to the size reached by the same range of follicles in non-injected animals on the second day of the cycle. Hence, the increase in endogenous ovarian progesterone elicited by LH was considered as the cause of the slowing up of follicular growth and therefore of the lengthening of the oestrous cycle duration in female rats injected with LH at the beginning of 4-day cycle.

Oral in vivo Bactofection in Dextran Sulfate Sodium Treated Female Wistar Rats

2010 ◽  
Vol 58 (3) ◽  
pp. 171-176 ◽  
Author(s):  
Roland Pálffy ◽  
Michal Behuliak ◽  
Roman Gardlík ◽  
Peter Jáni ◽  
L'udevít Kádaši ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Female Wistar Rats
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