Dopa, Catecholamines and Their Metabolites in Essential Hypertension

1974 ◽  
Vol 48 (s2) ◽  
pp. 295s-298s
Author(s):  
W. Januszewicz ◽  
B. Wocial
Keyword(s):  
Essential Hypertension ◽  
Urinary Excretion ◽  
Homovanillic Acid ◽  
Catecholamine Metabolism ◽  
Vanillylmandelic Acid ◽  
Control Subjects ◽  
Healthy Control ◽  
Catecholamine Metabolites ◽  
The Relationship

1. Urinary excretion of dopa, catecholamines and their metabolites (vanillylmandelic acid, methoxycatecholamines, 3-methoxy-4-hydroxyphenylglycol and homovanillic acid) were studied in eighty patients with essential hypertension and in twenty-five healthy control subjects. 2. Increased urinary excretion of catecholamines, dopa and catecholamine metabolites was found in a proportion of cases. 3. The relationship between urinary excretion of catecholamine metabolites and the excretion of dopa and noradrenaline was studied. 4. In view of the suggested significance of 3-methoxy-4-hydroxyphenylglycol as an index of brain catecholamine metabolism, particular attention was paid to urinary excretion of this metabolite in the subjects under study.

EXCRETION OF CATECHOLAMINE METABOLITES BY CHILDREN WITH FAMILIAL DYSAUTONOMIA

PEDIATRICS ◽  
1970 ◽  
Vol 46 (4) ◽  
pp. 513-522
Author(s):  
Stanley E. Gitlow ◽  
Laura M. Bertani ◽  
Elizabeth Wilk ◽  
Biao Lan Li ◽  
Stanley Dziedzic
Keyword(s):  
Homovanillic Acid ◽  
Matched Control ◽  
Normal Subjects ◽  
Biochemical Parameter ◽  
Familial Dysautonomia ◽  
Catecholamine Metabolism ◽  
Vanillylmandelic Acid ◽  
Sensitive Assay ◽  
Specific Nature ◽  
Catecholamine Metabolites

Although a defect in catecholamine metabolism has been suggested in familial dysautonomia, the specific nature and extent of this abnormality has not been defined. Highly specific and sensitive assay techniques were used to measure the excretion of vanillylmandelic acid (VMA), 3-methoxy-4-hydroxyphenylethyleneglycol (HMPG), total metanephrines (TM), normetanephrine (NM), and homovanillic acid (HVA) of 52 dysautonomic patients and 180 normal subjects. Twenty parents of dysautonomic patients and age-matched control subjects were studied similarly. Patients with familial dysautonomia were found to excrete elevated quantities of HVA, diminished quantities of VMA and HMPG, and normal amounts of TM and NM in comparison with normal subjects. The excretion of the sum of the deaminated catecholamine metabolites proved to be more aberrant in familial dysautonomia than any other biochemical parameter measured. The findings of this study are more compatible with an abnormality in catecholamine synthesis rather than in release. Parents of dysautonomic subjects excreted normal amounts of vanillylmandelic acid and homovanillic acid.

scholarly journals Neutrophil-to-Lymphocyte and Platelet-to-Lymphocyte Ratios in Patients with RetinalArtery Occlusion

2020 ◽  
Author(s):  
Mahmut Atum ◽  
Gürsoy Alagöz
Keyword(s):  
Retinal Artery Occlusion ◽  
Artery Occlusion ◽  
Control Group ◽  
Retrospective Case ◽  
Control Subjects ◽  
Gender And Age ◽  
Significant Difference ◽  
Healthy Control ◽  
Retinal Artery ◽  
The Relationship

Purpose: This study aimed to compare the neutrophil-to-lymphocyte (NLR) and plateletto- lymphocyte (PLR) ratios in patients with retinal artery occlusion (RAO) with those from a healthy control population and to identify the relationship between them. Methods: Forty-six patients with RAO and fifty-one healthy control subjects were included in this retrospective case-control study. RAO was diagnosed following an ophthalmic examination and fluorescein angiography (FA). Blood neutrophil, lymphocyte, and platelet counts were recorded for each of the 97 subjects, from which NLR and PLR values were calculated. Results: There were 46 patients (28 male [M], 18 female [F]) in the RAO group and 51 patients (27 M, 24 F) in the control group. No significant differences were found between patients with RAO and the control subjects in terms of gender and age (P > 0.05). Patients with RAO had significantly increased NLR values (2.85 ± 1.70) than the control subjects (1.63 ± 0.59, P < 0.001). The mean PLR in patients with RAO was 123.69 ± 64.98, while that in control subjects was 103.08 ± 36.95; there was no significant difference between the two groups (P = 0.055). A logistic regression analysis revealed that NLRs were 3.8 times higher in patients with RAO than in control subjects (odds ratio = 3.880; 95% confidence interval = 1.94 to 7.74; P < 0.001). Conclusion: NLRs were significantly increased in patients with RAO compared to the control subjects.

The Effect of Mental Stress on Catecholamines, their Metabolites and Plasma Renin Activity in Patients with Essential Hypertension and in Healthy Subjects

1979 ◽  
Vol 57 (s5) ◽  
pp. 229s-231s ◽  
Author(s):  
W. Januszewicz ◽  
M. Sznajderman ◽  
B. Wocial ◽  
T. Feltynowski ◽  
T. Klonowicz
Keyword(s):  
Essential Hypertension ◽  
Plasma Renin Activity ◽  
Urinary Excretion ◽  
Mental Stress ◽  
Healthy Subjects ◽  
Plasma Renin ◽  
Renin Activity ◽  
Vanillylmandelic Acid ◽  
Before And After ◽  
Noradrenaline And Adrenaline

1. Ten patients with essential hypertension and ten healthy men were submitted to mental stress consisting of Kraepelin's arithmetic test combined with noise. Concentrations of plasma and urine catecholamines and of their metabolites as well as plasma renin activity before and after the test were studied. 2. In both groups a significant increase of noradrenaline and adrenaline in blood and noradrenaline in urine was observed. The urinary excretion of dopamine fell significantly in both groups after stress. 3. After mental stress a significant increase in urinary excretion of 3-methoxy-4-hydroxyphenylglycol was observed in both groups. The excretion of vanillylmandelic acid decreased significantly only in healthy subjects. 4. The plasma renin activity rose significantly in both groups but the increase was more pronounced in healthy subjects.

scholarly journals The Serum High-Sensitive C Reactive Protein and Homocysteine Levels to Evaluate the Prognosis of Acute Ischemic Stroke

2007 ◽  
Vol 2007 ◽  
pp. 1-5 ◽  
Author(s):  
Tahir Yoldas ◽  
Murat Gonen ◽  
Ahmet Godekmerdan ◽  
Fulya Ilhan ◽  
Ednan Bayram
Keyword(s):  
Ischemic Stroke ◽  
Clinical Status ◽  
Elevated Serum ◽  
Short Term ◽  
Reactive Protein ◽  
Control Subjects ◽  
Common Causes ◽  
Healthy Control ◽  
The Mean ◽  
The Relationship

Ischemic stroke is one of the most common causes of death worldwide and is most often caused by thrombotic processes. We investigated the changes in hsCRP and homocysteine levels, two of these risk factors, during the acute period of ischemic stroke and evaluated the relationship between these levels and the short-term prognosis. HsCRP and homocysteine levels were measured at the 2nd, 5th, and 10th days in forty patients admitted within second of an ischemic stroke. The clinical status of the patients was simultaneously evaluated with the Scandinavian stroke scale. The results were compared with 40 healthy control subjects whose age and sex were matched with the patients. The mean hsCRP levels of the patients were9.4±7.0mg/L on the 2nd day,11.0±7.4mg/L on the 5th day, and9.2±7.0mg/L on the 10th day. The mean hsCRP level of the control subjects was1.7±2.9mg/L. The mean hsCRP levels of the patients on the 2nd, 5th, and 10th days were significantly higher than the control subjects (P<.001). The patients' mean homocysteine levels were40.6±9.6μmol/L on the 2nd day,21.7±11.1μmol/L on the 5th day, and20.7±9.2μmol/L on the 10th day. The mean homocysteine level of the control subjects was11.2±1.1μmol/L. The homocysteine levels of the patients were higher than the control subjects at all times (P<.01). In conclusion, patients with stroke have a higher circulating serum hsCRP and homocysteine levels. Short-term unfavorable prognosis seems to be associated with elevated serum hsCRP levels in patients with stroke. Although serum homocysteine was found to be higher, homocysteine seems not related to prog nosis.

Identical Effects of Indomethacin on Renal Function in Healthy Uninephrectomized Subjects and in Healthy Control Subjects

1994 ◽  
Vol 86 (6) ◽  
pp. 715-721 ◽  
Author(s):  
C. B. Nielsen ◽  
S. S. Sørensen ◽  
E. B. Pedersen
Keyword(s):  
Renal Function ◽  
Urinary Excretion ◽  
Prostaglandin E2 ◽  
Excretion Rate ◽  
Control Group ◽  
Urinary Excretion Rate ◽  
Control Subjects ◽  
Time Control ◽  
Healthy Control ◽  
Remnant Kidney

1. Animal studies have shown that prostaglandins are important for renal function after unilateral nephrectomy. In order to investigate the importance of prostaglandins for renal function in the fully adapted remnant kidney in healthy uninephrectomized subjects, the acute effects of indomethacin on renal haemodynamics, lithium clearance, urinary excretion rates of prostaglandin E2, sodium and water, and plasma levels of angiotensin II, aldosterone, atrial natriuretic peptide and arginine vasopressin were measured in 14 healthy uninephrectomized subjects (median time after nephrectomy 1.7 years) and in 14 matched healthy control subjects. In addition, nine healthy control subjects were studied without indomethacin and served as a time-control group. 2. Before indomethacin ingestion there was a significantly higher single-kidney urinary excretion rate of prostaglandin E2 in the uninephrectomized group (uninephrectomized group, 349.2 fmol/min; control group, 76.6 fmol/min; time-control group, 96.3 fmol/min). 3. Indomethacin ingestion resulted in equal changes in all parameters in both groups. These were significant decreases in glomerular filtration rate (−11.3% versus −14.6%), renal plasma flow (−6.5% versus −13.0%), urinary flow rate (−49.8% versus −49.4%), fractional sodium excretion (−44.5% versus −47.4%), lithium clearance (−33.2% versus −23.8%) and urinary excretion rate of prostaglandin E2 (−93.8% versus −86.7%) (uninephrectomized versus control subjects, values are medians). In the time-control group no changes were observed in these parameters. 4. It is concluded that healthy uninephrectomized subjects with a fully adapted remnant kidney have a normal renal response to acute indomethacin-induced inhibition of prostaglandin synthesis.

Urinary Excretion of 20-Hete, Natriuresis and Salt-Sensitivity of Blood Pressure in Essential Hypertension

Hypertension ◽  
2000 ◽  
Vol 36 (suppl_1) ◽  
pp. 716-716
Author(s):  
Cheryl L Laffer ◽  
Michal Laniado-Schwartzman ◽  
Mong-Heng Wang ◽  
Alberto Nasjletti ◽  
Fernando Elijovich
Keyword(s):  
Blood Pressure ◽  
Essential Hypertension ◽  
Urinary Excretion ◽  
Salt Sensitivity ◽  
Nacl Transport ◽  
Salt Loading ◽  
Pm 10 ◽  
Salt Sensitive Hypertension ◽  
The Relationship ◽  
Na Depletion

P127 20-hydroxyeicosatetraenoic acid (20-HETE) inhibits NaCl transport in the loop of Henle. A deficit in its synthesis or action has been implicated in experimental salt-sensitive hypertension. We examined the relationship between urinary excretion of Na (UNaV) and 20-HETE in 26 essential hypertensive patients classified as salt-sensitive (SS=13) or resistant (SR=13) by an in-patient protocol of 24 hr salt-loading (HI: 160 mEq Na diet+2L saline iv) followed by 24-hr sodium deprivation (LO: 10 mEq Na diet+furosemide 120 mg in first 12 hrs). SS was defined as a fall in ambulatory systolic BP (SBP, noon to 10 pm)≥10 mmHg from HI to LO. The Table shows data in HI and in the second 12 hr period of LO, i.e., after Na depletion was achieved by furosemide. SS and SR did not differ in any of these data. 20-HETE did not correlate with race, age, or GFR. During HI, it showed a negative correlation with BMI (r=-0.44, p<0.03) and a positive one with BP (r=+0.42, p<0.04). Also, 20-HETE correlated with UNaV in all patients (r=+0.45, p<0.02), but this was due to a strong correlation in SR (r=+0.62, p<0.03), absent in SS. In LO, no correlations were observed between any of these measurements. Our data demonstrate that 20-HETE excretion varies with the state of salt-balance in human hypertension and diminishes with increasing obesity. Although excretion of 20-HETE is not different between SS and SR, there is a major difference in the relationship between excretion of this eicosanoid and natriuresis between these groups. Our data suggest that salt-sensitivity of blood pressure in human essential hypertension may result from impairment in a natriuretic mechanism dependent on 20-HETE.

Evidence for a pathological reduction in brain dopamine metabolism in idiopathic hyperprolactinemia

1991 ◽  
Vol 125 (3) ◽  
pp. 246-252 ◽  
Author(s):  
Roberto Paradisi ◽  
Gabriele Grossi ◽  
Angela Pintore ◽  
Stefano Venturoli ◽  
Eleonora Porcu ◽  
...  
Keyword(s):  
High Performance ◽  
Homovanillic Acid ◽  
Dopamine Metabolism ◽  
Vanillylmandelic Acid ◽  
Brain Dopamine ◽  
Urinary Catecholamines ◽  
Control Subjects ◽  
Dihydroxyphenylacetic Acid ◽  
Early Follicular Phase ◽  
Urinary Dopamine

Abstract. The role of brain catecholamine activity in the neuroendocrine regulation of the dopamine-PRL system in idiopathic hyperprolactinemia was investigated by high-performance liquid chromatography with electro-chemical detector. We measured urinary dopamine, norepinephrine, epinephrine, vanillylmandelic acid, homovanillic acid, 3,4-dihydroxyphenylacetic acid and total 3-methoxy-4-hydroxyphenylglycol levels in 12 women with idiopathic hyperprolactinemia before and during either peripheral dopa-decarboxylase blockade, by carbidopa, or dopamine β-hydroxylase blockade, by disulfiram. Homovanillic acid and 3,4-dihydroxyphenylacetic acid concentrations were significantly lower (p<0.001 and p<0.005, respectively) in patients with idiopathic hyperprolactinemia compared with those in 12 control subjects in the early follicular phase, whereas they were similar to those in the control subjects in the pre-ovulatory phase. Dopamine, norepinephrine, epinephrine, vanillylmandelic acid and 3-methoxy-4-hydroxyphenylglycol concentrations were similar to those of the control subjects in both phases of the cycle. During carbidopa administration the levels of all urinary catecholamines and metabolites were unchanged, except that of dopamine which dropped remarkably (p<0.001). During disulfiram administration dopamine, homovanillic acid and 3,4-dihydroxyphenylacetic acid concentrations increased (p<0.05, p<0.001 and p<0.005, respectively) and those of norepinephrine, vanillylmandelic acid and 3-methoxy-4-hydroxyphenylglycol decreased (p<0.05, p<0.001 and p<0.005, respectively), whereas epinephrine levels remained unaltered. These data support the existence of a quantitatively reduced brain dopamine activity in idiopathic hyperprolactinemia.

Simple liquid-chromatographic measurement of vanillylmandelic acid and homovanillic acid in urine on filter paper for mass screening of neuroblastoma in infants.

1987 ◽  
Vol 33 (11) ◽  
pp. 2043-2046 ◽  
Author(s):  
J Hanai ◽  
T Kawai ◽  
Y Sato ◽  
N Takasugi ◽  
M Nishi ◽  
...  
Keyword(s):  
Mass Screening ◽  
Filter Paper ◽  
High Performance ◽  
Homovanillic Acid ◽  
Screening Program ◽  
Simple Liquid ◽  
Vanillylmandelic Acid ◽  
Microtiter Plates ◽  
Catecholamine Metabolites ◽  
Liquid Chromatographic

Abstract We describe a simple and rapid method for measuring catecholamine metabolites in urine collected on filter paper. By this method, "high-performance" liquid chromatography, urinary vanillylmandelic acid and homovanillic acid can be measured within 15 min after being eluted from filter paper treated with tartrate buffer. One hundred urine samples can be pretreated within 2 h and 100-120 samples analyzed at 18-min intervals overnight in two chromatographic systems. Moreover, urinary creatinine also can be measured rapidly because of the use of microtiter plates. We applied this method in a mass screening program for early detection of neuroblastoma in infants. Between April 1986 and March 1987, two patients with neuroblastoma were detected. In all, neuroblastoma has been detected in 17 of 88,887 infants so examined since April 1981.

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