The Characteristics of Ferritin from Human Tissues, Serum and Blood Cells

1975 ◽  
Vol 48 (5) ◽  
pp. 441-451 ◽  
Author(s):  
M. Worwood ◽  
W. Aherne ◽  
S. Dawkins ◽  
A. Jacobs
Keyword(s):  
Iron Overload ◽  
Anion Exchange ◽  
Serum Ferritin ◽  
Blood Cells ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Normal Subjects ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Anion Exchange Column

1. The properties of ferritin in serum have been compared with those of ferritin from a number of tissues including blood cells. On anion-exchange chromatography with DEAE-Sephadex, the behaviour of human heart ferritin is different from that of liver, kidney or spleen ferritin. Reticulocyte ferritin appears to have similar characteristics to heart ferritin. 2. Serum ferritin from normal subjects and patients with various degrees of iron load, leukaemia or liver disease all have a much lower affinity for the anion-exchange column than any tissue ferritin, suggesting a difference in isoelectric point. The elution point of serum ferritin from patients with acute myeloblastic leukaemia is significantly different from normal. 3. Density gradient centrifugation in sucrose showed that ferritin in leucocyte extracts and partially purified ferritin from the serum of two patients with iron overload behaved as apoferritin rather than the iron-rich protein. 4. The results suggest that ferritin is modified during its entry into the plasma and that even in cases of iron overload the iron content of serum ferritin may be low. The findings are of importance in considering the origin of plasma ferritin, the clearance of ferritin from plasma and its role in iron metabolism.

Sexual dimorphism in immunoneutralization of bioactivity of rat and ovine inhibin

1986 ◽  
Vol 111 (2) ◽  
pp. 255-261 ◽  
Author(s):  
S. van Dijk ◽  
J. Steenbergen ◽  
J. Th. Gielen ◽  
F. H. de Jong
Keyword(s):  
Anion Exchange ◽  
Follicular Fluid ◽  
Culture Media ◽  
Anion Exchange Chromatography ◽  
Rete Testis ◽  
Exchange Chromatography ◽  
Pituitary Cells ◽  
Anion Exchange Column ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

ABSTRACT Inhibin was partially purified from bovine follicular fluid using chromatography on immobilized Procion Red 3B and anion-exchange chromatography. Ovariectomized Texel ewes were immunized against the inhibin-containing fraction from the Procion Red 3B column and the immune response was subsequently boosted with similar fractions or with the preparation obtained from the anion-exchange column. The potencies of the resulting antisera were evaluated in an invitro bioassay system for estimating inhibin activity, using dispersed rat pituitary cells. The antisera were found to inhibit the bioactivity of inhibin preparations from ovarian follicular fluid of bovine, porcine, ovine or human origin, as well as inhibin activity in ovine testicular lymph and rete testis fluid, in culture media from rat granulosa and rat Sertoli cells and in homogenates of rat ovaries and testes. These results indicate that the inhibin molecules from several species contain a common bioactive moiety. The results also showed that the antiserum was more effective in neutralizing inhibin activity from ovarian than from testicular sources in both sheep and rat, indicating a sex-related difference in the inhibin molecules within a species. J. Endocr. (1986) 111, 255–261

scholarly journals The purification and properties of ferritin from human serum

1976 ◽  
Vol 157 (1) ◽  
pp. 97-103 ◽  
Author(s):  
M Worwood ◽  
S Dawkins ◽  
M Wagstaff ◽  
A Jacobs
Keyword(s):  
Anion Exchange ◽  
Serum Ferritin ◽  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Partial Purification ◽  
Human Spleen ◽  
Human Ferritin

1. Ferritin has been isolated from the serum of four patients with iron overload by using two methods. 2. In method A, the serum was adjusted to pH 4.8 and heated to 70 degrees C. After removal of denatured protein, ferritin was concentrated and further purified by ion-exchange chromatography and gel filtration. In most cases, only a partial purification was achieved. 3. In method B, ferritin was extracted from the serum with a column of immuno-adsorbent [anti-(human ferritin)] and released from the column with 3M-KSCN. Further purification was achieved by anion-exchange chromatography followed by the removal of remaining contaminating serum proteins by means of a second immunoadsorbent. Purifications of up to 31 000-fold were achieved, and the homogeneity of the final preparations was demonstrated by polyacrylamide-gel electrophoresis. 4. Serum ferritin purified by either method has the same elution volume as human spleen ferritin on gel filtration on Sephadex G-200. Serum ferritin has a relatively low iron content and iron/protein ratios of 0.023 and 0.067 (mug of Fe/mug of protein) were found in two pure preparations. On anion-exchange chromatography serum ferritin has a low affinity for the column when compared with various tissue ferritins. Isoelectric focusing has demonstrated the presence of a high proportion of isoferritins of relatively high pI. 5. Possible mechanisms for the release of ferritin into the circulation are briefly discussed.

Simultaneous Determination of Fluoroacetates, Chloroacetates, and Bromoacetates in Soil Samples by Ion Chromatography

2004 ◽  
Vol 57 (10) ◽  
pp. 1005 ◽  
Author(s):  
Fang Wang ◽  
Greg W. Dicinoski ◽  
Yan Zhu ◽  
Paul R. Haddad
Keyword(s):  
Simultaneous Determination ◽  
Anion Exchange ◽  
Gradient Elution ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Soil Samples ◽  
Haloacetic Acids ◽  
Detection Methods ◽  
Anion Exchange Column

A comparative study was made of the chromatographic behaviour of nine haloacetic acids, namely mono-, di-, and tri-fluoroacetate (MFA, DFA, and TFA, respectively); mono-, di-, and tri-chloroacetate (MCA, DCA, and TCA, respectively); and mono-, di- and tri-bromoacetate (MBA, DBA, and TBA, respectively), employing anion-exchange chromatography with suppressed conductivity and UV detection, using a Dionex AS17 anion-exchange column employed with a potassium hydroxide gradient (via a Dionex EG40 Eluent Generator). All nine haloacetic acids were completely separated under the optimized gradient elution conditions, and use of selective detection methods or pretreatment with an OnGuard II Ag cartridge resulted in the elimination of interferences from chloride and bromide occurring in soil samples. The procedure for the simultaneous determination of the nine haloacetic acids was simple and rapid. The method detection limits for MFA, DFA, TFA, MCA, DCA, TCA, MBA, DBA, and TBA were 21, 40, 40, 28, 48, 86, 67, 55, and 160 μg L−1, respectively. Application of this method to the determination of haloacetic acids in real soil samples is illustrated.

scholarly journals Determination of Carbohydrates in Soluble (Instant) Coffee by Anion-Exchange Chromatography with Pulsed Amperometric Detection: Summary of Collaborative Study

1996 ◽  
Vol 79 (6) ◽  
pp. 1400-1407
Author(s):  
Jacques Prodolliet ◽  
Emmanuel Bugner ◽  
Max Feevberg
Keyword(s):  
Anion Exchange ◽  
Pure Water ◽  
Collaborative Study ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Free Form ◽  
Individual Values ◽  
Anion Exchange Column ◽  
Instant Coffee

Abstract A collaborative study was conducted to validate a liquid chromatographic (LC) method for determining free and total (after acid hydrolysis) carbohydrate profile of soluble (instant) coffee. Carbohydrates were separated on a pellicular anion-exchange column with pure water as mobile phase and detected by pulsed amperometry. Precisions in determining free and total carbohydrates were very similar. Average RSDr and RSDR values were 4.5 and 14.3%, respectively, for carbohydrate levels >0.3%, with individual values ranging, respectively, from 2.2 to 4.6% and 9.9 to 24.2% for mannitol, 1.6 to 7.3% and 4.9 to 21.1 % for arabinose, 1.7 to 8.1 % and 4.1 to 12.9% for galactose, 2.4 to 8.7% and 6.1 to 24.3% for glucose, 1.8 to 6.8% and 10.0 to 11.6% for sucrose, 3.7 to 7.4% and 22.5 to 27.8% for xylose, 2.0 to 7.0% and 10.6 to 24.4% for mannose, and 2.9 to 5.2% and 15.5 to 18.4% for fructose (free form only). The technique's precision was considered good, taking into account the usual peak integration problems always encountered in LC procedures, the low levels of free carbohydrates, the hydrolysis step, and the relative lack of experience of most participating laboratories. Except for the pair rhamnose/arabinose, the method allows good and reproducible separation of carbohydrates found in soluble coffee and, therefore, is suitable for routine analysis. The anion-exchange chromatographic method with pulsed amperometry for determining carbohydrates in soluble (instant) coffee has been adopted first action by AOAC INTERNATIONAL.

Analysis of Urine for Its Ultraviolet-Absorbing Constituents by High-Pressure Anion-Exchange Chromatography

1968 ◽  
Vol 14 (6) ◽  
pp. 521-528 ◽  
Author(s):  
Charles D Scott
Keyword(s):  
High Pressure ◽  
Anion Exchange ◽  
Digital Voltmeter ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Anion Exchange Column ◽  
Chart Recorder ◽  
Total Analysis ◽  
Chromatographic Peaks ◽  
Normal Urine

Abstract An automatic, high-resolution analytic system for the quantitative determination of the ultraviolet-absorbing molecular constituents of urine and other body fluids is being developed. This system is comprised of a heated, high-pressure anion-exchange column for the separation step and a continuous-flow ultraviolet spectrophotometer for the detection step. Separation is achieved by elution with an acetate buffer of gradually increasing concentration. The total analysis time is 40 hr. Chromatograms showing the absorbance of the column effluent as a function of time are recorded by a strip-chart recorder and by a data acquisition system containing a digital voltmeter and paper tape punch. The output from the latter is then used as the input to a digital computer for analysis of the chromatogram. More than 140 chromatographic peaks have been resolved from a 2-ml. urine sample. Normal urine samples yield similar chromatograms. A variation in the diurnal cycle has been noted.

Correlation between protein desorption behavior and its adsorption enthalpy change in polymer grafted anion exchange chromatography

2021 ◽  
pp. 111853
Author(s):  
Joao Carlos Simoes-Cardoso ◽  
Nanako Hoshino ◽  
Yusuke Yoshimura ◽  
Chyi-Shin Chen ◽  
Cristina Dias-Cabral ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Enthalpy Change ◽  
Adsorption Enthalpy
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