The Effect of Age on Deoxyribonucleic Acid Synthesis in Rat Adipose Tissue

1975 ◽  
Vol 48 (4) ◽  
pp. 323-325
Author(s):  
B. M. Lewis ◽  
T. M. Hayes
Keyword(s):  
Adipose Tissue ◽  
Deoxyribonucleic Acid ◽  
Age Groups ◽  
Acid Synthesis ◽  
Fat Cells ◽  
Deoxyribonucleic Acid Synthesis ◽  
Old Rats ◽  
Rat Adipose Tissue ◽  
Effect Of Age ◽  
Fat Pads

1. Rats of four different age groups were injected intraperitoneally with labelled thymidine and killed 1, 7 or 12 days later. 2. The epididymal fat-pads were separated into fat-cells and stromal elements by collagenase digestion. 3. The incorporation of labelled thymidine into the deoxyribonucleic acid (DNA) of both fractions was greatest in the 6-week-old animals. Uptake was significantly decreased in 12- and 15-week-old animals and was lowest in 22-week-old rats.

scholarly journals Evidence that insulin activates casein kinase 2 in rat epididymal fat-cells and that this may result in the increased phosphorylation of an acid-soluble 22 kDa protein

1991 ◽  
Vol 279 (2) ◽  
pp. 545-551 ◽  
Author(s):  
T A Diggle ◽  
C Schmitz-Peiffer ◽  
A C Borthwick ◽  
G I Welsh ◽  
R M Denton
Keyword(s):  
Adipose Tissue ◽  
Casein Kinase ◽  
Casein Kinase 2 ◽  
Fat Cells ◽  
Isolated Fat Cells ◽  
Peptide Substrate ◽  
Heat Stable ◽  
Epididymal Fat ◽  
Rat Adipose Tissue ◽  
Fat Pads

Casein kinase 2 activity as measured by phosphorylation of the peptide substrate Arg-Arg-Arg-Glu-Glu-Glu-Thr-Glu-Glu-Glu is increased by about 50% in extracts from insulin-treated epididymal fat-pads or isolated fat-cells after purification by Mono Q chromatography. Insulin acts to increase the Vmax. of the kinase. An acid-soluble protein with an apparent subunit molecular mass of about 22 kDa appears to be a substrate for casein kinase 2. The protein possesses a number of properties in common with the acid-soluble heat-stable 22 kDa protein which exhibits increased phosphorylation in rat adipose tissue exposed to insulin.

scholarly journals Effects of Prolactin in Vitro on Fatty Acid Synthesis in Rat Adipose Tissue

1959 ◽  
Vol 234 (12) ◽  
pp. 3111-3114 ◽  
Author(s):  
Albert I. Winegrad ◽  
Walter N. Shaw ◽  
Francis D.W. Lukens ◽  
William C. Stadie
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Rat Adipose Tissue

The influence of radiomimetic substances on deoxyribonucleic acid synthesis and function studied in Escherichia coli / phage systems V. A comparison of the inactivation of phages T 2, T 7 and two strains of T 4 by alkylating agents

1959 ◽  
Vol 151 (942) ◽  
pp. 148-155 ◽  
Keyword(s):  
Deoxyribonucleic Acid ◽  
Osmotic Shock ◽  
Alkylating Agents ◽  
Acid Synthesis ◽  
Acid Moiety ◽  
Rate Of Penetration ◽  
Injection Process ◽  
Deoxyribonucleic Acid Synthesis ◽  
And Function ◽  
Escherichia Coli Phage

The sensitivity of phage T 7 to epoxides and freshly prepared solutions of di(2-chloroethyl) methylamine ( HN 2) was identical with that of T 2. T 7, however, proved considerably the more sensitive to ethylenimine and to aged solutions of HN 2. It was considered that this was due to the cationic nature of these latter agents affecting the rate of penetration into the phage heads, and that the susceptibility of T 2 and resistance of T 7 to osmotic shock was a parallel phenomenon. Confirmation was afforded by the fact that a strain of T 4 sensitive to osmotic shock behaved like T 2, and a resistant strain of T 4 like T 7. These results, together with others previously reported, are believed to offer very strong evidence that inactivation of bacteriophage by alkylating agents derives from reaction with the deoxyribonucleic acid moiety, probably leading to a failure of the injection process.

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