Studies of the Kinetics of Competitive Protein Binding Assays of Cholecalciferol and of 25-Hydroxycholecalciferol

1972 ◽  
Vol 42 (4) ◽  
pp. 16P-17P
Author(s):  
M. A. Preece ◽  
J. L. H. O'Riordan ◽  
D. E. M. Lawson ◽  
S. Edelstein ◽  
E. Kodicek
Keyword(s):  
Protein Binding ◽  
Binding Assays ◽  
Competitive Protein Binding ◽  
Kinetics Of ◽  
25 Hydroxycholecalciferol

A Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1975 ◽  
Vol 17 (2) ◽  
pp. 57-57
Author(s):  
Yoshiki Seino ◽  
Tsunesuke Shimotsuji ◽  
Shintaro Okada ◽  
Teisuke Hiejima ◽  
Chiiko Ikehara ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
25 Hydroxycholecalciferol

Competitive Protein-Binding Assay for 25-Hydroxycholecalciferol

1974 ◽  
Vol 46 (2) ◽  
pp. 231-240 ◽  
Author(s):  
S. Edelstein ◽  
M. Charman ◽  
D. E. M. Lawson ◽  
E. Kodicek
Keyword(s):  
Protein Binding ◽  
Binding Assay ◽  
Rat Serum ◽  
Male And Female ◽  
Normal Volunteers ◽  
Protein Binding Assay ◽  
Competitive Protein Binding Assay ◽  
Competitive Protein Binding ◽  
The Mean ◽  
25 Hydroxycholecalciferol

1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 ± SD 14 pmol/ml (15–2 ± 5–6 ng/ml). 3. Levels of 25-hydroxycholecalciferol in plasma of four male volunteers maintained for 2 months on a diet supplemented with 26·1 nmol (10 μg) of cholecalciferol a day were found to be significantly higher. 4. High correlation was established between plasma 25-hydroxycholecalciferol values obtained by the competitive protein-binding assay and values obtained by bioassay.

Optimal Statistical Design of Radioimmunoassays and Competitive Protein-Binding Assays

Endocrinology ◽  
1975 ◽  
Vol 96 (4) ◽  
pp. 973-981 ◽  
Author(s):  
POTTER C. CHANG ◽  
ROBERT T. RUBI ◽  
MIMI YU
Keyword(s):  
Protein Binding ◽  
Statistical Design ◽  
Binding Assays ◽  
Competitive Protein Binding
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