A Simple Method of Measuring Transmucosal P.D. in Man and its Clinical Application

1970 ◽  
Vol 38 (2) ◽  
pp. 11P-11P ◽  
Author(s):  
C. J. Edmonds ◽  
R. Godfrey
Keyword(s):  
Clinical Application ◽  
Simple Method

Arylesterase in serum: elaboration and clinical application of a fixed-incubation method.

1979 ◽  
Vol 25 (10) ◽  
pp. 1714-1720 ◽  
Author(s):  
K Lorentz ◽  
B Flatter ◽  
E Augustin
Keyword(s):  
Clinical Application ◽  
Gel Electrophoresis ◽  
Reduced Glutathione ◽  
Competitive Inhibitor ◽  
Potassium Ferricyanide ◽  
Phenyl Acetate ◽  
Complete Protection ◽  
Normal Range ◽  
Simple Method ◽  
Incubation Method

Abstract A sensitive, specific, and simple method for determining serum or urine arylesterase (EC 3.1.1.2) is described. The enzyme acts on phenyl acetate to release phenol, which produces a stable indophenol dye with 4-aminoantipyrine and potassium ferricyanide. Arylesterase, a thiol enzyme, is reactivated by 2-mercaptoethanol and by cysteine, but not by reduced glutathione. Calcium is indispensable to stabilize and to activate (Km = 0.85 mmol/L) the enzyme; complete protection is achieved at CaCl2 20 mmol/L. Magnesium acts as a weak (Ki = 116 mmol/L), lanthanum as a potent (Ki = 5 mumol/L) competitive inhibitor. The activity is measured in diluted sera at phenyl acetate 4.0 mmol/L (Km = 1.12 mmol/L), pH 7.8 and 25 degrees C. The normal range extends from 53 to 186 kU/L, and four isoenzymes are present in sera from healthy adults. Arylesterase decreases in hepatic disorders, especially in cirrhosis and carcinoma of the liver, with reduction of the penultimate fraction in polyacryalmide gel electrophoresis.

scholarly journals Titanium mesh for bone augmentation in oral implantology: current application and progress

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Yu Xie ◽  
Songhang Li ◽  
Tianxu Zhang ◽  
Chao Wang ◽  
Xiaoxiao Cai
Keyword(s):  
Clinical Application ◽  
Research Progress ◽  
Bone Augmentation ◽  
Titanium Mesh ◽  
Current Application ◽  
Simple Method ◽  
Barrier Membranes ◽  
Clinical Procedures ◽  
The Difference ◽  
Prevention Methods

AbstractGuided bone regeneration (GBR) is an effective and simple method for bone augmentation, which is often used to reconstruct the alveolar ridge when the bone defect occurs in the implant area. Titanium mesh has expanded the indications of GBR technology due to its excellent mechanical properties and biocompatibility, so that the GBR technology can be used to repair alveolar ridges with larger bone defects, and can obtain excellent and stable bone augmentation results. Currently, GBR with titanium mesh has various clinical applications, including different clinical procedures. Bone graft materials, titanium mesh covering methods, and titanium mesh fixing methods are also optional. Moreover, the research of GBR with titanium mesh has led to multifarious progresses in digitalization and material modification. This article reviews the properties of titanium mesh and the difference of titanium mesh with other barrier membranes; the current clinical application of titanium mesh in bone augmentation; common complications and management and prevention methods in the application of titanium mesh; and research progress of titanium mesh in digitization and material modification. Hoping to provide a reference for further improvement of titanium mesh in clinical application and related research of titanium mesh.

Establishment of Human Adipose Tissue-Derived Stromal Cell Lines: A Culture System to Manufacture Megakaryocytes Releasing Functional Platelets

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 656-656 ◽  
Author(s):  
Keiichi Tozawa ◽  
Yukako Ono-Uruga ◽  
Noriko Takizawa ◽  
Tadashi Horiuchi ◽  
Shin-ichiro Okamoto ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Cell Line ◽  
Cell Lines ◽  
Clinical Application ◽  
Light Transmission ◽  
Conflicts Of Interest ◽  
Clot Retraction ◽  
Fat Cell ◽  
Simple Method

Abstract We have described the use of adipose tissue-derived stromal (ADS) cells (also known as pre-adipocytes) to generate megakaryocytes (MKs) and platelets (Plts) for potential application as non-donor-derived Plts in transfusion medicine. ADS cells are an attractive candidate cell source, because (1) their differentiation does not require gene transfer as they utilize endogenous genes in differentiation into functional MKs, (2) megakaryopoiesis and thrombopoiesis are dependent on endogenous thrombopoietin, and (3) ADS cells are genetically stable in long-term culture. However, Plt yield remains insufficient for clinical application. In the present study, we focused on the establishment and characterization of cell lines from small amount of human ADS cells (5 x 105 cells isolated from ~0.5 g fat). To clarify the usefulness of ADS cell line (ASCL) as a source for clinical application, characterization of ASCL and platelet function tests were performed on ASCL-derived MKs and Plts. We modified the established method, utilizing an upside-down culture flask method (Yagi et al, BBRC 2004), to obtain fat cell line with multipotent capacity. Instead of using large volume of fat as described in the original method, we developed ASCL from a small number of ADS cells, because ADS cells possess the capacity of expansion and differentiation into mature adipocytes rapidly. These ASCL have now been expanded in culture at least 6 months. Gene expression analysis by quantitative real-time PCR indicated that ASCL expressed the genes for several pluripotent cell-markers, such as, OCT3/4, KLF4, Myc, Nanog, Gal, and GABRB3. Also, ASCL expressed genes related to MK lineages, such as p45NF-E2, RUNX1, GATA2, Fli1, FOG1, TPO, and c-MPL. These phenotypes did not differ from ADS cells. These cells were then cultured in MK induction media consisting of IMDM containing transferrin. The frequency of CD41+/CD42b+ MK-sized cells on day 10 was 24 ± 2%. DNA polyploidy, a hallmark of MKs, in CD41+ cells ranged from 2N to 16N with an average of 4.3N. These results were similar to MKs derived directly from ADS cells. The frequency of CD41+/CD42b+ Plt-sized particles on day 18 was 34 ± 6%. For Plt yield, ~30 Plts were obtained from single ASCL. Analysis of granule contents showed that ASCL-derived Plts contain ATP and ADP, and von Willebrand factor. We next examined functionality of these ASCL-derived Plts. Donor Plts were used as a control. Upon stimulation with thrombin (0.5 U/mL), binding of labeled fibrinogen occurred to both ASCL-derived Plts and donor Plts. But binding to ASCL-derived Plts was higher (p<0.001). We also tested PAC1 binding, a marker for Plt activation. Similar to fibrinogen binding, binding to ASCL-derived Plts was higher than to donor Plts (p<0.001). Also, ASCL-derived Plts spread on fibrinogen-coated glass in the presence of ADP/PAR1 (10 μM each), forming lamellipodia stained with anti-beta tubulin antibody and phalloidin. Clot retraction was observed upon stimulation (0.5 U/mL thrombin, 1.5 mM calcium, 100 μg/mL fibrinogen) of both ASCL-derived Plts and donor Plts. Sufficient number of Plts were obtained to perform Plt aggregation study under light transmission aggregometry. Agonist (ADP 20 μM, calcium 1.5 mM)-induced Plt aggregation was observed with ASCL-derived Plts, but not ASCL-derived CD41- particles. Taken together, we established human ADS cell line, ASCL. They retain the ability to proliferate and differentiate into Plts for at least 6 months, and therefore, may be suitable for manufacturing functional Plts by simple method. Disclosures No relevant conflicts of interest to declare.

A Simple Method for Clinical Application of Push/Pull Hemodiafiltration

2015 ◽  
pp. 71-78 ◽  
Author(s):  
Kazuma Tsuruta ◽  
Fumihiro Andoh ◽  
Ikuko Kurahara ◽  
Tetsuya Kaku ◽  
Jun Fukushima ◽  
...  
Keyword(s):  
Clinical Application ◽  
Simple Method

Progesterone in Nonpregnancy Plasma: An Assay Method for the Clinical Chemistry Laboratory

1970 ◽  
Vol 16 (10) ◽  
pp. 856-860 ◽  
Author(s):  
Aron O Lurie ◽  
Richard J Patterson
Keyword(s):  
Menstrual Cycle ◽  
Clinical Application ◽  
Coefficient Of Variation ◽  
Clinical Chemistry ◽  
Chemistry Laboratory ◽  
Assay Method ◽  
Simple Method ◽  
Normal Menstrual Cycle ◽  
Clinical Chemistry Laboratory

Abstract A simple method for the assay of progesterone in nonpregnancy plasma was designed specifically for the clinical chemistry laboratory. One technician can readily perform 22 assays in less than one work day. Although no chromatographic step was used, the method was highly specific. Repro-ducibility studies indicated that the assay had a coefficient of variation of 7.5% for pregnancy plasma, 11.5% for nonpregnancy plasma. Clinical application of the method to specimens obtained from subjects during the normal menstrual cycle yielded values very similar to those obtained by more laborious and costly assay techniques.

Echocardiography. Clinical application in mitral stenosis

JAMA ◽  
1966 ◽  
Vol 195 (3) ◽  
pp. 161-166 ◽  
Author(s):  
B. L. Segal
Keyword(s):  
Clinical Application ◽  
Mitral Stenosis

A simple way for producing holographic filters suitable for image improvement

1978 ◽  
Vol 36 (1) ◽  
pp. 226-227
Author(s):  
K.-H. Herrmann ◽  
E. Reuber ◽  
P. Schiske
Keyword(s):  
Transfer Functions ◽  
Diffraction Order ◽  
Lateral Position ◽  
Simple Method ◽  
Spatial Frequencies ◽  
Resolution Electron ◽  
Wiener Filters ◽  
Image Improvement ◽  
Optical Reconstruction ◽  
Set Up

Aposteriori deblurring of high resolution electron micrographs of weak phase objects can be performed by holographic filters [1,2] which are arranged in the Fourier domain of a light-optical reconstruction set-up. According to the diffraction efficiency and the lateral position of the grating structure, the filters permit adjustment of the amplitudes and phases of the spatial frequencies in the image which is obtained in the first diffraction order.In the case of bright field imaging with axial illumination, the Contrast Transfer Functions (CTF) are oscillating, but real. For different imageforming conditions and several signal-to-noise ratios an extensive set of Wiener-filters should be available. A simple method of producing such filters by only photographic and mechanical means will be described here.A transparent master grating with 6.25 lines/mm and 160 mm diameter was produced by a high precision computer plotter. It is photographed through a rotating mask, plotted by a standard plotter.

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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