Valproic acid eliminates quiescent cancer stem-like cells in pediatric GBMS by driving them into cell cycle and promoting radiation induced-DNA damages: anin vivostudy in orthotopic xenograft models

2010 ◽  
Vol 34 (8) ◽  
pp. S40-S40
Author(s):  
Zhigang Liu ◽  
Yunfei Xia ◽  
Xiao‑Nan Li
Keyword(s):  
Cell Cycle ◽  
Valproic Acid ◽  
Dna Damages ◽  
Orthotopic Xenograft ◽  
Radiation Induced ◽  
Xenograft Models

Intrinsic radiosensitivity correlated with radiation-induced ROS and cell cycle regulation

2010 ◽  
Vol 6 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Ki Moon Seong ◽  
Cha Soon Kim ◽  
Ha Yeon Jeon ◽  
Sin-Hye Oh ◽  
Seon Young Nam ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Intrinsic Radiosensitivity ◽  
Radiation Induced ◽  
Cycle Regulation

scholarly journals A DNA-Damage-Induced Cell Cycle Checkpoint in Arabidopsis

Genetics ◽  
2003 ◽  
Vol 164 (1) ◽  
pp. 323-334
Author(s):  
S B Preuss ◽  
A B Britt
Keyword(s):  
Cell Cycle ◽  
Gamma Radiation ◽  
Cell Cycle Progression ◽  
Cell Cycle Checkpoint ◽  
Phase Cell ◽  
Cycle Arrest ◽  
Cycle Checkpoint ◽  
Radiation Induced ◽  
High Doses ◽  
Regulation Of Cell Cycle

Abstract Although it is well established that plant seeds treated with high doses of gamma radiation arrest development as seedlings, the cause of this arrest is unknown. The uvh1 mutant of Arabidopsis is defective in a homolog of the human repair endonuclease XPF, and uvh1 mutants are sensitive to both the toxic effects of UV and the cytostatic effects of gamma radiation. Here we find that gamma irradiation of uvh1 plants specifically triggers a G2-phase cell cycle arrest. Mutants, termed suppressor of gamma (sog), that suppress this radiation-induced arrest and proceed through the cell cycle unimpeded were recovered in the uvh1 background; the resulting irradiated plants are genetically unstable. The sog mutations fall into two complementation groups. They are second-site suppressors of the uvh1 mutant's sensitivity to gamma radiation but do not affect the susceptibility of the plant to UV radiation. In addition to rendering the plants resistant to the growth inhibitory effects of gamma radiation, the sog1 mutation affects the proper development of the pollen tetrad, suggesting that SOG1 might also play a role in the regulation of cell cycle progression during meiosis.

The essential role of p21 in radiation-induced cell cycle arrest of vascular smooth muscle cell

2004 ◽  
Vol 37 (4) ◽  
pp. 871-880 ◽  
Author(s):  
Hyo-Soo Kim ◽  
Hyun-Jai Cho ◽  
Hyun-Ju Cho ◽  
Sun-Jung Park ◽  
Kyung-Woo Park ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Cell Cycle Arrest ◽  
Muscle Cell ◽  
Essential Role ◽  
Cycle Arrest ◽  
Radiation Induced

scholarly journals Live-cell imaging to detect phosphatidylserine externalization in brain endothelial cells exposed to ionizing radiation: implications for the treatment of brain arteriovenous malformations

2016 ◽  
Vol 124 (6) ◽  
pp. 1780-1787 ◽  
Author(s):  
Zhenjun Zhao ◽  
Michael S. Johnson ◽  
Biyi Chen ◽  
Michael Grace ◽  
Jaysree Ukath ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Endothelial Cells ◽  
Ionizing Radiation ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Vascular Targeting ◽  
Radiation Doses ◽  
Radiation Induced ◽  
Polarity Sensitive

OBJECT Stereotactic radiosurgery (SRS) is an established intervention for brain arteriovenous malformations (AVMs). The processes of AVM vessel occlusion after SRS are poorly understood. To improve SRS efficacy, it is important to understand the cellular response of blood vessels to radiation. The molecular changes on the surface of AVM endothelial cells after irradiation may also be used for vascular targeting. This study investigates radiation-induced externalization of phosphatidylserine (PS) on endothelial cells using live-cell imaging. METHODS An immortalized cell line generated from mouse brain endothelium, bEnd.3 cells, was cultured and irradiated at different radiation doses using a linear accelerator. PS externalization in the cells was subsequently visualized using polarity-sensitive indicator of viability and apoptosis (pSIVA)-IANBD, a polarity-sensitive probe. Live-cell imaging was used to monitor PS externalization in real time. The effects of radiation on the cell cycle of bEnd.3 cells were also examined by flow cytometry. RESULTS Ionizing radiation effects are dose dependent. Reduction in the cell proliferation rate was observed after exposure to 5 Gy radiation, whereas higher radiation doses (15 Gy and 25 Gy) totally inhibited proliferation. In comparison with cells treated with sham radiation, the irradiated cells showed distinct pseudopodial elongation with little or no spreading of the cell body. The percentages of pSIVA-positive cells were significantly higher (p = 0.04) 24 hours after treatment in the cultures that received 25- and 15-Gy doses of radiation. This effect was sustained until the end of the experiment (3 days). Radiation at 5 Gy did not induce significant PS externalization compared with the sham-radiation controls at any time points (p > 0.15). Flow cytometric analysis data indicate that irradiation induced growth arrest of bEnd.3 cells, with cells accumulating in the G2 phase of the cell cycle. CONCLUSIONS Ionizing radiation causes remarkable cellular changes in endothelial cells. Significant PS externalization is induced by radiation at doses of 15 Gy or higher, concomitant with a block in the cell cycle. Radiation-induced markers/targets may have high discriminating power to be harnessed in vascular targeting for AVM treatment.

scholarly journals Cell Cycle and LET Dependence for Radiation-induced Mutation. A Possible Mechanism for Reversed Dose-rate Effect.

1999 ◽  
Vol 40 (Suppl.) ◽  
pp. 45-52 ◽  
Author(s):  
HIROSHI TAUCHI ◽  
SATORU ENDO ◽  
KIYOMI EGUCHI-KASAI ◽  
YOSHIYA FURUSAWA ◽  
MASAO SUZUKI ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dose Rate ◽  
Rate Effect ◽  
Induced Mutation ◽  
Radiation Induced ◽  
Dose Rate Effect

Patient-derived orthotopic xenograft models of sarcoma

2020 ◽  
Vol 469 ◽  
pp. 332-339 ◽  
Author(s):  
Kentaro Igarashi ◽  
Kei Kawaguchi ◽  
Takashi Murakami ◽  
Kentaro Miyake ◽  
Tasuku Kiyuna ◽  
...  
Keyword(s):  
Orthotopic Xenograft ◽  
Xenograft Models

scholarly journals Exploring the Drug Repurposing Versatility of Valproic Acid as a Multifunctional Regulator of Innate and Adaptive Immune Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-24 ◽  
Author(s):  
Rodolfo Soria-Castro ◽  
Alejandro Schcolnik-Cabrera ◽  
Gloria Rodríguez-López ◽  
Marcia Campillo-Navarro ◽  
Nahum Puebla-Osorio ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Valproic Acid ◽  
Immune Cells ◽  
Histone Deacetylases ◽  
Drug Repurposing ◽  
Promising Alternative ◽  
Expression Of Genes ◽  
Adaptive Immune Cells ◽  
Activation Of Transcription ◽  
Activation Mechanisms

Valproic acid (VPA) is widely recognized for its use in the control of epilepsy and other neurological disorders in the past 50 years. Recent evidence has shown the potential of VPA in the control of certain cancers, owed in part to its role in modulating epigenetic changes through the inhibition of histone deacetylases, affecting the expression of genes involved in the cell cycle, differentiation, and apoptosis. The direct impact of VPA in cells of the immune system has only been explored recently. In this review, we discuss the effects of VPA in the suppression of some activation mechanisms in several immune cells that lead to an anti-inflammatory response. As expected, immune cells are not exempt from the effect of VPA, as it also affects the expression of genes of the cell cycle and apoptosis through epigenetic modifications. In addition to inhibiting histone deacetylases, VPA promotes RNA interference, activates histone methyltransferases, or represses the activation of transcription factors. However, during the infectious process, the effectiveness of VPA is subject to the biological nature of the pathogen and the associated immune response; this is because VPA can promote the control or the progression of the infection. Due to its various effects, VPA is a promising alternative for the control of autoimmune diseases and hypersensitivity and needs to be further explored.

Sign in / Sign up

Export Citation Format

Share Document