scholarly journals Copper transport and regulation in Schizosaccharomyces pombe

2013 ◽  
Vol 41 (6) ◽  
pp. 1679-1686 ◽  
Author(s):  
Jude Beaudoin ◽  
Seda Ekici ◽  
Fevzi Daldal ◽  
Samia Ait-Mohand ◽  
Brigitte Guérin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Plasma Membrane ◽  
Schizosaccharomyces Pombe ◽  
Copper Metabolism ◽  
Eukaryotic Cells ◽  
Stepping Stones ◽  
Copper Transporter ◽  
Fundamental Knowledge ◽  
Membrane Complex ◽  
Related Proteins

The fission yeast Schizosaccharomyces pombe has been successfully used as a model to gain fundamental knowledge in understanding how eukaryotic cells acquire copper during vegetative growth. These studies have revealed the existence of a heteromeric Ctr4–Ctr5 plasma membrane complex that mediates uptake of copper within the cells. Furthermore, additional studies have led to the identification of one of the first vacuolar copper transporters, Ctr6, as well as the copper-responsive Cuf1 transcription factor. Recent investigations have extended the use of S. pombe to elucidate new roles for copper metabolism in meiotic differentiation. For example, these studies have led to the discovery of Mfc1, which turned out to be the first example of a meiosis-specific copper transporter. Whereas copper-dependent transcriptional regulation of the Ctr family members is under the control of Cuf1 during mitosis or meiosis, meiosis-specific copper transporter Mfc1 is regulated by the recently discovered transactivator Mca1. It is foreseeable that identification of novel meiotic copper-related proteins will serve as stepping stones to unravel fundamental aspects of copper homoeostasis.

scholarly journals Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis

2017 ◽  
Vol 216 (10) ◽  
pp. 3041-3050 ◽  
Author(s):  
Chloe E. Snider ◽  
Alaina H. Willet ◽  
Jun-Song Chen ◽  
Göker Arpağ ◽  
Marija Zanic ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cell Division ◽  
Schizosaccharomyces Pombe ◽  
Lipid Composition ◽  
Eukaryotic Cells ◽  
Central Position ◽  
Dependent Manner ◽  
Myosin V ◽  
Contractile Ring ◽  
Phosphatidylinositol 4

Many eukaryotic cells divide by assembling and constricting an actin- and myosin-based contractile ring (CR) that is physically linked to the plasma membrane (PM). In this study, we report that Schizosaccharomyces pombe cells lacking efr3, which encodes a conserved PM scaffold for the phosphatidylinositol-4 kinase Stt4, build CRs that can slide away from the cell middle during anaphase in a myosin V–dependent manner. The Efr3-dependent CR-anchoring mechanism is distinct from previously reported pathways dependent on the Fes/CIP4 homology Bin-Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Pxl1. In efr3Δ, the concentrations of several membrane-binding proteins were reduced in the CR and/or on the PM. Our results suggest that proper PM lipid composition is important to stabilize the central position of the CR and resist myosin V–based forces to promote the fidelity of cell division.

scholarly journals Molecular architecture of the endocytic TPLATE complex

2021 ◽  
Vol 7 (9) ◽  
pp. eabe7999
Author(s):  
Klaas Yperman ◽  
Jie Wang ◽  
Dominique Eeckhout ◽  
Joanna Winkler ◽  
Lam Dai Vu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Structural Approach ◽  
Eukaryotic Cells ◽  
Molecular Architecture ◽  
Membrane Proteome ◽  
Complex Assembly ◽  
Membrane Interaction ◽  
Structural Insight ◽  
Fresh Insight ◽  
Insight Into

Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generate fresh insight into the differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.

scholarly journals Transcription Factor GATA-6, Cell Proliferation, Apoptosis, and Apoptosis-Related Proteins Bcl-2 and Bax in Human Fetal Testis

2003 ◽  
Vol 88 (4) ◽  
pp. 1858-1865 ◽  
Author(s):  
Ilkka Ketola ◽  
Jorma Toppari ◽  
Tommi Vaskivuo ◽  
Riitta Herva ◽  
Juha S. Tapanainen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcription Factor ◽  
Fetal Testis ◽  
Related Proteins

A mass spectrometric approach to identify arbuscular mycorrhiza-related proteins in root plasma membrane fractions

PROTEOMICS ◽  
2006 ◽  
Vol 6 (S1) ◽  
pp. S145-S155 ◽  
Author(s):  
Benoît Valot ◽  
Luc Negroni ◽  
Michel Zivy ◽  
Silvio Gianinazzi ◽  
Eliane Dumas-Gaudot
Keyword(s):  
Plasma Membrane ◽  
Arbuscular Mycorrhiza ◽  
Mass Spectrometric ◽  
Mass Spectrometric Approach ◽  
Related Proteins ◽  
Root Plasma Membrane

Autoregulated Expression of Schizosaccharomyces pombe Meiosis-Specific Transcription Factor Mei4 and a Genome-Wide Search for Its Target Genes

Genetics ◽  
2000 ◽  
Vol 154 (4) ◽  
pp. 1497-1508 ◽  
Author(s):  
Hiroko Abe ◽  
Chikashi Shimoda
Keyword(s):  
Transcription Factor ◽  
Schizosaccharomyces Pombe ◽  
Target Genes ◽  
Northern Blotting ◽  
Forkhead Transcription Factor ◽  
Gene Encoding ◽  
Putative Promoter Region ◽  
Cis Acting ◽  
A Genome ◽  
Genome Wide Search

Abstract The Schizosaccharomyces pombe mei4+ gene encoding a forkhead transcription factor is necessary for the progression of meiosis and sporulation. We searched for novel meiotic genes, the expression of which is dependent on Mei4p, since only the spo6+ gene has been assigned to its targets. Six known genes responsible for meiotic recombination were examined by Northern blotting, but none were Mei4 dependent for transcription. We determined the important cis-acting element, designated FLEX, to which Mei4p can bind. The S. pombe genome sequence database (The Sanger Centre, UK) was scanned for the central core heptamer and its flanking 3′ sequence of FLEX composed of 17 nucleotides, and 10 candidate targets of Mei4 were selected. These contained a FLEX-like sequence in the 5′ upstream nontranslatable region within 1 kb of the initiation codon. Northern blotting confirmed that 9 of them, named mde1+ to mde9+, were transcriptionally induced during meiosis and were dependent on mei4+. Most mde genes have not been genetically defined yet, except for mde9+, which is identical to spn5+, which encodes one of the septin family of proteins. mde3+ and a related gene pit1+ encode proteins related to Saccharomyces cerevisiae Ime2. The double disruptant frequently produced asci having an abnormal number and size of spores, although it completed meiosis. We also found that the forkhead DNA-binding domain of Mei4p binds to the FLEX-like element in the putative promoter region of mei4 and that the maximum induction level of mei4 mRNA required functional mei4 activity. Furthermore, expression of a reporter gene driven by the authentic mei4 promoter was induced in vegetative cells by ectopic overproduction of Mei4p. These results suggest that mei4 transcription is positively autoregulated.

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